Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Ad!dict --- KAN design consultants --- LC design --- Vandekerkhove & Devos

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Fysicochemical separation methods --- kolomchromatografie --- affiniteitschromatografie --- LC (liquid chromatography) --- chromatografie

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

This volume provides a description of LC/MS applications that are currently being successfully used and of the instrumentation that is now commercially available or under development. Describes the application of LC/MS to the analysis of agricultural chemicals and their metabolites. Examines the influence LC/MS has had upon chemistry for the analysis of both highly polar metabolites and their precursors. Also looks at the impact of LC/MS methods on environmental analysis.

spectrometrie --- LC (liquidchromatography) --- pharmacology --- massaspectrometrie --- farmacologie --- landbouw --- Pharmacology. Therapy --- milieu --- Fysicochemical separation methods --- Liquid chromatography --- Mass spectrometry --- Chemistry --- Physical Sciences & Mathematics --- Analytical Chemistry --- Congresses --- Congresses. --- LC (liquid chromatography)

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Thin-layer chromatography (TLC) is widely used particularly for pharmaceutical and food analysis. While there are a number of books on the qualitative identification of chemical substances by TLC, the unique focus here is on quantitative analysis. The authors describe all steps of the analytical procedure, beginning with the basics and equipment for quantitative TLC followed by sample pretreatment and sample application, development and staining, scanning, and finally statistical and chemometric data evaluation and validation. An important feature is the coverage of effect-directed biological detection methods. Chapters are organized in a modular fashion facilitating the easy location of information about individual procedural steps.

Pharmacology. Therapy --- Food science and technology --- farmacologie --- voedingsleer --- LC (liquid chromatography) --- chromatografie --- DLC (dunnelaagchromatografie)

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

This book focuses on an “outside the box” notion by utilizing the powerful applications of next-generation sequencing (NGS) technologies in the interface of chemistry and biology. In personalized medicine, developing small molecules targeting a specific genomic sequence is an attractive goal. N-methylpyrrole (P)–N-methylimidazole (I) polyamides (PIPs) are a class of small molecule that can bind to the DNA minor groove. First, a cost-effective NGS (ion torrent platform)-based Bind-n-Seq was developed to identify the binding specificity of PIP conjugates in a randomized DNA library. Their biological influences rely primarily on selective DNA binding affinity, so it is important to analyze their genome-wide binding preferences. However, it is demanding to enrich specifically the small-molecule-bound DNA without chemical cross-linking or covalent binding in chromatinized genomes. Herein is described a method that was developed using high-throughput sequencing to map the differential binding sites and relative enriched regions of non-cross-linked SAHA-PIPs throughout the complex human genome. SAHA-PIPs binding motifs were identified and the genome-level mapping of SAHA-PIPs-enriched regions provided evidence for the differential activation of the gene network. A method using high-throughput sequencing to map the binding sites and relative enriched regions of alkylating PIP throughout the human genome was also developed. The genome-level mapping of alkylating the PIP-enriched region and the binding sites on the human genome identifies significant genomic targets of breast cancer. It is anticipated that this pioneering low-cost, high through-put investigation at the sequence-specific level will be helpful in understanding the binding specificity of various DNA-binding small molecules, which in turn will be beneficial for the development of small-molecule-based drugs targeting a genome-level sequence. .

Organic chemistry --- Molecular biology --- Biotechnology --- affiniteitschromatografie --- organische chemie --- biotechnologie --- gentherapie --- LC (liquid chromatography) --- moleculaire biologie