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Amino Acid Analysis (AAA) is an integral part of analytical biochemistry. In a relatively short time, the variety of AAA methods has evolved dramatically with more methods shifting to the use of mass spectrometry (MS) as a detection method. Another new aspect is miniaturization. However, most importantly, AAA in this day and age should be viewed in the context of Metabolomics as a part of Systems Biology. Amino Acid Analysis: Methods and Protocols, Second Edition presents a broad spectrum of all available methods allowing readers to choose the method that best suits their particular laboratory set-up and analytical needs. In this volume, readers can find chapters describing general, as well as specific approaches to the sample preparation. A number of chapters describe specific applications of AAA in clinical chemistry, as well as in food analysis, microbiology, marine biology, drug metabolism, even archaeology. Separate chapters are devoted to the application of AAA for protein quantitation and chiral AAA. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Amino Acid Analysis: Methods and Protocols, Second Edition provides crucial techniques that can be applied across multiple disciplines by anyone involved in biomedical research or life sciences.

Biochemistry. --- Mass spectrometry. --- Biochemistry, general. --- Mass Spectrometry. --- Mass spectra --- Mass spectrograph --- Mass spectroscopy --- Mass spectrum analysis --- Mass (Physics) --- Nuclear spectroscopy --- Spectrum analysis --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition --- Aminoàcids --- Bioquímica analítica --- Espectrometria de masses --- Manuals de laboratori. --- Tècniques de laboratori --- Espectrògraf de masses --- Espectroscòpia --- Espectroscòpia nuclear --- Desorció-ionització amb làser assistit per matriu --- Espectrometria de masses amb ionització per electroesprai --- Espectrometria de masses de plasma acoblat inductivament --- Espectrometria de masses de temps de vol --- Anàlisi bioquímica --- Bioquímica --- Química analítica --- Àcids aminats --- Àcid glutàmic --- Aminoàcids essencials --- Aminoàcids sulfurosos --- Cisteïna --- Glutamina --- Protamines

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This book focuses on ion mobility-mass spectrometry (IM-MS) and informatics approaches to improve traditional analysis of molecules by providing fundamentals and protocols for exploiting the potential of state-of-the-art IM-MS technology for the most common analytical applications. The chapters have been organized into four parts, each dealing with a particular set of IM-MS applications: 1) metabolomics and lipidomics; 2) proteomics and glycomics; 3) imaging and ambient ionization IM-MS; and 4) bioinformatic solutions for analyzing IM-MS data and deriving CCS values. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Ion Mobility-Mass Spectrometry: Methods and Protocols serves as an ideal resource for scientists delving into the technique's unprecedented analytical advantages, enabling novel qualitative and quantitative applications for the analysis of complex biological samples.

Espectroscòpia de mobilitat d'ions --- Manuals de laboratori --- Protocols clínics --- Plans d'actuació mèdica --- Protocols en medicina --- Protocols de diagnòstic clínic --- Protocols mèdics --- Protocols terapèutics --- Medicina clínica --- Històries clíniques --- Protocols d'infermeria --- Tècniques de laboratori --- Espectrometria de mobilitat d'ions --- Espectroscòpia --- Biochemistry. --- Mass spectroscopy. --- Mass Spectrometry. --- Mass spectra --- Mass spectrograph --- Mass spectroscopy --- Mass spectrum analysis --- Mass (Physics) --- Nuclear spectroscopy --- Spectrum analysis --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition --- Ions --- Espectrometria de masses --- Espectrògraf de masses --- Espectroscòpia nuclear --- Desorció-ionització amb làser assistit per matriu --- Espectrometria de masses amb ionització per electroesprai --- Espectrometria de masses de plasma acoblat inductivament --- Espectrometria de masses de temps de vol --- Propietats de la matèria --- Solucions (Química) --- Anions --- Bombardeig iònic --- Cations --- Descàrregues elèctriques a través de gasos --- Emissió termoiònica --- Ions metàl·lics --- Cristalls iònics --- Electròlisi --- Electrons --- Solucions iòniques