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Immunoassay techniques have become essential in various fields of pure and applied research. This volume of the well known Laboratory Techniques series will be of assistance to those who have plans or are making efforts to develop ultrasensitive enzyme immunoassays for antigens and antibodies. The volume describes factors limiting the sensitivity of noncompetitive solid phase enzyme immunoassays, methods to overcome difficulties limiting sensitivities, methods to perform ultrasensitive enzyme immunoassays as rapidly as possible, and protocols of enzyme labeling and enzyme assays as
Enzymes --- Enzyme activity --- antibodies --- Immunoenzyme techniques --- Enzyme-linked immunosorbent assay --- Molecular biology --- Technique. --- ELISA --- Enzyme immunoassay --- Enzyme labeled antibody technique --- Enzyme labeled immunoassay --- Enzyme linked immunoassay --- Enzyme linked immunospecific assay --- Immunoassay, Enzyme --- Immunoenzymometric assay --- Immunoenzyme technique --- Solid-phase analysis
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Electronic books. -- local. --- Enzyme-linked immunosorbent assay. --- Immunoenzyme technique. --- Enzyme-linked immunosorbent assay --- Enzyme-Linked Immunosorbent Assay --- Immunoenzyme Techniques --- Immunosorbent Techniques --- Immunoassay --- Immunohistochemistry --- Molecular Probe Techniques --- Immunologic Tests --- Immunologic Techniques --- Investigative Techniques --- Laboratory Techniques and Procedures --- Diagnosis --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Animal Biochemistry --- Human Anatomy & Physiology --- Health & Biological Sciences --- Moleculaire biologie --- Moleculaire biologie. --- Antibody enzyme techniques, Unlabeled --- Immunoperoxidase technique --- Peroxidase labeled antibody technique --- Enzymatic analysis --- Immunology --- Protein binding --- ELISA --- Enzyme immunoassay --- Enzyme labeled antibody technique --- Enzyme labeled immunoassay --- Enzyme linked immunoassay --- Enzyme linked immunospecific assay --- Immunoassay, Enzyme --- Immunoenzymometric assay --- Immunoenzyme technique --- Solid-phase analysis --- Technique --- Immunology. --- Immunobiology --- Life sciences --- Serology
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This book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It also reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters. The book guides readers through the respective steps of the analytical assay, while also familiarizing them with the possible sources of error in the assay. It offers detailed insights into the immobilization techniques used for protein attachment, as well as methods for evaluating the assay and calculating the key parameters, such as sensitivity, specificity, accuracy and limit of detection. In addition, the book explores the advantages and shortcomings of the conventional ELISA, as well as various approaches to improving its performance. In this regard, merging and integrating other technologies with widely known ELISAs have opened new avenues for the advancement of this immunoassay. Accordingly, the book provides cutting-edge information on integrated platforms such as ELISpot, plasmonic ELISAs, sphere-/bead-based ELISAs, paper-/fiber-based ELISAs and ELISA in micro-devices. .
Enzyme-linked immunosorbent assay. --- Biomedical engineering. --- Immunology. --- RNA-ligand interactions. --- Biotechnology. --- Bioinformatics. --- Biomedical Engineering. --- Biological and Medical Physics, Biophysics. --- Protein-Ligand Interactions. --- Computational Biology/Bioinformatics. --- ELISA --- Enzyme immunoassay --- Enzyme labeled antibody technique --- Enzyme labeled immunoassay --- Enzyme linked immunoassay --- Enzyme linked immunospecific assay --- Immunoassay, Enzyme --- Immunoenzymometric assay --- Immunoenzyme technique --- Solid-phase analysis --- Biomedical Engineering and Bioengineering. --- Bio-informatics --- Biological informatics --- Biology --- Information science --- Computational biology --- Systems biology --- Chemical engineering --- Genetic engineering --- Immunobiology --- Life sciences --- Serology --- Clinical engineering --- Medical engineering --- Bioengineering --- Biophysics --- Engineering --- Medicine --- Data processing --- Biophysics. --- Biological physics. --- Proteins . --- Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Biological physics --- Medical sciences --- Physics
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Wild animals under human care as well as domesticated farm production animals are often exposed to environmental changes (e.g., capture and transportation). Short-term or acute changes in physiological indices (e.g., heart rate, respiration, body temperatures, immune cells, and stress hormonal biomarkers) provide crucial information regarding the responses of animals to novel environments, and they could provide crucial determining factors for the long-term health and welfare of animals. This Special Issue includes experimental research papers that demonstrate the applications of physiological indices and welfare assessment methods (e.g., morphological and morphometric data, behavioural assessments, thermal profiles, and physiological markers) in any wildlife or production animal (e.g., rescued and rehabilitating animals, pets, competition animals, farm animals, and zoo animals), in response to environmental and management related factors. The goal is to provide examples of new research and techniques that can be used to monitor short- and long-term environmental adaptation of animals under human care.
Research & information: general --- Biology, life sciences --- Zoology & animal sciences --- non-invasive --- bioindicator --- pollution --- stress --- welfare --- constructed wetland --- glucocorticoid --- urban river --- reindeer --- glucocorticoids --- validation --- rodents --- ecological immunology --- natural antibodies --- haptoglobin --- neutrophil to lymphocyte ratio --- immune strategy --- vector-borne pathogens --- parasitology --- zoonosis --- thermal imaging --- koalas --- body temperature --- heat/cold stress --- thermoregulation --- substrate --- Zoo --- protocol --- veterinary assessment --- Punjab urial --- body condition --- behavior --- Pakistan --- red deer --- hind --- reproduction --- progesterone --- cortisol --- hair --- Asian elephant --- saliva --- immunoglobulin A --- circadian rhythm --- Panthera tigris tigris --- Panthera tigris altaica --- siberian --- tigers --- bengal tigers --- captive --- biochemical parameter --- serum protein electrophoresis --- Cervus elaphus --- plasma --- feces --- hunting --- zoo --- faecal analysis --- glucocorticoid metabolites --- enzyme immunoassay --- roe deer --- blood parameters --- prognostic factors --- blood lactate concentration --- biomarkers --- captivity-induced stress --- fecal glucocorticoid metabolites --- physiological stress in rodents --- oxytocin --- boar --- ejaculation --- wildlife --- environmental stress --- urbanisation --- birds --- non-invasive --- bioindicator --- pollution --- stress --- welfare --- constructed wetland --- glucocorticoid --- urban river --- reindeer --- glucocorticoids --- validation --- rodents --- ecological immunology --- natural antibodies --- haptoglobin --- neutrophil to lymphocyte ratio --- immune strategy --- vector-borne pathogens --- parasitology --- zoonosis --- thermal imaging --- koalas --- body temperature --- heat/cold stress --- thermoregulation --- substrate --- Zoo --- protocol --- veterinary assessment --- Punjab urial --- body condition --- behavior --- Pakistan --- red deer --- hind --- reproduction --- progesterone --- cortisol --- hair --- Asian elephant --- saliva --- immunoglobulin A --- circadian rhythm --- Panthera tigris tigris --- Panthera tigris altaica --- siberian --- tigers --- bengal tigers --- captive --- biochemical parameter --- serum protein electrophoresis --- Cervus elaphus --- plasma --- feces --- hunting --- zoo --- faecal analysis --- glucocorticoid metabolites --- enzyme immunoassay --- roe deer --- blood parameters --- prognostic factors --- blood lactate concentration --- biomarkers --- captivity-induced stress --- fecal glucocorticoid metabolites --- physiological stress in rodents --- oxytocin --- boar --- ejaculation --- wildlife --- environmental stress --- urbanisation --- birds
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Enzyme-linked immunosorbent assay --- -Pathogenic microorganisms --- -Veterinary clinical pathology --- -Veterinary medicine --- -57.083.3 --- 57.083.3 Immunological methods and techniques --- Immunological methods and techniques --- Farriery --- Large animal medicine --- Large animal veterinary medicine --- Livestock medicine --- Veterinary science --- Medicine --- Animal health --- Animals --- Domestic animals --- Livestock --- Clinical pathology, Veterinary --- Veterinary medicine --- Diagnosis, Laboratory --- Veterinary pathology --- Disease-causing microorganisms --- Micro-organisms, Pathogenic --- Pathogens --- Microorganisms --- Medical microbiology --- Virulence (Microbiology) --- ELISA --- Enzyme immunoassay --- Enzyme labeled antibody technique --- Enzyme labeled immunoassay --- Enzyme linked immunoassay --- Enzyme linked immunospecific assay --- Immunoassay, Enzyme --- Immunoenzymometric assay --- Immunoenzyme technique --- Solid-phase analysis --- Congresses --- Identification --- -Congresses --- Diagnosis --- Diseases --- Losses --- Veterinary clinical pathology --- Pathogenic microorganisms --- Veterinary microbiology --- 57.083.3 --- Agricultural microbiology --- Communicable diseases in animals --- Identification&delete& --- Diagnosis&delete& --- Technique&delete& --- Congresses. --- Technique
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The purpose of this Special Issue “Cow’s Milk and Allergy” is to provide an overview of the association of cow’s milk with allergy. This topic has two quite different faces. On the one hand, we are all aware of the importance of cow’s milk allergy in early life. What is less known is that the consumption of raw, unprocessed milk is associated with a lower incidence of asthma and rhinitis. This Special Issue takes a closer look at all of these aspects of cow’s milk and allergy and focus on the following questions:
animal models --- milk allergen --- allergenicity --- camel milk --- immune regulation --- epigenetics --- human breast milk --- milk processing --- CAP-FEIA (Fluorescence Enzyme Immunoassay) --- immune system --- raw milk --- multiplex dot test --- Middle-East --- alkaline phosphatase --- cow’s milk allergy --- polygenic risk score --- tolerance --- hydrolysate --- CML --- infant formula --- miRNA-150 --- milk --- allergy --- casein --- asthma --- partial hydrolysate --- Immunoglubuline E --- molecular diagnosis --- allergic march --- delayed-type hypersensitivity --- allergenicity modulation --- prebiotics --- processing --- sRAGE --- histone acetylation --- dietary intervention --- probiotics --- cow’s milk --- Cow’s milk allergy (CMA) --- cow’s milk protein allergy --- farming effect --- protection --- anaphylaxis --- aggregation --- IgE binding --- natural history --- epidemiology --- prevalence --- infant allergy --- basophil activation test (BAT) --- ?-lactoglobulin --- step-down --- sensitization pattern --- extracellular vesicles --- cell-mediated reactions --- proteomics --- prevention of cow’s milk allergy --- therapy --- raw cow’s milk --- baked milk --- immune cells --- food allergy --- gut microbiota --- allergic diseases --- glycation --- bioactive peptides --- infant feeding --- cow’s milk allergens --- treatment of cow’s milk allergy --- formula --- genome-wide association --- diagnosis of cow’s milk allergy
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Wild animals under human care as well as domesticated farm production animals are often exposed to environmental changes (e.g., capture and transportation). Short-term or acute changes in physiological indices (e.g., heart rate, respiration, body temperatures, immune cells, and stress hormonal biomarkers) provide crucial information regarding the responses of animals to novel environments, and they could provide crucial determining factors for the long-term health and welfare of animals. This Special Issue includes experimental research papers that demonstrate the applications of physiological indices and welfare assessment methods (e.g., morphological and morphometric data, behavioural assessments, thermal profiles, and physiological markers) in any wildlife or production animal (e.g., rescued and rehabilitating animals, pets, competition animals, farm animals, and zoo animals), in response to environmental and management related factors. The goal is to provide examples of new research and techniques that can be used to monitor short- and long-term environmental adaptation of animals under human care.
non-invasive --- bioindicator --- pollution --- stress --- welfare --- constructed wetland --- glucocorticoid --- urban river --- reindeer --- glucocorticoids --- validation --- rodents --- ecological immunology --- natural antibodies --- haptoglobin --- neutrophil to lymphocyte ratio --- immune strategy --- vector-borne pathogens --- parasitology --- zoonosis --- thermal imaging --- koalas --- body temperature --- heat/cold stress --- thermoregulation --- substrate --- Zoo --- protocol --- veterinary assessment --- Punjab urial --- body condition --- behavior --- Pakistan --- red deer --- hind --- reproduction --- progesterone --- cortisol --- hair --- Asian elephant --- saliva --- immunoglobulin A --- circadian rhythm --- Panthera tigris tigris --- Panthera tigris altaica --- siberian --- tigers --- bengal tigers --- captive --- biochemical parameter --- serum protein electrophoresis --- Cervus elaphus --- plasma --- feces --- hunting --- zoo --- faecal analysis --- glucocorticoid metabolites --- enzyme immunoassay --- roe deer --- blood parameters --- prognostic factors --- blood lactate concentration --- biomarkers --- captivity-induced stress --- fecal glucocorticoid metabolites --- physiological stress in rodents --- oxytocin --- boar --- ejaculation --- wildlife --- environmental stress --- urbanisation --- birds --- n/a
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The application of analytical chemistry to the food sector allows the determination of the chemical composition of foods and the properties of their constituents, contributing to the definition of their nutritional and commodity value. Furthermore, it is possible to study the chemical modifications that food constituents undergo as a result of the treatments they undergo (food technology). Food analysis, therefore, allows us not only to determine the quality of a product or its nutritional value, but also to reveal adulterations and identify the presence of xenobiotic substances potentially harmful to human health. Furthermore, some foods, especially those of plant origin, contain numerous substances with beneficial effects on health. While these functional compounds can be obtained from a correct diet, they can also be extracted from food matrices for the formulation of nutraceutical products or added to foods by technological or biotechnological means for the production of functional foods. On the other hand, the enormous growth of the food industry over the last 50 years has broadened the field of application of analytical chemistry to encompass not only food but also food technology, which is fundamental for increasing the production of all types of food.
Humanities --- Social interaction --- solid-liquid extraction --- green extraction --- RSLDE --- bioactive compounds --- Naviglio extractor --- Naviglio’s principle --- Hsian-tsao --- Platostoma palustre (Blume) --- headspace solid-phase microextraction (SPME) --- volatile components --- simultaneous distillation-extraction (SDE) --- amino acid profiling --- hydrophilic interaction chromatography (HILIC) --- tandem mass spectrometry --- Triticum species flours --- flour quality characteristics --- narrow-leaved oleaster fruits --- near-infrared hyperspectral imaging --- geographical origin --- convolutional neural network --- effective wavelengths --- food colorants (synthetic, natural) --- food matrices --- instrumental analysis --- sample preparation --- mango --- volatile compounds --- frequency detection (FD) --- order-specific magnitude estimation (OSME) --- odor activity value --- sensory analysis --- lead (II) --- ELISA --- monoclonal antibody (mAb) --- isothiocyanobenzyl-EDTA (ITCBE) --- chemiluminescent enzyme immunoassay (CLEIA) --- meadow saffron --- metabolomics --- UHPLC-QTOF-mass spectrometry --- extraction methods --- antioxidants --- Pressurized liquid extraction --- soxhlet --- solvent extraction --- green analytical chemistry --- Rosemary --- poultry eggs --- spectinomycin --- lincomycin --- ASE --- GC-EI/MS/MS --- acrylamide --- kobbah --- transglutaminase --- pectin --- chitosan-nanoparticles --- coatings --- mesoporous silica nanoparticles --- grass pea --- HPLC-RP --- Curcuma longa L. --- curcuminoid stability --- multi-step extraction --- ultrasound-assisted extraction --- extraction kinetic --- functional foods --- gas chromatography --- health effects --- liquid chromatography (HPLC) --- mass spectrometry --- nutraceuticals --- phytochemicals --- solid-liquid extraction techniques --- solid-liquid extraction --- green extraction --- RSLDE --- bioactive compounds --- Naviglio extractor --- Naviglio’s principle --- Hsian-tsao --- Platostoma palustre (Blume) --- headspace solid-phase microextraction (SPME) --- volatile components --- simultaneous distillation-extraction (SDE) --- amino acid profiling --- hydrophilic interaction chromatography (HILIC) --- tandem mass spectrometry --- Triticum species flours --- flour quality characteristics --- narrow-leaved oleaster fruits --- near-infrared hyperspectral imaging --- geographical origin --- convolutional neural network --- effective wavelengths --- food colorants (synthetic, natural) --- food matrices --- instrumental analysis --- sample preparation --- mango --- volatile compounds --- frequency detection (FD) --- order-specific magnitude estimation (OSME) --- odor activity value --- sensory analysis --- lead (II) --- ELISA --- monoclonal antibody (mAb) --- isothiocyanobenzyl-EDTA (ITCBE) --- chemiluminescent enzyme immunoassay (CLEIA) --- meadow saffron --- metabolomics --- UHPLC-QTOF-mass spectrometry --- extraction methods --- antioxidants --- Pressurized liquid extraction --- soxhlet --- solvent extraction --- green analytical chemistry --- Rosemary --- poultry eggs --- spectinomycin --- lincomycin --- ASE --- GC-EI/MS/MS --- acrylamide --- kobbah --- transglutaminase --- pectin --- chitosan-nanoparticles --- coatings --- mesoporous silica nanoparticles --- grass pea --- HPLC-RP --- Curcuma longa L. --- curcuminoid stability --- multi-step extraction --- ultrasound-assisted extraction --- extraction kinetic --- functional foods --- gas chromatography --- health effects --- liquid chromatography (HPLC) --- mass spectrometry --- nutraceuticals --- phytochemicals --- solid-liquid extraction techniques
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The application of analytical chemistry to the food sector allows the determination of the chemical composition of foods and the properties of their constituents, contributing to the definition of their nutritional and commodity value. Furthermore, it is possible to study the chemical modifications that food constituents undergo as a result of the treatments they undergo (food technology). Food analysis, therefore, allows us not only to determine the quality of a product or its nutritional value, but also to reveal adulterations and identify the presence of xenobiotic substances potentially harmful to human health. Furthermore, some foods, especially those of plant origin, contain numerous substances with beneficial effects on health. While these functional compounds can be obtained from a correct diet, they can also be extracted from food matrices for the formulation of nutraceutical products or added to foods by technological or biotechnological means for the production of functional foods. On the other hand, the enormous growth of the food industry over the last 50 years has broadened the field of application of analytical chemistry to encompass not only food but also food technology, which is fundamental for increasing the production of all types of food.
Humanities --- Social interaction --- solid-liquid extraction --- green extraction --- RSLDE --- bioactive compounds --- Naviglio extractor --- Naviglio’s principle --- Hsian-tsao --- Platostoma palustre (Blume) --- headspace solid-phase microextraction (SPME) --- volatile components --- simultaneous distillation-extraction (SDE) --- amino acid profiling --- hydrophilic interaction chromatography (HILIC) --- tandem mass spectrometry --- Triticum species flours --- flour quality characteristics --- narrow-leaved oleaster fruits --- near-infrared hyperspectral imaging --- geographical origin --- convolutional neural network --- effective wavelengths --- food colorants (synthetic, natural) --- food matrices --- instrumental analysis --- sample preparation --- mango --- volatile compounds --- frequency detection (FD) --- order-specific magnitude estimation (OSME) --- odor activity value --- sensory analysis --- lead (II) --- ELISA --- monoclonal antibody (mAb) --- isothiocyanobenzyl-EDTA (ITCBE) --- chemiluminescent enzyme immunoassay (CLEIA) --- meadow saffron --- metabolomics --- UHPLC-QTOF-mass spectrometry --- extraction methods --- antioxidants --- Pressurized liquid extraction --- soxhlet --- solvent extraction --- green analytical chemistry --- Rosemary --- poultry eggs --- spectinomycin --- lincomycin --- ASE --- GC-EI/MS/MS --- acrylamide --- kobbah --- transglutaminase --- pectin --- chitosan-nanoparticles --- coatings --- mesoporous silica nanoparticles --- grass pea --- HPLC-RP --- Curcuma longa L. --- curcuminoid stability --- multi-step extraction --- ultrasound-assisted extraction --- extraction kinetic --- functional foods --- gas chromatography --- health effects --- liquid chromatography (HPLC) --- mass spectrometry --- nutraceuticals --- phytochemicals --- solid-liquid extraction techniques
Choose an application
The application of analytical chemistry to the food sector allows the determination of the chemical composition of foods and the properties of their constituents, contributing to the definition of their nutritional and commodity value. Furthermore, it is possible to study the chemical modifications that food constituents undergo as a result of the treatments they undergo (food technology). Food analysis, therefore, allows us not only to determine the quality of a product or its nutritional value, but also to reveal adulterations and identify the presence of xenobiotic substances potentially harmful to human health. Furthermore, some foods, especially those of plant origin, contain numerous substances with beneficial effects on health. While these functional compounds can be obtained from a correct diet, they can also be extracted from food matrices for the formulation of nutraceutical products or added to foods by technological or biotechnological means for the production of functional foods. On the other hand, the enormous growth of the food industry over the last 50 years has broadened the field of application of analytical chemistry to encompass not only food but also food technology, which is fundamental for increasing the production of all types of food.
solid-liquid extraction --- green extraction --- RSLDE --- bioactive compounds --- Naviglio extractor --- Naviglio’s principle --- Hsian-tsao --- Platostoma palustre (Blume) --- headspace solid-phase microextraction (SPME) --- volatile components --- simultaneous distillation-extraction (SDE) --- amino acid profiling --- hydrophilic interaction chromatography (HILIC) --- tandem mass spectrometry --- Triticum species flours --- flour quality characteristics --- narrow-leaved oleaster fruits --- near-infrared hyperspectral imaging --- geographical origin --- convolutional neural network --- effective wavelengths --- food colorants (synthetic, natural) --- food matrices --- instrumental analysis --- sample preparation --- mango --- volatile compounds --- frequency detection (FD) --- order-specific magnitude estimation (OSME) --- odor activity value --- sensory analysis --- lead (II) --- ELISA --- monoclonal antibody (mAb) --- isothiocyanobenzyl-EDTA (ITCBE) --- chemiluminescent enzyme immunoassay (CLEIA) --- meadow saffron --- metabolomics --- UHPLC-QTOF-mass spectrometry --- extraction methods --- antioxidants --- Pressurized liquid extraction --- soxhlet --- solvent extraction --- green analytical chemistry --- Rosemary --- poultry eggs --- spectinomycin --- lincomycin --- ASE --- GC-EI/MS/MS --- acrylamide --- kobbah --- transglutaminase --- pectin --- chitosan-nanoparticles --- coatings --- mesoporous silica nanoparticles --- grass pea --- HPLC-RP --- Curcuma longa L. --- curcuminoid stability --- multi-step extraction --- ultrasound-assisted extraction --- extraction kinetic --- functional foods --- gas chromatography --- health effects --- liquid chromatography (HPLC) --- mass spectrometry --- nutraceuticals --- phytochemicals --- solid-liquid extraction techniques
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