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Laboratory techniques in biochemistry and molecular biology
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ISBN: 0444502025 1281715859 9786611715854 0080858961 0444502017 9780444502018 Year: 1999 Publisher: Amsterdam Oxford North-Holland Pub. Co.

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Abstract

Immunoassay techniques have become essential in various fields of pure and applied research. This volume of the well known Laboratory Techniques series will be of assistance to those who have plans or are making efforts to develop ultrasensitive enzyme immunoassays for antigens and antibodies. The volume describes factors limiting the sensitivity of noncompetitive solid phase enzyme immunoassays, methods to overcome difficulties limiting sensitivities, methods to perform ultrasensitive enzyme immunoassays as rapidly as possible, and protocols of enzyme labeling and enzyme assays as

ELISA : theory and practice
Authors: ---
ISBN: 0896032795 9786610836512 1280836512 1592595294 9780896032798 Year: 1995 Publisher: Totowa, N.J. : Humana Press,


Book
Enzyme-linked Immunosorbent Assay (ELISA) : From A to Z
Authors: --- --- ---
ISBN: 981106766X 9811067651 Year: 2018 Publisher: Singapore : Springer Singapore : Imprint: Springer,

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This book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It also reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters. The book guides readers through the respective steps of the analytical assay, while also familiarizing them with the possible sources of error in the assay. It offers detailed insights into the immobilization techniques used for protein attachment, as well as methods for evaluating the assay and calculating the key parameters, such as sensitivity, specificity, accuracy and limit of detection. In addition, the book explores the advantages and shortcomings of the conventional ELISA, as well as various approaches to improving its performance. In this regard, merging and integrating other technologies with widely known ELISAs have opened new avenues for the advancement of this immunoassay. Accordingly, the book provides cutting-edge information on integrated platforms such as ELISpot, plasmonic ELISAs, sphere-/bead-based ELISAs, paper-/fiber-based ELISAs and ELISA in micro-devices. .


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Assessing the Environmental Adaptation of Wildlife and Production Animals : Applications of Physiological Indices and Welfare Assessment Tools
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Year: 2021 Publisher: Basel, Switzerland MDPI - Multidisciplinary Digital Publishing Institute

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Abstract

Wild animals under human care as well as domesticated farm production animals are often exposed to environmental changes (e.g., capture and transportation). Short-term or acute changes in physiological indices (e.g., heart rate, respiration, body temperatures, immune cells, and stress hormonal biomarkers) provide crucial information regarding the responses of animals to novel environments, and they could provide crucial determining factors for the long-term health and welfare of animals. This Special Issue includes experimental research papers that demonstrate the applications of physiological indices and welfare assessment methods (e.g., morphological and morphometric data, behavioural assessments, thermal profiles, and physiological markers) in any wildlife or production animal (e.g., rescued and rehabilitating animals, pets, competition animals, farm animals, and zoo animals), in response to environmental and management related factors. The goal is to provide examples of new research and techniques that can be used to monitor short- and long-term environmental adaptation of animals under human care.

Keywords

Research & information: general --- Biology, life sciences --- Zoology & animal sciences --- non-invasive --- bioindicator --- pollution --- stress --- welfare --- constructed wetland --- glucocorticoid --- urban river --- reindeer --- glucocorticoids --- validation --- rodents --- ecological immunology --- natural antibodies --- haptoglobin --- neutrophil to lymphocyte ratio --- immune strategy --- vector-borne pathogens --- parasitology --- zoonosis --- thermal imaging --- koalas --- body temperature --- heat/cold stress --- thermoregulation --- substrate --- Zoo --- protocol --- veterinary assessment --- Punjab urial --- body condition --- behavior --- Pakistan --- red deer --- hind --- reproduction --- progesterone --- cortisol --- hair --- Asian elephant --- saliva --- immunoglobulin A --- circadian rhythm --- Panthera tigris tigris --- Panthera tigris altaica --- siberian --- tigers --- bengal tigers --- captive --- biochemical parameter --- serum protein electrophoresis --- Cervus elaphus --- plasma --- feces --- hunting --- zoo --- faecal analysis --- glucocorticoid metabolites --- enzyme immunoassay --- roe deer --- blood parameters --- prognostic factors --- blood lactate concentration --- biomarkers --- captivity-induced stress --- fecal glucocorticoid metabolites --- physiological stress in rodents --- oxytocin --- boar --- ejaculation --- wildlife --- environmental stress --- urbanisation --- birds --- non-invasive --- bioindicator --- pollution --- stress --- welfare --- constructed wetland --- glucocorticoid --- urban river --- reindeer --- glucocorticoids --- validation --- rodents --- ecological immunology --- natural antibodies --- haptoglobin --- neutrophil to lymphocyte ratio --- immune strategy --- vector-borne pathogens --- parasitology --- zoonosis --- thermal imaging --- koalas --- body temperature --- heat/cold stress --- thermoregulation --- substrate --- Zoo --- protocol --- veterinary assessment --- Punjab urial --- body condition --- behavior --- Pakistan --- red deer --- hind --- reproduction --- progesterone --- cortisol --- hair --- Asian elephant --- saliva --- immunoglobulin A --- circadian rhythm --- Panthera tigris tigris --- Panthera tigris altaica --- siberian --- tigers --- bengal tigers --- captive --- biochemical parameter --- serum protein electrophoresis --- Cervus elaphus --- plasma --- feces --- hunting --- zoo --- faecal analysis --- glucocorticoid metabolites --- enzyme immunoassay --- roe deer --- blood parameters --- prognostic factors --- blood lactate concentration --- biomarkers --- captivity-induced stress --- fecal glucocorticoid metabolites --- physiological stress in rodents --- oxytocin --- boar --- ejaculation --- wildlife --- environmental stress --- urbanisation --- birds

The Elisa : enzyme-linked immunosorbent assay in veterinary research and diagnosis : proceedings of a meeting held at the University of Surrey, Guildford, UK, september 15-17 1981
Authors: ---
ISBN: 9024727693 Year: 1982 Publisher: Dordrecht : Martinus Nijhoff,


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Cow's Milk and Allergy
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ISBN: 3039280295 3039280287 Year: 2020 Publisher: MDPI - Multidisciplinary Digital Publishing Institute

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The purpose of this Special Issue “Cow’s Milk and Allergy” is to provide an overview of the association of cow’s milk with allergy. This topic has two quite different faces. On the one hand, we are all aware of the importance of cow’s milk allergy in early life. What is less known is that the consumption of raw, unprocessed milk is associated with a lower incidence of asthma and rhinitis. This Special Issue takes a closer look at all of these aspects of cow’s milk and allergy and focus on the following questions:


Book
Assessing the Environmental Adaptation of Wildlife and Production Animals : Applications of Physiological Indices and Welfare Assessment Tools
Author:
Year: 2021 Publisher: Basel, Switzerland MDPI - Multidisciplinary Digital Publishing Institute

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Abstract

Wild animals under human care as well as domesticated farm production animals are often exposed to environmental changes (e.g., capture and transportation). Short-term or acute changes in physiological indices (e.g., heart rate, respiration, body temperatures, immune cells, and stress hormonal biomarkers) provide crucial information regarding the responses of animals to novel environments, and they could provide crucial determining factors for the long-term health and welfare of animals. This Special Issue includes experimental research papers that demonstrate the applications of physiological indices and welfare assessment methods (e.g., morphological and morphometric data, behavioural assessments, thermal profiles, and physiological markers) in any wildlife or production animal (e.g., rescued and rehabilitating animals, pets, competition animals, farm animals, and zoo animals), in response to environmental and management related factors. The goal is to provide examples of new research and techniques that can be used to monitor short- and long-term environmental adaptation of animals under human care.


Book
Application of Analytical Chemistry to Foods and Food Technology
Authors: ---
Year: 2021 Publisher: Basel, Switzerland MDPI - Multidisciplinary Digital Publishing Institute

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Abstract

The application of analytical chemistry to the food sector allows the determination of the chemical composition of foods and the properties of their constituents, contributing to the definition of their nutritional and commodity value. Furthermore, it is possible to study the chemical modifications that food constituents undergo as a result of the treatments they undergo (food technology). Food analysis, therefore, allows us not only to determine the quality of a product or its nutritional value, but also to reveal adulterations and identify the presence of xenobiotic substances potentially harmful to human health. Furthermore, some foods, especially those of plant origin, contain numerous substances with beneficial effects on health. While these functional compounds can be obtained from a correct diet, they can also be extracted from food matrices for the formulation of nutraceutical products or added to foods by technological or biotechnological means for the production of functional foods. On the other hand, the enormous growth of the food industry over the last 50 years has broadened the field of application of analytical chemistry to encompass not only food but also food technology, which is fundamental for increasing the production of all types of food.

Keywords

Humanities --- Social interaction --- solid-liquid extraction --- green extraction --- RSLDE --- bioactive compounds --- Naviglio extractor --- Naviglio’s principle --- Hsian-tsao --- Platostoma palustre (Blume) --- headspace solid-phase microextraction (SPME) --- volatile components --- simultaneous distillation-extraction (SDE) --- amino acid profiling --- hydrophilic interaction chromatography (HILIC) --- tandem mass spectrometry --- Triticum species flours --- flour quality characteristics --- narrow-leaved oleaster fruits --- near-infrared hyperspectral imaging --- geographical origin --- convolutional neural network --- effective wavelengths --- food colorants (synthetic, natural) --- food matrices --- instrumental analysis --- sample preparation --- mango --- volatile compounds --- frequency detection (FD) --- order-specific magnitude estimation (OSME) --- odor activity value --- sensory analysis --- lead (II) --- ELISA --- monoclonal antibody (mAb) --- isothiocyanobenzyl-EDTA (ITCBE) --- chemiluminescent enzyme immunoassay (CLEIA) --- meadow saffron --- metabolomics --- UHPLC-QTOF-mass spectrometry --- extraction methods --- antioxidants --- Pressurized liquid extraction --- soxhlet --- solvent extraction --- green analytical chemistry --- Rosemary --- poultry eggs --- spectinomycin --- lincomycin --- ASE --- GC-EI/MS/MS --- acrylamide --- kobbah --- transglutaminase --- pectin --- chitosan-nanoparticles --- coatings --- mesoporous silica nanoparticles --- grass pea --- HPLC-RP --- Curcuma longa L. --- curcuminoid stability --- multi-step extraction --- ultrasound-assisted extraction --- extraction kinetic --- functional foods --- gas chromatography --- health effects --- liquid chromatography (HPLC) --- mass spectrometry --- nutraceuticals --- phytochemicals --- solid-liquid extraction techniques --- solid-liquid extraction --- green extraction --- RSLDE --- bioactive compounds --- Naviglio extractor --- Naviglio’s principle --- Hsian-tsao --- Platostoma palustre (Blume) --- headspace solid-phase microextraction (SPME) --- volatile components --- simultaneous distillation-extraction (SDE) --- amino acid profiling --- hydrophilic interaction chromatography (HILIC) --- tandem mass spectrometry --- Triticum species flours --- flour quality characteristics --- narrow-leaved oleaster fruits --- near-infrared hyperspectral imaging --- geographical origin --- convolutional neural network --- effective wavelengths --- food colorants (synthetic, natural) --- food matrices --- instrumental analysis --- sample preparation --- mango --- volatile compounds --- frequency detection (FD) --- order-specific magnitude estimation (OSME) --- odor activity value --- sensory analysis --- lead (II) --- ELISA --- monoclonal antibody (mAb) --- isothiocyanobenzyl-EDTA (ITCBE) --- chemiluminescent enzyme immunoassay (CLEIA) --- meadow saffron --- metabolomics --- UHPLC-QTOF-mass spectrometry --- extraction methods --- antioxidants --- Pressurized liquid extraction --- soxhlet --- solvent extraction --- green analytical chemistry --- Rosemary --- poultry eggs --- spectinomycin --- lincomycin --- ASE --- GC-EI/MS/MS --- acrylamide --- kobbah --- transglutaminase --- pectin --- chitosan-nanoparticles --- coatings --- mesoporous silica nanoparticles --- grass pea --- HPLC-RP --- Curcuma longa L. --- curcuminoid stability --- multi-step extraction --- ultrasound-assisted extraction --- extraction kinetic --- functional foods --- gas chromatography --- health effects --- liquid chromatography (HPLC) --- mass spectrometry --- nutraceuticals --- phytochemicals --- solid-liquid extraction techniques


Book
Application of Analytical Chemistry to Foods and Food Technology
Authors: ---
Year: 2021 Publisher: Basel, Switzerland MDPI - Multidisciplinary Digital Publishing Institute

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Abstract

The application of analytical chemistry to the food sector allows the determination of the chemical composition of foods and the properties of their constituents, contributing to the definition of their nutritional and commodity value. Furthermore, it is possible to study the chemical modifications that food constituents undergo as a result of the treatments they undergo (food technology). Food analysis, therefore, allows us not only to determine the quality of a product or its nutritional value, but also to reveal adulterations and identify the presence of xenobiotic substances potentially harmful to human health. Furthermore, some foods, especially those of plant origin, contain numerous substances with beneficial effects on health. While these functional compounds can be obtained from a correct diet, they can also be extracted from food matrices for the formulation of nutraceutical products or added to foods by technological or biotechnological means for the production of functional foods. On the other hand, the enormous growth of the food industry over the last 50 years has broadened the field of application of analytical chemistry to encompass not only food but also food technology, which is fundamental for increasing the production of all types of food.

Keywords

Humanities --- Social interaction --- solid-liquid extraction --- green extraction --- RSLDE --- bioactive compounds --- Naviglio extractor --- Naviglio’s principle --- Hsian-tsao --- Platostoma palustre (Blume) --- headspace solid-phase microextraction (SPME) --- volatile components --- simultaneous distillation-extraction (SDE) --- amino acid profiling --- hydrophilic interaction chromatography (HILIC) --- tandem mass spectrometry --- Triticum species flours --- flour quality characteristics --- narrow-leaved oleaster fruits --- near-infrared hyperspectral imaging --- geographical origin --- convolutional neural network --- effective wavelengths --- food colorants (synthetic, natural) --- food matrices --- instrumental analysis --- sample preparation --- mango --- volatile compounds --- frequency detection (FD) --- order-specific magnitude estimation (OSME) --- odor activity value --- sensory analysis --- lead (II) --- ELISA --- monoclonal antibody (mAb) --- isothiocyanobenzyl-EDTA (ITCBE) --- chemiluminescent enzyme immunoassay (CLEIA) --- meadow saffron --- metabolomics --- UHPLC-QTOF-mass spectrometry --- extraction methods --- antioxidants --- Pressurized liquid extraction --- soxhlet --- solvent extraction --- green analytical chemistry --- Rosemary --- poultry eggs --- spectinomycin --- lincomycin --- ASE --- GC-EI/MS/MS --- acrylamide --- kobbah --- transglutaminase --- pectin --- chitosan-nanoparticles --- coatings --- mesoporous silica nanoparticles --- grass pea --- HPLC-RP --- Curcuma longa L. --- curcuminoid stability --- multi-step extraction --- ultrasound-assisted extraction --- extraction kinetic --- functional foods --- gas chromatography --- health effects --- liquid chromatography (HPLC) --- mass spectrometry --- nutraceuticals --- phytochemicals --- solid-liquid extraction techniques


Book
Application of Analytical Chemistry to Foods and Food Technology
Authors: ---
Year: 2021 Publisher: Basel, Switzerland MDPI - Multidisciplinary Digital Publishing Institute

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Abstract

The application of analytical chemistry to the food sector allows the determination of the chemical composition of foods and the properties of their constituents, contributing to the definition of their nutritional and commodity value. Furthermore, it is possible to study the chemical modifications that food constituents undergo as a result of the treatments they undergo (food technology). Food analysis, therefore, allows us not only to determine the quality of a product or its nutritional value, but also to reveal adulterations and identify the presence of xenobiotic substances potentially harmful to human health. Furthermore, some foods, especially those of plant origin, contain numerous substances with beneficial effects on health. While these functional compounds can be obtained from a correct diet, they can also be extracted from food matrices for the formulation of nutraceutical products or added to foods by technological or biotechnological means for the production of functional foods. On the other hand, the enormous growth of the food industry over the last 50 years has broadened the field of application of analytical chemistry to encompass not only food but also food technology, which is fundamental for increasing the production of all types of food.

Keywords

solid-liquid extraction --- green extraction --- RSLDE --- bioactive compounds --- Naviglio extractor --- Naviglio’s principle --- Hsian-tsao --- Platostoma palustre (Blume) --- headspace solid-phase microextraction (SPME) --- volatile components --- simultaneous distillation-extraction (SDE) --- amino acid profiling --- hydrophilic interaction chromatography (HILIC) --- tandem mass spectrometry --- Triticum species flours --- flour quality characteristics --- narrow-leaved oleaster fruits --- near-infrared hyperspectral imaging --- geographical origin --- convolutional neural network --- effective wavelengths --- food colorants (synthetic, natural) --- food matrices --- instrumental analysis --- sample preparation --- mango --- volatile compounds --- frequency detection (FD) --- order-specific magnitude estimation (OSME) --- odor activity value --- sensory analysis --- lead (II) --- ELISA --- monoclonal antibody (mAb) --- isothiocyanobenzyl-EDTA (ITCBE) --- chemiluminescent enzyme immunoassay (CLEIA) --- meadow saffron --- metabolomics --- UHPLC-QTOF-mass spectrometry --- extraction methods --- antioxidants --- Pressurized liquid extraction --- soxhlet --- solvent extraction --- green analytical chemistry --- Rosemary --- poultry eggs --- spectinomycin --- lincomycin --- ASE --- GC-EI/MS/MS --- acrylamide --- kobbah --- transglutaminase --- pectin --- chitosan-nanoparticles --- coatings --- mesoporous silica nanoparticles --- grass pea --- HPLC-RP --- Curcuma longa L. --- curcuminoid stability --- multi-step extraction --- ultrasound-assisted extraction --- extraction kinetic --- functional foods --- gas chromatography --- health effects --- liquid chromatography (HPLC) --- mass spectrometry --- nutraceuticals --- phytochemicals --- solid-liquid extraction techniques

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