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Species Diagnostics Protocols: PCR and Other Nucleic Acid Methods is premised on the rapid development in recent years of the use of nucleic acid-based technology for the identification of various organisms. The majority of applications have been in such medically related fields as the diagnosis of disease or the identification of dis­ ease vectors, but this emphasis is changing as the potential and range of the techniques becomes more generally recognized. This has been especially true since the advent of polymerase chain reaction (PCR) technology. The use of nucleic acids for diagnostic purposes offers numerous advantages over conventional methods of identification. It must be stressed, however, that conventional methods are still extremely valuable and will always remain at the forefront of many diagnostic procedures. The ability of the skilled taxonomist begins to fail when there is a requirement to identify rapidly organisms that are small, immature, or present in large numbers. In such cases the time scale necessary to obtain an identification often precludes practical usage. It is in this context that molecular methods come into their own. There are several advantages that DNA can offer diagnostics other than the obvious and important fact that its base sequence is the fundamental source of biological variation. It offers a great amount of information that is not accessible to other methods of identifica­ tion because the vast majority of DNA is not expressed.

DNA probes. --- Microorganisms --- Polymerase chain reaction. --- Identification. --- Deoxyribonucleic acid probes --- Gene probes --- Genomic probes --- Probes, DNA --- Deoxyribonucleotides --- Nucleic acid probes --- Chain reaction, Polymerase --- PCR (Biochemistry) --- Polymerization --- DNA polymerases --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists

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DNA fingerprinting --- Rape --- Forensic genetics --- Evidence, Expert --- Forensic biology --- Genetics --- Medical jurisprudence --- DNA probes. --- DNA data banks. --- Criminals --- Parole --- Supervision of --- Crime and criminals --- Delinquents --- Offenders --- Persons --- Crime --- Criminal justice, Administration of --- Criminology --- DNA bank databases --- DNA banking databases --- DNA databanks --- DNA databases --- Databases --- Deoxyribonucleic acid probes --- Gene probes --- Genomic probes --- Probes, DNA --- Deoxyribonucleotides --- Nucleic acid probes

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Some of the more interesting elements in the chemistry of life are less commonly occuring ones such as nickel and molybdenum. This volume elucidates the chemistry of these elements in important enzymes and also explores the chemistry of elements that do not normally occur in biological molecules, but are useful in probing their structure and function. Topics include: Acquisition and transport of Ni. Mechanistic action of Ni in a wide variety of enzymes. Multielectron redox systems involving pterins in proteins. Chemistry of the pterin and flavin complexes of Mo, Fe, Cu and Ru ions. Replacement of iron in transferrin by a number of other metal ions. Use of polypyridyl complexes of ruthenium and other transition metals as probes of nucleic acid structure through photochemical reactions.

Acide désoxyribonucléique--Sondes --- DNA probes --- Deoxyribonucleic acid probes --- Desoxyribonucleïnezuur--Sondes --- Ions métalliques --- Metaalionen --- Metal ions --- Probes [ADN ] --- Sondes [ADN ] --- Bioinorganic chemistry. --- Enzymes. --- Molybdenum Enzymes. --- Proteins. --- Chimie bioinorganique. --- Enzymes à molybdène. --- Enzymes à nickel. --- Enzymes à molybdène. --- Enzymes à nickel. --- Métalloprotéines. --- Bioinorganic chemistry --- Proteins --- Molybdenum Enzymes --- Enzymes --- Nucleic Acid Probes --- Metalloproteins --- Nucleic Acids --- Molecular Probes --- Laboratory Chemicals --- Amino Acids, Peptides, and Proteins --- Nucleic Acids, Nucleotides, and Nucleosides --- Chemicals and Drugs --- Specialty Uses of Chemicals --- Chemical Actions and Uses --- Physical & Theoretical Chemistry --- Chemistry --- Physical Sciences & Mathematics --- Biotechnology --- Biochemistry. --- Inorganic chemistry. --- Molecular biology. --- Cell biology. --- Biophysics. --- Biological physics. --- Biochemistry, general. --- Inorganic Chemistry. --- Molecular Medicine. --- Cell Biology. --- Biological and Medical Physics, Biophysics. --- Biological physics --- Biology --- Medical sciences --- Physics --- Cell biology --- Cellular biology --- Cells --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biophysics --- Biomolecules --- Systems biology --- Inorganic chemistry --- Inorganic compounds --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Composition