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Electrophoresis, Polyacrylamide Gel --- Immunoelectrophoresis --- Proteins --- methods
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A low molecular weight β2-globulin which goes not correspond to any known protein has been isolated from urine of a cadmium-exposed worker with proteinuria. The isolation procedure included chromatography on Sephadex G-75 and DEAE-cellulose, followed by preparative agarose gel electrophoresis.
The purified protein was homogeneous in ultracentrifugation and in electrophoresis in agarose gel. Electrophoresis in polyacrylamide gel containing sodium dodecyl sulphate confirmed the homogeneity of the preparation and suggested a molecular weight of about 21.000. No carbohydrate was detected by the anthrone reaction. Absorbance was maximal at 279 nm. This protein is apparently devoid of cysteine, methionine and histidne. Trace amounts of the protein have been found in normal human urine
Cadmium --- Proteinuria --- Electrophoresis, Polyacrylamide Gel --- Sodium Dodecyl Sulfate --- Molecular Weight
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EPIDEMIOLOGY, MOLECULAR --- SEQUENCE ANALYSIS, DNA --- DNA FINGER PRINTING --- DNA PROBES --- DNA PROBES, HLA --- ELECTROPHORESIS, POLYACRYLAMIDE GEL --- DIAGNOSTIC USE --- METHODS --- EPIDEMIOLOGY, MOLECULAR --- SEQUENCE ANALYSIS, DNA --- DNA FINGER PRINTING --- DNA PROBES --- DNA PROBES, HLA --- ELECTROPHORESIS, POLYACRYLAMIDE GEL --- DIAGNOSTIC USE --- METHODS
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Analytical biochemistry --- Clinical chemistry --- Electrophoresis, Polyacrylamide gel --- Isoelectric focusing --- Congresses --- Polyacrylamide gel electrophoresis --- Electrophoresis, Polyacrylamide Gel. --- Isoelectric Focusing. --- -Polyacrylamide gel electrophoresis --- -Electrophoresis, Polyacrylamide gel --- PAGE (Biochemistry) --- Polyacrylamide disc electrophoresis --- Gel electrophoresis --- Zone electrophoresis --- Electrofocusing --- Focusing, Isoelectric --- Chemistry, Analytic --- Chemistry, Organic --- Electrophoresis --- Polyacrylamide Gel Electrophoresis --- SDS-PAGE --- Sodium Dodecyl Sulfate-PAGE --- Gel Electrophoresis, Polyacrylamide --- SDS PAGE --- Sodium Dodecyl Sulfate PAGE --- Sodium Dodecyl Sulfate-PAGEs --- Qualitative --- Eiwitten --- Elektroforese --- Congresses. --- Eiwitten. --- Elektroforese. --- -Congresses --- Electrophoresis, Polyacrylamide Gel --- Isoelectric Focusing --- Analytical chemistry --- Polyacrylamide gel electrophoresis - Congresses --- Isoelectric focusing - Congresses
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Polyacrylamide gel electrophoresis --- Proteins --- Analysis --- Polyacrylamide gel electrophoresis. --- Analysis. --- Electrophoresis, Polyacrylamide gel --- PAGE (Biochemistry) --- Polyacrylamide disc electrophoresis --- Gel electrophoresis --- Zone electrophoresis --- Proteins - Analysis
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Gel electrophoresis --- Polyacrylamide gel electrophoresis --- Proteins --- Electrophorèse en gel --- Electrophorèse sur gel de polyacrylamide --- Protéines --- Analysis --- Analyse --- Electrophoresis, Polyacrylamide gel --- Immunoelectrophoresis --- Electrophoresis, Polyacrylamide Gel --- Electrophorèse en gel d'acrylamide --- Immunoélectrophorèse --- isolation & purification --- Electrophoresis, Polyacrylamide Gel. --- Polyacrylamide Gel Electrophoresis --- SDS-PAGE --- Sodium Dodecyl Sulfate-PAGE --- Gel Electrophoresis, Polyacrylamide --- SDS PAGE --- Sodium Dodecyl Sulfate PAGE --- Sodium Dodecyl Sulfate-PAGEs --- Polyacrylamide gel electrophoresis. --- Electrophorèse en gel d'acrylamide --- Immunoélectrophorèse --- Proteins. --- 577.112 --- 577.112 Proteins --- Gene Products, Protein --- Gene Proteins --- Protein Gene Products --- Proteins, Gene --- Molecular Mechanisms of Pharmacological Action --- 57.088 --- 57.088 Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- Analytical biochemistry --- Eiwitten. --- Elektroforese. --- methods --- laboratory manuals. --- Protein --- Electrophoresis, Polyacrylamide Gel - laboratory manuals --- Proteins - laboratory manuals
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DNA. --- Electrophoresis, Polyacrylamide Gel. --- Nucleoproteins. --- RNA. --- Gene Products, RNA --- Non-Polyadenylated RNA --- RNA, Non-Polyadenylated --- Ribonucleic Acid --- Acid, Ribonucleic --- Non Polyadenylated RNA --- RNA Gene Products --- RNA, Non Polyadenylated --- Biopolymers --- Nucleoprotein --- Polyacrylamide Gel Electrophoresis --- SDS-PAGE --- Sodium Dodecyl Sulfate-PAGE --- Gel Electrophoresis, Polyacrylamide --- SDS PAGE --- Sodium Dodecyl Sulfate PAGE --- Sodium Dodecyl Sulfate-PAGEs --- ds-DNA --- DNA, Double-Stranded --- Deoxyribonucleic Acid --- DNA, Double Stranded --- Double-Stranded DNA --- ds DNA --- Analytical biochemistry --- Proteins --- Gel electrophoresis. --- Polyacrylamide gel electrophoresis. --- Analysis. --- Elektroforese. --- Nucleinezuren. --- DNA --- Electrophoresis, Polyacrylamide Gel --- Nucleoproteins --- RNA --- Amino acid sequence --- Rna --- GEL ELECTROPHORESIS --- NUCLEIC ACIDS --- ANALYSIS
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Now in a new second edition, this popular book includes a wealth of practical information on the much-used techniques of gel electrophoresis. Authoritative and completely current, the volume provides readers with an up-to-date, "how-to" guide to this growing field.
Nucleic acids --- Gel electrophoresis --- Acides nucléiques --- Electrophorèse en gel --- Analysis --- Analyse --- DNA. --- Electrophoresis, Polyacrylamide Gel. --- Nucleoproteins. --- RNA. --- -577.113 --- 57.088.3 --- 543.545 --- Elektroforese --- Polynucleotides --- Biomolecules --- Electrophoresis --- Gene Products, RNA --- Non-Polyadenylated RNA --- RNA, Non-Polyadenylated --- Ribonucleic Acid --- Acid, Ribonucleic --- Non Polyadenylated RNA --- RNA Gene Products --- RNA, Non Polyadenylated --- Biopolymers --- Nucleoprotein --- Polyacrylamide Gel Electrophoresis --- SDS-PAGE --- Sodium Dodecyl Sulfate-PAGE --- Gel Electrophoresis, Polyacrylamide --- SDS PAGE --- Sodium Dodecyl Sulfate PAGE --- Sodium Dodecyl Sulfate-PAGEs --- ds-DNA --- DNA, Double-Stranded --- Deoxyribonucleic Acid --- DNA, Double Stranded --- Double-Stranded DNA --- ds DNA --- Methods and techniques for separation,fractionation and purification. --- Electrophoretic analysis. Zone electrophoresis. Ionophoresis. Electrochromatography --- Gel electrophoresis. --- Basic Sciences. Chemistry --- Analysis. --- Analytical Chemistry --- Analytical Chemistry. --- 543.545 Electrophoretic analysis. Zone electrophoresis. Ionophoresis. Electrochromatography --- 57.088.3 Methods and techniques for separation,fractionation and purification. --- 577.113 Nucleic acids --- Acides nucléiques --- Electrophorèse en gel --- DNA --- Electrophoresis, Polyacrylamide Gel --- Nucleoproteins --- RNA --- 577.113 --- Methods and techniques for separation,fractionation and purification
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Here is a newly updated and expanded edition of a useful and popular guide. In addition to providing new information on topics covered in the first edition, this volume covers a number of major techniques which must be mastered by molecular biologists and biochemists. All the main aspects of polyacrylamide gel electrophoresis are described, while valuable appendix materials bring the reader useful listings of suppliers' addresses, detection methods, and more.
General biochemistry --- gelelektroforese --- biochemie --- polyacrylamidegel --- proteïnen --- isoelectric focusing --- IEP (immunoelektrophorese) --- Gel electrophoresis --- Polyacrylamide gel electrophoresis --- Proteins --- Electrophorèse en gel --- Electrophorèse sur gel de polyacrylamide --- Protéines --- Analysis --- Analyse --- Gel electrophoresis. --- Polyacrylamide gel electrophoresis. --- Analysis. --- Électrophorèse --- Electrophoresis --- Protéine --- proteins --- Technique analytique --- Analytical methods --- 577.112 --- 57.088 --- 543.545 --- Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- Electrophoretic analysis. Zone electrophoresis. Ionophoresis. Electrochromatography --- Elektroforese: proteïnen --- Basic Sciences. Chemistry --- Biochemistry --- Biochemistry. --- 543.545 Electrophoretic analysis. Zone electrophoresis. Ionophoresis. Electrochromatography --- 57.088 Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- 577.112 Proteins --- Electrophorèse en gel --- Electrophorèse sur gel de polyacrylamide --- Protéines --- Electrophoresis, Polyacrylamide gel --- PAGE (Biochemistry) --- Polyacrylamide disc electrophoresis --- Zone electrophoresis --- Proteins - Analysis. --- PAGE --- LABORATORY MANUALS --- PROTEINS --- GEL ELECTROPHORESIS --- METHODS
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