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Synthetic biology is at such an early stage of development that there is no uniform agreement as yet about what it actually is. To some, it represents a natural extension of genetic engineering, and therefore is “business as usual”. For others, it is a way to bring mass manufacturing out from the decades of biotechnology research. Currently the discipline is limited by the ability to synthesise DNA cost-effectively but this is a technical barrier that it is anticipated will be overcome. Synthetic biology raises a number of policy issues around R&D funding, company investment, PPP arrangements and innovative financing, infrastructure requirements, education and training, intellectual property (IP), regulation, and public engagement. In preparation for the continuing development and greater use of synthetic biology, some countries have started to prepare synthetic biology technical roadmaps and a global roadmap for the medium term would be an extremely useful policy tool. Technical roadmaps could both identify likely future policy requirements, and be a useful vehicle in public engagement.
Biological systems. --- Genes, Synthetic. --- Genetic Engineering -- methods. --- Genetic engineering. --- Models, Genetic. --- Mechanical Engineering --- Engineering & Applied Sciences --- Bioengineering --- Synthetic biology. --- Bioengineering. --- Biological engineering --- Life science engineering --- Biology --- Engineering --- Synthetic biology --- Biotechnology
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Genetic Engineering --- Plasmids --- Genetics, Microbial --- methods --- 579.252.5 --- 57.083 --- 57.083 Microbiological, virological, immunological methods and techniques --- Microbiological, virological, immunological methods and techniques --- 579.252.5 Extrachromosomal hereditary determinants. Episomes. Plasmide. --- Extrachromosomal hereditary determinants. Episomes. Plasmide. --- Biological techniques --- Monera --- methodology --- Microbiology --- plasmids --- Biotechnology --- ADN --- DNA --- Electrophoresis --- Microscopy --- genetic transformation --- genes --- Transcription --- transcription --- genetic engineering --- Addresses, essays, lectures --- DNA. --- transcription. --- Molecular cloning. --- Genetic Engineering - methods --- Base sequence --- Nuclease, restriction endodeoxyribo --- -Plasmids --- Vecteur de clonage --- Translation --- Hybridation de dna
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Plasmids --- Genetic engineering --- Plasmides --- Génie génétique --- Research --- Methodology --- Recherche --- Méthodologie --- Genetic Engineering --- Genetics, Microbial --- methods --- Génie génétique --- Méthodologie --- Plasmids. --- Episome --- Plasmid --- Episomes --- DNA Transposable Elements --- Virophages --- #WSCH:MONO --- 579.25 --- 579.25 Microbial genetics --- Microbial genetics --- Microbial Genetics --- Genetic, Microbial --- Microbial Genetic --- Virus Physiological Phenomena --- Bacterial Physiological Phenomena --- Genome, Microbial --- Paragenes --- Cytoplasmic inheritance --- Genetic vectors --- Mobile genetic elements --- Extrachromosomal DNA --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Research&delete& --- General microbiology --- Genetic Engineering - methods --- Plasmids - Research - Methodology
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In recent years our ability to understand and manipulate epidermal cells has increased tremendously, opening significant new possibilities in both basic science research and in regenerative medicine, including wound healing and transplantation. In Epidermal Cells: Methods and Protocols, a panel of experienced basic and clinical researchers describe in step-by-step detail the powerful methods they have developed and optimized to analyze and manipulate epidermal cell precursors and mature epidermal cells. These protocols cover different methods and models for culturing epidermal cells, for enriching very early epidermal progenitors, as well as for studying epidermal cell commitment and differentiation both in vitro and in vivo. Topics of special interest include the derivation, characterization, and utility of epidermal stem cells, mature epidermal cells and their characterization, and applications in regenerative medicine. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Epidermal Cells: Methods and Protocols offers experienced and novice investigators alike an invaluable collection of readily reproducible techniques designed to broaden not only our understanding of the biology of epidermal cells, but also their utility in normal tissue homeostasis and regenerative medicine applications.
Epidermis --- Cell Culture Techniques --- Cytological Techniques --- Genetic Engineering --- Keratinocytes --- Organ Culture Techniques --- Physiology --- Research --- Methodology. --- Cytology --- cytology. --- methods. --- Cell Culture --- Organ Culture --- Methodology --- cytology --- methods --- Electronic books. -- local. --- Epidermis -- Cytology -- Research -- Methodology. --- Epidermis -- Physiology -- Research -- Methodology. --- Methods --- Epithelial Cells --- Epithelium --- Culture Techniques --- Investigative Techniques --- Clinical Laboratory Techniques --- Genetic Techniques --- Skin --- Cells --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Tissues --- Integumentary System --- Anatomy --- Human Anatomy & Physiology --- Health & Biological Sciences --- Internal medicine. --- Cytology. --- Internal Medicine. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cytologists --- Medicine, Internal --- Medicine --- Epidermis - Physiology - Research - Methodology --- Epidermis - Cytology - Research - Methodology --- Epidermis - cytology --- Cell Culture - methods --- Cytological Techniques - methods --- Genetic Engineering - methods --- Keratinocytes - cytology --- Organ Culture - methods --- Acqui 2006
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Molecular biology --- Analytical biochemistry --- DNA Polymerases --- Gene Amplification --- Genetic Engineering --- RNA Polymerases --- Polymerase chain reaction --- Gene amplification --- Réaction en chaîne de la polymérase --- Redondance génétique --- methods --- DNA-Directed RNA Polymerases --- Biologie moléculaire --- Enzymes --- Clonage moléculaire --- molecular cloning --- Virologie --- Virology --- Virus --- viruses --- Identification --- identification --- ADN --- DNA --- ARN --- RNA --- Génétique --- genetics --- Évolution --- evolution --- Expérimentation en laboratoire --- Laboratory experimentation --- Méthodologie --- methodology --- DNA-Directed DNA Polymerase. --- Nucleic Acid Amplification Techniques. --- DNA-Directed RNA Polymerases. --- 577.212.3 --- 57.088.1 --- Chain reaction, Polymerase --- PCR (Biochemistry) --- Polymerization --- DNA polymerases --- Amplification, Gene --- Gene expression --- Amplification Technics, Nucleic Acid --- Amplification Techniques, Nucleic Acid --- DNA Amplification Technics --- Nucleic Acid Amplification Technics --- Nucleic Acid Amplification Test --- Nucleic Acid Amplification Tests --- RNA Amplification Technics --- Technics, Nucleic Acid Amplification --- Techniques, Nucleic Acid Amplification --- DNA Amplification Techniques --- RNA Amplification Techniques --- Amplification Technic, DNA --- Amplification Technic, RNA --- Amplification Technics, DNA --- Amplification Technics, RNA --- Amplification Technique, DNA --- Amplification Technique, RNA --- Amplification Techniques, DNA --- Amplification Techniques, RNA --- DNA Amplification Technic --- DNA Amplification Technique --- RNA Amplification Technic --- RNA Amplification Technique --- Technic, DNA Amplification --- Technic, RNA Amplification --- Technics, DNA Amplification --- Technics, RNA Amplification --- Technique, DNA Amplification --- Technique, RNA Amplification --- Techniques, DNA Amplification --- Techniques, RNA Amplification --- methods. --- Nucleic acid base and sequence compositon. Experimental deciphering of genetic code. --- Methods for analysis and estimation --- Polymerase chain reaction. --- Gene amplification. --- DNA Polymerases. --- RNA Polymerases. --- 57.088.1 Methods for analysis and estimation --- 577.212.3 Nucleic acid base and sequence compositon. Experimental deciphering of genetic code. --- Dna polymerases. --- Genetic engineering --- Rna polymerases. --- Methods. --- Réaction en chaîne de la polymérase --- Redondance génétique --- DNA-Directed DNA Polymerase --- Nucleic Acid Amplification Techniques --- DNA-Directed RNA Polymerase --- RNA Polymerase --- Transcriptase --- DNA-Dependent RNA Polymerases --- Transcriptases --- DNA Dependent RNA Polymerases --- DNA Directed RNA Polymerase --- DNA Directed RNA Polymerases --- Polymerase, DNA-Directed RNA --- Polymerase, RNA --- Polymerases, DNA-Dependent RNA --- Polymerases, DNA-Directed RNA --- Polymerases, RNA --- RNA Polymerase, DNA-Directed --- RNA Polymerases, DNA-Dependent --- RNA Polymerases, DNA-Directed --- DNA Polymerase N3 --- DNA-Dependent DNA Polymerases --- DNA Dependent DNA Polymerases --- DNA Directed DNA Polymerase --- DNA Polymerase, DNA-Directed --- DNA Polymerases, DNA-Dependent --- Polymerase N3, DNA --- Polymerase, DNA-Directed DNA --- Polymerases, DNA --- Polymerases, DNA-Dependent DNA --- Nucleic acid base and sequence compositon. Experimental deciphering of genetic code --- viruses. --- identification. --- DNA. --- RNA. --- evolution. --- Nucleic Acid Amplification Technic --- Nucleic Acid Amplification Technique --- DNA Polymerase --- Polymerase, DNA --- Gene Amplification - methods --- Genetic Engineering - methods --- Polymerases --- Rna --- DNA POLYMERASES --- GENE AMPLIFICATION --- GENETIC ENGINEERING --- RNA POLYMERASES --- METHODS
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