Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Gene libraries --- Gene libraries --- Gene libraries

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Human gene libraries. --- Neoplasms --- Neoplasms --- Oncogenes --- Oncogenes --- Oncogenes --- Genetics --- Genetics

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Genetics, Medical --- Hereditary Diseases --- Phenotype --- Human gene libraries --- Human chromosomes --- Human chromosome abnormalities --- Catalogs and collections --- Human gene libraries. --- Catalogs and collections. --- bibliography. --- encyclopedias. --- Human chromosomes - Catalogs and collections --- Human chromosome abnormalities - Catalogs and collections --- Bibliography. --- Encyclopedias.

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Directed protein evolution-a powerful technique for the discovery of new enzymes and therapeutic proteins-has matured to the point that it is now the centerpiece of enzyme engineering, where it has catalyzed the development of numerous experimental methods. In Directed Evolution Library Creation: Methods and Protocols, seasoned practitioners from many leading laboratories share their best experimental protocols for the generation of molecular diversity. Described in step-by-step detail to ensure experimental success, these protocols include readily reproducible methods for random mutagenesis of entire genes or segments of genes, for homologous and nonhomologous recombination, and for constructing in vivo libraries in bacteria and yeast. In addition to the various protocols for creating libraries, this volume also describes ways to analyze libraries, particularly those made by recombination. An accompanying volume, Directed Enzyme Evolution: Screening and Selection Methods (ISBN: 1-58829-286-X), is devoted entirely to selection and screening methods that can be applied to the directed evolution of enzymes. Taken together, Directed Evolution Library Creation: Methods and Protocols and Directed Enzyme Evolution: Screening and Selection Methods capture for newcomers and more experienced investigators alike all the key methods for using directed protein evolution to better understand protein structure-function relationships, to discover new enzymes and therapeutic proteins, and to design new assays suitable for specific applications.

Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Gene libraries --- Genetic engineering --- Molecular evolution --- Microbial mutation --- Combinatorial chemistry --- Genetic recombination

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Chemical library technologies have brought about dramatic changes in the drug discovery process, and, though still evolving, they have become an integral part of ongoing drug discovery research. In Chemical Library Design, experts in the field provide methods and detailed protocols delving into this key process of selecting useful, biologically relevant compounds from large pools of synthesizable candidates. This compendium includes chapters on historical overviews, state-of-the-art methodologies, including structure-based and fragment-based library design, practical software tools, and successful and important applications of chemical library design. As a volume in the popular Methods in Molecular Biology™ series, the thorough contributions provide the kind of meticulous description and implementation advice that is crucial for getting optimal results. Authoritative and cutting-edge, Chemical Library Design is an ideal reference for all scientists seeking the technology needed to aid in the search for new and vital drugs.

Biochemistry. --- Gene libraries. --- Biochemistry, general. --- Combinatorial Libraries. --- Gene banks (Genetic engineering) --- Genomic banks --- Genomic libraries --- Biological resource centers --- Genomes --- Recombinant DNA --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition