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On 20 October 2022, the day that Radboud University celebrated its 99th anniversary, Dr. Katalin Karikó received a Radboud honorary doctorate in recognition of her scientific contributions to developing mRNA-based vaccines. This edition includes the laudatio of the honorary supervisor and the speech of the honorary doctor.Karikó spent years researching medical applications of mRNA. Her dream was to develop synthetic mRNA and use this to cure cancer, strokes, and influenza. Eventually, after years of toil, rejection, and criticism from colleagues, she and fellow researcher Drew Weissman demonstrated that it is possible to trigger an immune response in the body with mRNA without the body turning against the mRNA itself. With this breakthrough, a new revolutionary technique was born.Thanks to Karikó's scientific work, BioNTech/Pfizer and Moderna were able to develop the current mRNA vaccines against COVID-19. A prime example of the beneficial impact that fundamental research can eventually have on society. Honorary supervisor Floris Rutjes, Professor in Organic Synthesis: "With courage and determination, she pursued her scientific vision for a very long time, and by doing so, she has ultimately made a significant contribution to the fight against viral diseases."

Messenger RNA. --- mRNA vaccines. --- Messenger RNA vaccines --- Naked RNA vaccines --- RNA vaccines --- Synthetic vaccines --- Informational RNA --- Messenger ribonucleic acid --- mRNA --- Protein transcript --- Protein transcripts --- Template RNA --- RNA --- Karikó, Katalin. --- Karikó, Kati

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Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors, and mRNA instability elements responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation.

Messenger RNA. --- Genetic regulation. --- Eukaryotic cells. --- Nucleases. --- ARN messager --- Régulation génétique --- Cellules eucaryotes --- Régulation génétique --- Eucaryotic cells --- Gene expression --- Gene expression regulation --- Gene regulation --- Informational RNA --- Messenger ribonucleic acid --- mRNA --- Protein transcript --- Protein transcripts --- Template RNA --- Regulation --- Esterases --- Cells --- Protista --- Biosynthesis --- Cellular control mechanisms --- Molecular genetics --- RNA

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Developments over the past few years have revealed the remarkable versatility of RNA in any compartment of the cell, tasks that had been thought to be exclusively in the realm of proteins and even beyond. The chapters in this book written by leading investigators in the field provide insight into various promising avenues where RNA and nucleic acid derivatives including antisense RNAs, such as siRNA, miRNAs, amplification/selection (SELEX) generated aptamers as well as ribozymes are at the threshold of impacting medicine.

RNA. --- Messenger RNA. --- Informational RNA --- Messenger ribonucleic acid --- mRNA --- Protein transcript --- Protein transcripts --- Template RNA --- RNA --- Ribonucleic acid --- Ribose nucleic acid --- Nucleic acids --- Ribose --- Biochemistry. --- Toxicology. --- Medicine. --- Biochemistry, general. --- Pharmacology/Toxicology. --- Molecular Medicine. --- Medicine/Public Health, general. --- Clinical sciences --- Medical profession --- Human biology --- Life sciences --- Medical sciences --- Pathology --- Physicians --- Chemicals --- Medicine --- Pharmacology --- Poisoning --- Poisons --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Toxicology --- Composition --- Health Workforce --- Pharmacology. --- Molecular biology. --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biophysics --- Biomolecules --- Systems biology --- Drug effects --- Medical pharmacology --- Chemotherapy --- Drugs --- Pharmacy --- Physiological effect --- Medical sciences. --- Biomedical Research. --- Health Sciences. --- Research. --- Basic medical sciences --- Basic sciences, Medical --- Biomedical sciences --- Health sciences --- Preclinical sciences --- Sciences, Medical --- Biological research --- Biomedical research

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mRNA processing is a key step in gene expression that effects all the proteins within the cell. In mRNA Processing and Metabolism: Methods and Protocols, world-renowned researchers bring together the latest techniques spanning the breadth of mRNA processing and metabolism. Drawing on recent advances in microscopy, whole genome sequencing, microarrays, mass spectrometry, fluorescent detection methodologies, and RNA interference, the authors offer readily reproducible methods for the cotranscriptional processing events that occur while the mRNA is engaged with elongating RNA polymerase II, with splicing and its biochemical analysis and with alternative splicing. Additional methods cover mRNA export, the recovery and analysis of mRNP complexes, cytoplasmic translation, mRNA degradation in vivo and in vitro, and the controversial concept of nuclear translation. A variety of model organisms are used, including yeast, Drosophila, Xenopus, mice, plants, and cultured mammalian cells. Each proven protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Timely and authoritative, mRNA Processing and Metabolism: Methods and Protocols provides a powerful collection of novel techniques that are not only effective, but also immediately applicable to current problems in many areas of biological research.

Messenger RNA --- ARN messager --- Laboratory manuals --- Manuels de laboratoire --- Electronic books. -- local. --- Genetics -- Technique. --- Messenger RNA -- Laboratory manuals. --- RNA, Messenger --- Genetic Techniques --- Metabolism --- Metabolic Phenomena --- RNA --- Investigative Techniques --- Nucleic Acids --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Phenomena and Processes --- Nucleic Acids, Nucleotides, and Nucleosides --- Chemicals and Drugs --- Animal Biochemistry --- Human Anatomy & Physiology --- Health & Biological Sciences --- 577.216 --- 57.088.6 --- Transfer of inheritance information. Transport of messenger RNA in the cell --- Methods and techniques for studying metabolism and biotransformation.Radioisotopes methods and techniques. --- 57.088.6 Methods and techniques for studying metabolism and biotransformation.Radioisotopes methods and techniques. --- 577.216 Transfer of inheritance information. Transport of messenger RNA in the cell --- Genetics --- Technique. --- Informational RNA --- Messenger ribonucleic acid --- mRNA --- Protein transcript --- Protein transcripts --- Template RNA --- Methods and techniques for studying metabolism and biotransformation.Radioisotopes methods and techniques --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists