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Electrophoresis, Capillary --- Inositol Phosphates --- methods --- analysis

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Inositol, in its native or lipid derived forms, serves as a master building block which, when phosphorylated, leads to the construction of more than 30 unique isomeric forms that are employed in vital but diverse regulatory roles in cells. In Inositol Phosphates and Lipids: Methods and Protocols, expert researchers introduce the basic methodological tools to measure inositol lipids and phosphates and also describe new approaches that have become available in the last 10 years, including RNA-silencing and the use of fluorescently labeled PH-domains to measure inositides in real-time in live cells, new sensitive methods to measure mass of both phosphates and lipids, as well as protocols involving inositol pyrophosphates. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, detailing tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Inositol Phosphates and Lipids: Methods and Protocols compiles many of the techniques that underscore phosphorylated inositol cell biology in one convenient guide.

Cytology. --- Cell physiology. --- Cell Biology. --- Cell Physiology. --- Cell function --- Cytology --- Physiology --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Inositol phosphates --- Phospholipids --- Inositol Phosphates --- Phosphatidylinositols --- Analysis --- agonists. --- metabolism.

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Ion Channels --- Muscle, Smooth --- Receptors, Purinergic --- Inositol Phosphates --- Calcium --- drug effects --- metabolism

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Human histology. Human cytology --- Histology. Cytology --- Human biochemistry --- Cell regulation --- Cellular control mechanisms --- Celregulatie --- Régulation cellulaire --- Phosphoinositides --- Cellular signal transduction --- Inositol phosphates --- Cellular signal transduction. --- Inositol phosphates. --- Phosphoinositides. --- Inositol --- Metabolism --- Lipids

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This volume describes the current status of the biology of inositols and phosphoinositides with an emphasis on the development in the area since the publication of volume 26 in 1996 in this series. The progress made in dissecting the genetics, structure and evolution of the seminal enzyme for synthesis of inositol in the biological system has driven the understanding of the enzyme forward. With the current genomic and proteomic tools in place the new role of inositols, inositol phosphates and phosphoinositides in cell signaling or stress response has been explored. These advances are described

Inositol. --- Inositol phosphates. --- Phosphoinositides. --- Phosphatidylinositides --- Phosphatidylinositol phosphates --- Phospholipids --- Inositol --- Hexose phosphates --- Cyclohexanehexol --- Mesoinositol --- Myoinositol --- Alcohols --- Phosphoinositides --- Sugars --- Vitamin B complex --- Biochemistry. --- Plant physiology. --- Biochemistry, general. --- Plant Biochemistry. --- Plant Physiology. --- Botany --- Plants --- Physiology --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition --- Plant biochemistry. --- Phytochemistry --- Plant biochemistry --- Plant chemistry --- Biochemistry --- Phytochemicals --- Plant biochemical genetics