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Cet ouvrage cherche à décrire de manière pédagogique l'utilisation des techniques par immunoanalyse. Il compte deux grandes parties complémentaires associant les domaines fondamental et appliqué. Au plan fondamental, les deux premiers chapitres regroupent les principes de base, les partenaires et les conditions expérimentales des immunodosages avec marqueurs. Au plan appliqué, les autres chapitres permettent de déterminer pout tout biologiste futur ou confirmé la façon de générer des résultats biologiques de qualité, en accord avec une réglementation de plus en plus documentée. Ce livre représente actuellement le seul ouvrage en français traitant des principes, des techniques et des applications biologiques de l'immunoanalyse quantitative avec marqueurs. Il souhaite apporter au lecteur le complément scientifique nécessaire pour répondre aux exigences de la nouvelle réforme de biologie proposant notamment de renforcer le caractère médical de la discipline et les compétences des professionnels de santé.

Immunology. --- Immunobiology --- Life sciences --- Serology

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Over the past several years, a high diversity of regulatory cells and suppressive molecules has taken centre stage in the field of immunoregulation. In Suppression and Regulation of Immune Responses: Methods and Protocols, expert researchers highlight recent advances in the identification, characterization, and generation of regulatory cells not only of the T cell lineage but also of other origins such as B, NK, myeloid, and dendritic cells, as well as the role of several suppressive molecules in immunoregulation. Particular emphasis is placed on the characterization of the molecular mechanisms and the therapeutic applications of regulatory cells and molecules in human diseases. Written as a volume in the highly successful Methods in Molecular Biology™ series, this work provides the kind of detailed description and implementation advice that is crucial for getting optimal results. Comprehensive and cutting-edge, Suppression and Regulation of Immune Responses: Methods and Protocols serves as a key reference for scientists seeking a way toward greater control over the enormous power of the complex and vital immune system.

Immunology. --- Immunobiology --- Life sciences --- Serology

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Over the last half century, a dramatic increase in allergic diseases has been observed throughout industrialized nations, which has resulted in significant worldwide socio-economic challenges. In Mouse Models of Allergic Disease: Methods and Protocols, a wide range of expert contributors provide detailed protocols for the design and execution of experiments to thoroughly analyze critical elements associated with a diverse range of allergic diseases, all through the lens of mouse models that accurately recapitulate clinically relevant aspects of the respective human disease.  The volume opens with a section featuring techniques essential for effective ex vivo cell isolation and evaluation of specific cell types relevant to a diverse range of allergic diseases, and the book then moves on to cover in vivo protocols to evaluate prevalent mouse models of human allergic diseases, including mouse models of systemic anaphylaxis, contact hypersensitivity, allergic rhinitis, and asthma, as well as a collection of chapters on in vivo and ex vivo protocols used to assess indirect mediators of allergic diseases, such as the nervous system, non-hematopoietic cells, and the composition of the gut microbiome.  Written in the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Timely and authoritative, Mouse Models of Allergic Disease: Methods and Protocols serves as an essential collection of protocols that allow both novice and expert researchers the ability to accurately develop, evaluate, and characterize the mechanisms associated with these disorders.

Immunology. --- Immunobiology --- Life sciences --- Serology

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At the intersection of experimental and computational sciences, the second edition of Immunoinformatics provides biological insights as well as a simpler way to implement approaches and algorithms in the immunoinformatics research domain. After an introductory section, this extensive volume moves on to cover topics such as databases, tools for prediction, systems biology approaches, as well as a variety of immunoinformatics applications.  As part of the highly successful Methods in Molecular Biology series, chapters include the type of detailed information and implementation advice to ensure successful results.   Comprehensive and practical, Immunoinformatics, Second Edition aims at students and researchers from diverse backgrounds and levels interested in working with immunological problems.

Immunology. --- Immunobiology --- Life sciences --- Serology

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In Basophils and Mast Cells: Methods and Protocols, experts in this challenging field explore techniques to research these cells from the most practical point of view. Given the tremendous influence of mast cells and blood-borne basophils over immune system function, this volume intends to aid the reader in the development of better tools for the isolation of these cells from primary tissues, peripheral blood, bone marrow, or cord blood. Also covered are protocols for the in vitro and in vivo study of their functions. Written in the highly successful Methods in Molecular Biology format, chapters in this book contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls straight from the researchers who use the methods most. Authoritative and easy to use, Basophils and Mast Cells: Methods and Protocols will provide the necessary tools for future research into mast cells and basophils with the goal of aiding in the quest to shed more light on these fascinating cell types.

Immunology. --- Immunobiology --- Life sciences --- Serology