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Cardiac Gene Expression: Methods and Protocols presents both cutting-edge and established methods for studying cardiac gene expression. The protocols provide a template for solid research, and cover the process through screening, analysis, characterization, and functional confirmation of novel genes or known genes with a new function. Section I, Cardiac Gene Expression Profiling: The Global Perspective, discusses several different approaches to examining, identifying, and analyzing changes in transcriptome gene expression. Section II, Cardiac Gene Regulation: Gene-Specific mRNA Measurement in the Myocardium, outlines more sensitive and gene-targeted expression methods. Section III, Cardiac Gene Regulation: Promoter Characterization in the Myocardium, provides protocols for the study of underlying gene regulation mechanisms by focusing on the interaction of transcription factors with their cognate cis binding elements. Section IV, In Silico Assessment of Regulatory cis-Elements and Gene Regulation, and Section V, Cardiac Single Network Polymorphisms, emphasize new analytical approaches for deciphering the functional elements buried in the 3 billion nucleotides of the human genome and other model genomes. The concluding section, Gene Overexpression and Targeting in the Myocardium, highlights methods that facilitate overexpression or cardiac-specific targeted gene deletion.

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In Post-Transcriptional Gene Regulation, renowned authors present current technical approaches to most aspects of post-transcriptional control and provide a useful and versatile laboratory bench resource. With chapters split into sections covering bioinformatics, fundamental aspects of the study of RNA biology, and techniques for specific aspects of RNA biology, the expert authors have filled the book with invaluable tricks of the trade, perfected in their state-of-the-art laboratories. This new volume from the Methods in Molecular Biology series is convinently divided into three sections. The first section presents a series of bioinformatic approaches to address the use of RNA databases and algorithms to the study of post-transcriptional regulation involving untranslated regions of transcripts. In the second section, a series of methods applicable to fundamental issues in mRNA biology are presented. These include RNA structure/function, mRNP analysis and novel methods for mRNA labeling and isolation. The third section of this volume presents methodologies to study particular aspects of post-transcriptional control. This section includes methods for the study of alternative splicing and 3’ end processing, mRNA localization, mRNA translation, mRNA stability and si/miRNA regulation. Collectively, Post-Transcriptional Gene Regulation provides the reader with a useful and versatile laboratory bench resource that will become an essential reference in the field.

Genetic regulation --- Messenger RNA --- Cellular control mechanisms --- Bioinformatics --- Gene Expression Regulation --- RNA Processing, Post-Transcriptional --- Computational Biology --- Bioinformatics. --- Régulation génétique --- ARN messager --- Régulation cellulaire --- Bio-informatique --- Laboratory manuals --- Manuels de laboratoire --- Informational RNA --- Messenger ribonucleic acid --- mRNA --- Protein transcript --- Protein transcripts --- Template RNA --- Bio-informatics --- Biological informatics --- Biology --- Information science --- Computational biology --- Systems biology --- Data processing --- Cell regulation --- Biological control systems --- Cell metabolism --- RNA --- Gene expression --- Gene expression regulation --- Gene regulation --- Biosynthesis --- Molecular genetics --- Regulation --- Biochemistry. --- Cytology. --- Human genetics. --- Biochemistry, general. --- Cell Biology. --- Human Genetics. --- Cell biology --- Cellular biology --- Cells --- Cytologists --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Composition --- Genetic regulation - Laboratory manuals --- Messenger RNA - Laboratory manuals --- Cellular control mechanisms - Laboratory manuals --- Gene Expression Regulation - laboratory manuals --- RNA Processing, Post-Transcriptional - Laboratory Manuals