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This book explains genetic engineering in a manner that is easily understandable and tailored to a general audience. It provides background on the history and current state of genetic engineering, and encourages open dialogue on the risks and future of genetic engineering.

Genetics --- Biotechnology --- genetica --- biotechnologie --- Genetic engineering. --- Enginyeria genètica

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This handbook provides comprehensive reference information on the efficient production of secondary metabolites from plants by transgenesis and other genetic manipulation strategies. It reviews and summarizes selected important case studies in genetic methods applied to enhance the production of a given metabolite or a group of related or derived compounds. Readers will find reference information on a multitude of techniques and methods, including traditional breeding and screening; over-expression of genes encoding key enzymes; functional genomics approaches; metabolic engineering of the relevant biosynthetic pathways; indirect genetic approaches to improve metabolite production, including Agrobacterium mediated transformations. Furthermore, combinatorial biochemistry approaches to engineer secondary metabolic pathways are summarized, which can offer access to new structures or to the design of novel compounds. Since many commercially valuable substances are still extracted from plants, being largely inaccessible to efficient modern laboratory synthesis methods, this book provides a valuable resource of information for biotechnological approaches that can help to find alternative and more efficient methods for the production of natural secondary metabolites. Thus adjusted production methods, with the help of tailored plant systems, can potentially help to release the stress on plants, which are curre ntly suffering from extensive human harvesting, and to conserve global biodiversity. Readers will find comprehensive reference information on plant genetic manipulation toward more efficient synthesis, accumulation and production of target secondary metabolites. The handbook will appeal to researchers and professionals, but also graduate students and scholars working in the fields of biotechnology, genetic engineering, medicinal plant research, pharmacy, and phytochemistry.

Genetic engineering. --- Plant genetics. --- Plant biochemistry. --- Metabolism. --- Bioorganic chemistry. --- Pharmacy. --- Genetic Engineering. --- Plant Genetics and Genomics. --- Plant Biochemistry. --- Metabolomics. --- Bioorganic Chemistry. --- Enginyeria genètica --- Enginyeria genètica vegetal --- Botanical chemistry.

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Crops --- Genetic engineering. --- Plant genetic engineering --- Plantes transgèniques --- Conreu --- Enginyeria genètica vegetal

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This detailed volume collects commonly used and cutting-edge methods to analyze alternative splicing, a key step in gene regulation. After an introduction of the alternative splicing mechanism and its targeting for therapeutic strategies, the book continues with techniques for analyzing alternative splicing profiles in complex biological systems, visualizing and localizing alternative spliced transcripts with cellular and sub-cellular resolution, probing regulators of alternative splicing, as well as assessing the functional consequences of alternative splicing. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Alternative Splicing: Methods and Protocols serves as an ideal guide for both RNA aficionados that want to implement novel approaches in their labs and novices undertaking alternative splicing projects.

Genetics. --- Genomics. --- Genetics and Genomics. --- Genome research --- Genomes --- Molecular genetics --- Biology --- Embryology --- Mendel's law --- Adaptation (Biology) --- Breeding --- Chromosomes --- Heredity --- Mutation (Biology) --- Variation (Biology) --- Research --- Enginyeria genètica --- Intervenció genètica --- Manipulació genètica --- Recombinació genètica --- ADN recombinant --- Clonatge --- Clonatge molecular --- Enginyeria de proteïnes --- Enginyeria genètica animal --- Enginyeria genètica vegetal --- Teràpia genètica --- Fecundació in vitro --- Organismes transgènics

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This volume explores homologous recombination’s (HR) essential role in meiotic and somatic cells. It discusses the analysis of different steps of the HR process from the genetic, molecular biology, and cell biology perspectives. Using a variety of model systems, chapters in this book cover topics such as the genome-wide mapping of DNA double-strand breaks (DSB); analysis of DNA-end resection and recombination intermediates by gel electrophoresis and southern blotting; cell-based monitoring of HR activity in meiotic and mitotic cells; in vitro analysis of branch migration, DNA strand exchange and Holliday junction resolution; or super resolution imaging of HR repair at collapsed replication forks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Homologous Recombination: Methods and Protocols is a valuable resource that uses both classical and more recent approaches to answer questions on the HR mechanism. It is a useful tool for scientists working on the field of genome integrity, as well as those working in cancer biology and DNA repair. .

Recombinació genètica --- Cromosomes --- Enginyeria genètica --- ADN recombinant --- Medical genetics. --- Medical Genetics. --- Clinical genetics --- Diseases --- Heredity of disease --- Human genetics --- Medical sciences --- Pathology --- Genetic disorders --- Genetic aspects