Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

The development of molecular cloning technology in the early 1970s created a revolution in the biological and biomedical sciences that extends to this day. The contributions in this book provide the reader with a perspective on how pervasive the applications of molecular cloning have become. The contributions are organized in sections based on application, and range from cancer biology and immunology to plant and evolutionary biology. The chapters also cover a wide range of technical approaches, such as positional cloning and cutting edge tools for recombinant protein expression. This book should appeal to many researchers, who should find its information useful for advancing their fields.

Molecular cloning. --- Cloning, Molecular --- DNA cloning --- Gene cloning --- Cloning --- Genetic engineering --- Molecular genetics --- Clone cells --- Medical genetics

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Now fully updated to reflect recent advances, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.

Molecular cloning --- 575.5 --- Genetische manipulatie --- Klonen --- Cloning, Molecular --- DNA cloning --- Gene cloning --- Cloning --- Genetic engineering --- Molecular genetics --- Clone cells --- Molecular cloning.

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Molecular biology --- Analytical biochemistry --- Molecular cloning --- Polymerase chain reaction --- Clonage moléculaire --- Réaction en chaîne de la polymérase --- Laboratory manuals --- Manuels de laboratoire --- Chain reaction, Polymerase --- PCR (Biochemistry) --- Polymerization --- DNA polymerases --- Cloning, Molecular --- DNA cloning --- Gene cloning --- Cloning --- Genetic engineering --- Molecular genetics --- Clone cells --- Clonage moléculaire --- Réaction en chaîne de la polymérase

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Clear and concise, this easy-to-use text offers an introductory course on the language of gene cloning, covering microbial, plant, and animal systems. The essential concepts in biology relevant to the understanding of gene cloning are presented in a well-organized and accessible manner. This updated version of the first edition is an invaluable book for nonscientists as well as scientists with little background knowledge in gene cloning, providing a wealth of information for anyone wishing to gain proficiency in reading and speaking the language of gene cloning.

Molecular cloning. --- Cloning, Molecular --- DNA cloning --- Gene cloning --- Cloning --- Genetic engineering --- Molecular genetics --- Clone cells --- Biochemistry. --- Human genetics. --- Food science. --- Plant physiology. --- Zoology. --- Biochemistry, general. --- Human Genetics. --- Food Science. --- Plant Physiology. --- Biology --- Natural history --- Animals --- Botany --- Plants --- Physiology --- Science --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Composition --- Food—Biotechnology. --- Molecular cloning

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Molecular biology --- molecular cloning --- Cloning, Molecular. --- Clone cells --- Molecular Cloning --- Cosmids --- Cloning, Organism --- Eukaryotic cells. --- Eucaryotic cells --- Biological techniques --- Molecular cloning. --- Basic Sciences. Molecular Biology --- Molecular Biology (General). --- Eukaryotic cells --- Molecular cloning --- Cloning, Molecular --- DNA cloning --- Gene cloning --- Cloning --- Genetic engineering --- Molecular genetics --- Cells --- Protista --- #abib:almm --- DNA --- Amino Acid Sequence --- Eukaryotic Cells --- Methods