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As our understanding of the biological sciences expands, the boundaries between traditional disciplines tend to blur at the edges. Physiologists and pharmacologists, for instance, now need to embrace techniques that until recently were the strict preserves of biochemists and molecular biologists. However, the acquisition of new technologies can be a time-consuming and frustrating business, and unless an expert is on hand to give instruction, precious hours can be spent poring over half-described Methods sections with no guarantee of eventual success. The aim of Signal Transduction Protocols has been to get experts with "hands-on" experience in particular techniques to give detailed accounts of experimental protocols in a recipe-type format, which we hope will circumvent the problems of ambiguity often encountered when reading the literature. The techniques described in Signal Transduction Protocols are those that we think will be most useful in addressing questions in the area of receptor-mediated cell signaling, with particular regard to those receptors that are part of the G-protein-linked superfamily. To keep it to a manageable size, we have omitted any reference to electrophysiology and have instead concentrated on more biochemical approaches.

Molecular biology --- Cellular signal transduction --- Cell receptors --- G proteins --- Research --- Methodology --- 577.2 --- 547 --- -Cellular signal transduction --- -G proteins --- -#WDIR:vfm2 --- GTP-binding proteins --- GTP regulatory proteins --- Guanine nucleotide-binding proteins --- Guanine nucleotide regulatory proteins --- Membrane proteins --- Cellular information transduction --- Information transduction, Cellular --- Signal transduction, Cellular --- Bioenergetics --- Cellular control mechanisms --- Information theory in biology --- Cell membrane receptors --- Cell surface receptors --- Receptors, Cell --- Binding sites (Biochemistry) --- Cell membranes --- Proteins --- Molecular bases of life. Molecular biology --- Organic chemistry --- -Methodology --- Methodology. --- 547 Organic chemistry --- 577.2 Molecular bases of life. Molecular biology --- #WDIR:vfm2 --- Research&delete& --- Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Cellular signal transduction - Research - Methodology --- Cell receptors - Research - Methodology --- G proteins - Research - Methodology