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Immunophenotyping --- Immunophenotyping. --- Flow Cytometry --- Flow cytometry --- methods. --- standards. --- Diagnostic use.
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Le sujet a traité du manque de rentabilité des analyses d'immunophénotypage réalisées en cytométrie en flux au laboratoire d'hématologie du CHU Saint Pierre. L'objectif de ce mémoire est d'une part, de réaliser la description de la situation actuelle de la cytométrie via une analyse partielle des processus et des coûts engendrés par cette activité. Et d'autre part, de proposer des pistes d'amélioration de la gestion technique de la cytométrie. Pour atteindre cet objectif, une analyse de coûts partielle a été menée aux laboratoires d'hématologie du CHU Saint Pierre et l'hôpital Erasme. Dans un deuxième temps, ce mémoire a comparé les deux processus d'analyses d'immunophénotypage suivies dans les deux laboratoires hospitaliers visités. Ces démarches ont eu comme objectif, l'ambition d'améliorer l'efficience de l'activité de la cytométrie en flux du CHU Saint-Pierre. Méthodes : Les coûts des réactifs des 7 types d'analyses d'immunophénotypage, usuellement effectuées au laboratoire pour des patients ambulatoires en diagnostic, ont été décortiqués en coûts par test, par préparation de cocktail et par valorisation. Ces données furent comparées avec celle obtenues au laboratoire d'hématologie de l'hôpital Erasme. Les deux processus étudiés ont été décrits et sectionnés en 8 étapes. Les différences de pratiques y ont été situées. Résultats : Au CHU Saint Pierre, les résultats ont démontrés une variation de coûts entre les analyses effectuées en cytomètre SC et en cytomètre !OC. Les coûts par test d'imrnunophénotypage sont inférieurs à l'hôpital Erasme par rapport au CHU Saint Pierre. De plus, il existe des différences de pratiques de cytométrie en flux entre les deux laboratoires. Ce mémoire a permis l'élaboration de 5 pistes d'amélioration applicables au secteur de cytométrie du CHU Saint Pierre.
Flow Cytometry --- Hematology --- Neoplasms --- Immunophenotyping
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This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact
Medicine --- Immunology --- flow cytometry --- primary immunodeficency --- immunophenotyping --- diagnosis --- functional studies
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This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact
flow cytometry --- primary immunodeficency --- immunophenotyping --- diagnosis --- functional studies
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This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact
Medicine --- Immunology --- flow cytometry --- primary immunodeficency --- immunophenotyping --- diagnosis --- functional studies --- flow cytometry --- primary immunodeficency --- immunophenotyping --- diagnosis --- functional studies
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Cell Differentiation --- Immunophenotyping --- Liver --- Lung --- Multipotent Stem Cells --- Mesoderm --- Stem Cells --- Bone Marrow Transplantation --- Fetal Blood --- physiology --- methods --- embryology --- embryology --- cytology --- cytology --- cytology --- cytology
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Although instrumentation and laboratory techniques for flow cytometry (FCM) immunophenotyping of hematopoietic malignancies are well documented, there is relatively little information on how best to perform data analysis, a critical step in FCM testing. In Flow Cytometry in Hematopathology: A Visual Approach to Data Analysis and Interpretation, three physicians highly experienced in laboratory hematopathology and FCM offer a unique systematic approach to FCM data analysis and interpretation based on the visual inspection of dual parameter FCM graphics. This step-by-step approach to optimal FCM data analysis is demonstrated by means of numerous FCM graphics derived from actual well-documented clinical cases. In this second edition of this well received book, the authors have revised and expanded the text and added more than 100 figures to reflect the recent advances in the field. The focus of the additional material is on the TCR-Vb eight-tube kit which has greatly facilitated the evaluation mature T-cell disorders, and on the DNA dye DRAQ5 for improved grading of malignant lymphoma. The authors also include notes on "tricks of the trade" and pitfalls to avoid. The discussion, covering leukemias, lymphomas, and other conditions, moves from simple to complex specimens, with an emphasis on visual pattern analysis. Richly illustrated and highly instructive, Flow Cytometry in Hematopathology: A Visual Approach to Data Analysis and Interpretation offers clinical pathologists, hematopathologists, and specialists in laboratory medicine a much-needed guide with a practical and logical approach for sharpening their FCM data analysis skills on a wide spectrum of hematologic disorders.
Blood --- Flow cytometry --- Hematopoietic stem cell disorders --- Immunophenotyping --- Pathophysiology. --- Methodology. --- Diagnosis. --- Lymphocyte immunophenotyping --- Lymphocyte subtyping --- Lymphocytes --- Hematopoietic stem cell diseases --- Hematopoietic stem cells --- Cell counting, Laser --- Cell separation, Laser --- Cell sorting, Laser --- Flow cytofluorometry --- Flow cytophotometry --- Laser cell counting --- Laser cell separation --- Laser cell sorting --- Cytometry --- Body fluids --- Fear of blood --- Classification --- Diseases
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The “Atlas Effectors of Anti-Tumor Immunity” is a unique scientific publication, which includes major issues of up-to-date information about immunophenotype, morphology and function of main effectors of anti-tumor immunity presented in a reasonable format. The Atlas comprises a large number of illustrations presented by schemes and original micrographs demonstrating morphological features and ultrastructure of immunocompetent cells at various stages of differentiation. The volume includes data referring to the history of anti-tumor immunity effectors research, state of the art and perspectives for development of anti-cancer adoptive immunotherapy methods. A special section of the Atlas describes cellular tumor microenvironment and micro-anatomy of carcinomas. Several parts include data about killer cells (natural killer T-cells, lymphokine-activated killers) and T-regulatory lymphocytes. A special chapter gives a wide-range description of antigen-presenting dendritic cells, methods of dendritic cell generation and development of DC-based vaccines for anti-tumor immunotherapy. Co-authors of this book are expert scientists of major research centers of the USA and Europe, Atlas contents involves literature data analysis and results of long-term experimental and clinical studies. Atlas may be recommended as an additional study guide for medical and biology students and it may be interesting to experimental and clinical oncologists, immunologists, specialists of research and diagnostic centers.
Tumors --- Tumor antigens. --- Immunophenotyping. --- Immunological aspects. --- Lymphocyte immunophenotyping --- Lymphocyte subtyping --- Lymphocytes --- Antigens --- Tumor markers --- Tumor immunology --- Classification --- Oncology. --- Oncology . --- Immunology. --- Medicine. --- Cancer Research. --- Biomedicine general. --- Clinical sciences --- Medical profession --- Human biology --- Life sciences --- Medical sciences --- Pathology --- Physicians --- Immunobiology --- Serology --- Health Workforce --- Cancer research. --- Biomedicine, general. --- Cancer research
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Since the end of World War II, biology and medicine have merged in remarkably productive ways. In this book Peter Keating and Alberto Cambrosio analyze the transformation of medicine into biomedicine and its consequences, ranging from the recasting of hospital architecture to the redefinition of the human body, disease, and therapeutic practices. To describe this new alignment between the normal and the pathological, the authors introduce the notion of the biomedical platform. Defined as a specific configuration of instruments, individuals, and programs, biomedical platforms generate routines, entities, and activities, held together by standard reagents and protocols. Biological entities such as cell surface markers, oncogenes, and DNA profiles now exist as both normal biological components of the organism and as pathological signs--that is, as biomedical substances. The notion of a biomedical platform allows researchers interested in the development of contemporary medicine to describe events and processes overlooked by other approaches.The authors focus on a specific biomedical platform known as immunophenotyping. They describe its emergence as an experimental system with roots in biology (immunology) and pathology (oncology). They recount how this experimental system was transformed into a biomedical platform initially for the diagnosis of leukemia and subsequently for other diseases such as AIDS. Through this case study, they show that a biomedical platform is the bench upon which conventions concerning the biological or normal are connected with conventions concerning the medical or pathological. They observe that new platforms are often aligned with existing ones and integrated into an expanding set of clinical-biological strategies.
Technology --- Immunologic Tests --- Biological Science Disciplines --- Sociology --- Health Occupations --- Disciplines and Occupations --- Technology, Industry, and Agriculture --- Natural Science Disciplines --- Immunologic Techniques --- Laboratory Techniques and Procedures --- Social Sciences --- Diagnosis --- Technology, Industry, Agriculture --- Behavioral Sciences --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Behavioral Disciplines and Activities --- Psychiatry and Psychology --- Immunophenotyping --- Sociology, Medical --- Biotechnology --- Biotechnologies --- Sociology of Medicine --- Medical Sociology --- Immunologic Subtyping --- Immunologic Subtypings --- Lymphocyte Phenotyping --- Subtyping, Immunologic --- Subtypings, Immunologic --- Lymphocyte Immunophenotyping --- Lymphocyte Subtyping --- Immunophenotyping, Lymphocyte --- Immunophenotypings --- Immunophenotypings, Lymphocyte --- Lymphocyte Immunophenotypings --- Lymphocyte Phenotypings --- Lymphocyte Subtypings --- Phenotyping, Lymphocyte --- Phenotypings, Lymphocyte --- Subtyping, Lymphocyte --- Subtypings, Lymphocyte --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Proxemics --- Behavioral Science --- Proxemic --- Science, Behavioral --- Sciences, Behavioral --- Antemortem Diagnosis --- Diagnoses and Examinations --- Examinations and Diagnoses --- Postmortem Diagnosis --- Antemortem Diagnoses --- Diagnoses --- Diagnoses, Antemortem --- Diagnoses, Postmortem --- Diagnosis, Antemortem --- Diagnosis, Postmortem --- Postmortem Diagnoses --- Disease --- Science, Social --- Sciences, Social --- Social Science --- Antibody Dissociation --- Immunologic Technic --- Immunologic Technics --- Immunologic Technique --- Immunological Technics --- Immunological Techniques --- Technic, Immunologic --- Technics, Immunologic --- Technique, Immunologic --- Techniques, Immunologic --- Antibody Dissociations --- Dissociation, Antibody --- Dissociations, Antibody --- Immunological Technic --- Immunological Technique --- Technic, Immunological --- Technics, Immunological --- Technique, Immunological --- Techniques, Immunological --- Allergy and Immunology --- Natural Sciences --- Physical Sciences --- Discipline, Natural Science --- Disciplines, Natural Science --- Natural Science --- Natural Science Discipline --- Physical Science --- Science, Natural --- Science, Physical --- Sciences, Natural --- Sciences, Physical --- Health Professions --- Health Occupation --- Health Profession --- Occupation, Health --- Occupations, Health --- Profession, Health --- Professions, Health --- General Social Development and Population --- Biologic Sciences --- Biological Science --- Science, Biological --- Sciences, Biological --- Biological Sciences --- Life Sciences --- Biologic Science --- Biological Science Discipline --- Discipline, Biological Science --- Disciplines, Biological Science --- Life Science --- Science Discipline, Biological --- Science Disciplines, Biological --- Science, Biologic --- Science, Life --- Sciences, Biologic --- Sciences, Life --- Diagnosis, Immunologic --- Immunologic Diagnosis --- Immunologic Test --- Immunological Tests --- Tests, Immunologic --- Diagnosis, Immunological --- Immunodiagnosis --- Diagnoses, Immunologic --- Diagnoses, Immunological --- Immunodiagnoses --- Immunologic Diagnoses --- Immunological Diagnoses --- Immunological Diagnosis --- Immunological Test --- Test, Immunologic --- Test, Immunological --- Tests, Immunological --- Arts, Industrial --- Industrial Arts --- diagnosis --- methods --- Lymphocyte Subsets --- Immunity --- Immunochemistry --- Occupations --- Cloud Computing --- Social medicine. --- Biotechnology. --- SCIENCE, TECHNOLOGY & SOCIETY/General --- BIOMEDICAL SCIENCES/General --- Chemical engineering --- Genetic engineering --- Medical care --- Medical sociology --- Medicine --- Medicine, Social --- Public health --- Public welfare --- Medical ethics --- Medical sociologists --- Social aspects --- Biological Markers. --- Immunophenotyping. --- Sociology, Medical.
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