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Over the past several decades, advanced molecular techniques in diagnostic microbiology quietly have been revolutionizing the practice of clinical microbiology in the hospital setting. Indeed, molecular diagnostic testing in general and nucleic-acid-based amplification methods in particular have been heralded as diagnostic tools for the new millennium. There is no question that the development of rapid molecular techniques for nucleic acid amplification/characterization combined with automation and user-friendly software has greatly broadened the diagnostic capabilities of the clinical microbiology laboratory. These technical advances in molecular microbiology over the first decade of the 21st Century have profoundly influenced the physical structure of clinical microbiology laboratories as well as their staffing patterns, workflow, and turnaround time. These molecular microbiology advances have also resulted in the need for a revised and updated second edition of Advanced Techniques in Diagnostic Microbiology. This second edition provides an updated and comprehensive description of the ongoing evolution of molecular methods for the diagnosis of infectious diseases. In addition, many new chapters have been added, including a chapter on the clinical interpretation and relevance of advanced technique results. The second edition, like the first edition, includes both a “techniques” section describing the latest molecular techniques and an “applications” section describing how these advanced molecular techniques are being used in the clinical setting. Finally, the second edition, like the first edition, utilizes a diverse team of authors who have compiled chapters that provide the reader with comprehensive and useable information on advanced molecular microbiology techniques.

Diagnostic microbiology -- Technique. --- Diagnostic microbiology --- Clinical Laboratory Techniques --- Diagnosis --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Diagnostic Techniques and Procedures --- Microbiological Techniques --- Biology --- Health & Biological Sciences --- Microbiology & Immunology --- Technique --- Technique. --- Clinical microbiology --- Microbiology, Clinical --- Microbiology, Diagnostic --- Medicine. --- Medical microbiology. --- Biomedicine. --- Medical Microbiology. --- Diagnosis, Laboratory --- Medical microbiology --- Communicable diseases --- Pathogenic microorganisms --- Serology --- Microbiology. --- Microbial biology --- Microorganisms

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Which disease should you consider when a young adult has localized bone pain that is dramatically relieved by aspirin? What is sometimes the only physical manifestation of bulimia nervosa? These so-called "old fashioned" historical clues and physical signs are not only part of our medical heritage; their deployment can often spell the difference between prompt clinical diagnosis versus a costly investigative odyssey. Often overlooked with today's emphasis on laboratory testing, a diagnostic pearl such as knowing that a diabetic patient with peripheral neuropathy may actually have metformin-induced vitamin B12 deficiency can be a shortcut to disease recognition. Diagnostic Principles and Applications presents selected literature-based facts and diagnostic insights to help clinicians provide better-informed clinical assessments, avoid medical misadventures, and prepare for their board examinations. The chapters cover: Classic diagnostic pearls Red flag symptoms and signs of serious illness Bellwether disease indications allowing an occasional early, preemptive diagnosis Counterintuitive clinical manifestations of disease Curious clues to specific diagnoses Robert B. Taylor, MD is the author and editor of more than two dozen medical books, including three on the topic of clinical diagnosis, and several hundred published articles. He is a veteran of both rural private practice and chairmanship of a medical school clinical department.

Communicable diseases -- Diagnosis -- Laboratory manuals. --- Diagnostic microbiology -- Technique. --- DNA probes -- Diagnostic use. --- Nucleic acid hybridization. --- Diagnosis --- Pathological Conditions, Signs and Symptoms --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Diseases --- Signs and Symptoms --- Medicine --- Health & Biological Sciences --- Medical Professional Practice --- Examinations --- Diagnosis. --- Examinations, Medical (Diagnosis) --- Medical diagnosis --- Medical examinations (Diagnosis) --- Medical tests (Diagnosis) --- Testing --- Medicine. --- General practice (Medicine). --- Primary care (Medicine). --- Medicine & Public Health. --- General Practice / Family Medicine. --- Primary Care Medicine. --- Medicine/Public Health, general. --- Clinical medicine --- Prognosis --- Symptoms --- Family medicine. --- Emergency medicine. --- Clinical sciences --- Medical profession --- Human biology --- Life sciences --- Medical sciences --- Pathology --- Physicians --- Medicine, Emergency --- Critical care medicine --- Disaster medicine --- Medical emergencies --- Family practice (Medicine) --- General practice (Medicine) --- Physicians (General practice) --- Health Workforce --- Primary medical care --- Medical care