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Analytical biochemistry --- Plant genetics. Plant evolution --- Plant molecular biology --- Plant molecular genetics --- Biologie moléculaire végétale --- Génétique moléculaire végétale --- Laboratory manuals --- Manuels de laboratoire --- Laboratory manuals. --- Biologie moléculaire végétale --- Génétique moléculaire végétale --- Plant molecular biology - Laboratory manuals. --- Plant molecular genetics - Laboratory manuals. --- Plant molecular biology. Laboratory manuals --- Plant molecular genetics. Laboratory manuals

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Yeast fungi --- Genetics --- Laboratory manuals --- 575.08 --- 582.282.232 --- Yeasts --- Fungi --- Basidiomycetes --- Blastomycetes --- Endomycetales --- 582.282.232 Sacharomycetaceae --- Sacharomycetaceae --- Genetics&delete& --- General genetics. General cytogenetics. Immunogenetics. Evolution. Speciation. Phylogeny--Biological techniques. Experimental methods. Equipment --- Yeast fungi - Genetics - Laboratory manuals

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Offering detailed protocols for those needing to construct a variety of maps and isolate genes, this unique book is intended to popularize the new techniques of genome analysis derived from the Human Genome Project. The power of these new methods is often most striking when applied to problems outside of human genetics, particularly the nonmammalian systems on which many researchers focus. Many of these organisms are economically important and biologically rich.Nonmammalian Genomic Analysis: A Practical Guide covers the ""how to"" aspects of preparation, handling, cloning, and analy

Gene mapping. --- Gene mapping --Laboratory manuals. Microbial genetics --Laboratory manuals. Plant genetics --Laboratory manuals. --- Laboratory manuals. --- Microbial genetics. --- Plant genetics. --- Gene mapping --- Microbial genetics --- Plant genetics --- Genetic Techniques --- Cytological Techniques --- Eukaryota --- Investigative Techniques --- Organisms --- Clinical Laboratory Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Cytogenetic Analysis --- Chromosome Mapping --- Plants --- Biology --- Health & Biological Sciences --- Genetics --- Laboratory manuals --- Microorganisms --- Chromosome mapping --- Genetic mapping --- Genome mapping --- Linkage mapping (Genetics) --- Mapping, Gene --- Microbiology --- Technique

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Myeloproliferative neoplasms (MPNs) are clonal stem cell diseases that, under the World Health Organization classification, are subcategorized into eight clinicopathologic entities. The current book focuses on three of these categories: polycythemia vera, essential thrombocythemia, and primary myelofibrosis. A number of important questions regarding the diagnosis, prognosis, and treatment of these diseases have arisen following the discovery of the JAK2 V617F mutation in the majority of patients with bcr/abl-negative MPNs.   This book comprises evidence-based reviews and expert consensus results. It offers comprehensive coverage of relevant laboratory and clinical advances and places great emphasis on the practical issues that challenge physicians in their daily practice. The main topics considered thus include contemporary diagnostic  approaches, the value and limitations of mutation screening for diagnostic and  prognostic purposes, risk stratification in terms of both survival and other  disease complications such as leukemic transformation and thrombosis, and  modern therapeutic strategies, including conventional drugs, allogeneic stem cell  transplantation, and experimental drugs still under study. The reader will find Critical Concepts and Management Recommendations in Myeloproliferative Neoplasms to be an invaluable and up-to-date source of information from leading authorities in the field.

Neoplasms -- genetics -- Laboratory Manuals. --- Neoplasms. --- Hematologic Neoplasms --- Bone Marrow Diseases --- Hematologic Diseases --- Neoplasms by Site --- Hemic and Lymphatic Diseases --- Neoplasms --- Diseases --- Bone Marrow Neoplasms --- Myeloproliferative Disorders --- Medicine --- Health & Biological Sciences --- Pathology --- Myeloproliferative disorders. --- Bone marrow --- Tumors. --- Myeloproliferative syndrome --- Medicine. --- Internal medicine. --- Hematology. --- Oncology. --- Medicine & Public Health. --- Internal Medicine. --- Oncology  . --- Tumors --- Medicine, Internal --- Haematology --- Internal medicine --- Blood

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This laboratory manual gives a thorough introduction to modern, cost efficient techniques in Molecular Biology, which can find application in many different fields. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses and workshops or tested in the author's laboratory. Thus, they are also likely to work in inexperienced hands the first time they are performed.

Step-by-step protocols and practical notes are provided. The distinguishing feature of this manual compared to others is the detailed explanation of the theoretical mechanisms of each step and the discussion of the importance of the techniques. In addition, each protocol includes an indication of the time and expense involved in its application. This knowledge will enable the users to design their own modifications or to adapt the method to different systems.

Dr. Surzycki has been teaching undergraduate courses and leading workshops in introductory Molecular Biology for many years, during which time he has extensively modified and refined the techniques he has described in this manual.

Keywords: DNA - DNA Isolierung - DNA sequencing - Nucleic acids - Nukleinsäuren - PCR - Polymerasekettenreaktion - RNA - Seuqenzierung - Southern Blot - biology - cloning - hybridization - molecular biology - preparation


General biochemistry --- polymers --- biotechnologie --- biochemie --- Biotechnology --- Molecular biology --- moleculaire biologie --- polymeren --- Macromolecules --- Molecular genetics --- Biochemistry --- Genetics --- Chemistry --- Biology --- Biological Science Disciplines --- Natural Science Disciplines --- Disciplines and Occupations --- Molecular Biology --- Health & Biological Sciences --- Biophysics --- klonen --- electrophoresis --- PCR (polymerase chain reaction) --- Biology—Technique. --- Biochemistry. --- Medicine. --- Biotechnology. --- Plant genetics. --- Biological Techniques. --- Biochemistry, general. --- Biomedicine, general. --- Plant Genetics and Genomics. --- Plants --- Chemical engineering --- Genetic engineering --- Health Workforce --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Medical sciences --- Composition --- Molecular biology - Laboratory manuals. --- Molecular genetics - Laboratory manuals.