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Plant Endosomes: Methods and Protocols explores a collection of protocols and techniques to analyze in vivo trafficking of endocytic/endosomal cargo, including lipids, fluids, proteins, and ligands, ultrastructural features of endosomes by high-pressure freezing/freeze-substitution and electron tomography, as well as protein-protein interactions in the endosomal and endomembrane system. The volume continues with coverage of the sorting defects in the transport of vacuolar storage proteins, function conservation of plant endosomal proteins, endosomal trafficking during plant responses to pathogens, protein composition of endosomes and endocytic vesicles, ubiquitination of endosomal cargo proteins, and the identification of novel endosomal components by chemical genomics and proteomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Detailed and practical, Plant Endosomes: Methods and Protocols gathers contributions from many leading and emerging plant membrane trafficking researchers in order to promote and facilitate novel studies and ideas in this vital field.

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Nervous System Diseases --- Mitochondrial Diseases --- Muscular Diseases --- Subcellular Fractions --- Organelles --- Metabolic Diseases --- Diseases --- Cytoplasmic Structures --- Neuromuscular Diseases --- Musculoskeletal Diseases --- Cellular Structures --- Cells --- Cytoplasm --- Nutritional and Metabolic Diseases --- Anatomy --- Intracellular Space --- Mitochondria --- Mitochondrial Myopathies --- Neurodegenerative Diseases --- Degenerative Diseases, Central Nervous System --- Degenerative Diseases, Neurologic --- Degenerative Diseases, Spinal Cord --- Degenerative Neurologic Diseases --- Degenerative Neurologic Disorders --- Nervous System Degenerative Diseases --- Neurodegenerative Disorders --- Neurologic Degenerative Conditions --- Neurologic Degenerative Diseases --- Neurologic Diseases, Degenerative --- Degenerative Diseases, Nervous System --- Degenerative Condition, Neurologic --- Degenerative Conditions, Neurologic --- Degenerative Neurologic Disease --- Degenerative Neurologic Disorder --- Neurodegenerative Disease --- Neurodegenerative Disorder --- Neurologic Degenerative Condition --- Neurologic Degenerative Disease --- Neurologic Disease, Degenerative --- Neurologic Disorder, Degenerative --- Neurologic Disorders, Degenerative --- Luft Disease --- Luft's Disease --- Megaconial Myopathies --- Mitochondrial Myopathy --- Pleoconial Myopathies --- Myopathies, Mitochondrial --- Disease, Luft --- Disease, Luft's --- Lufts Disease --- Megaconial Myopathy --- Myopathies, Pleoconial --- Myopathy, Megaconial --- Myopathy, Pleoconial --- Pleoconial Myopathy --- Mitochondria, Muscle --- Mitochondrion --- Mitochondrial Contraction --- Contraction, Mitochondrial --- Contractions, Mitochondrial --- Mitochondrial Contractions --- Subcellular Space --- Intracellular Spaces --- Space, Intracellular --- Space, Subcellular --- Spaces, Intracellular --- Spaces, Subcellular --- Subcellular Spaces --- Anatomies --- Nutritional Physiological Phenomena --- Protoplasm --- Cytoplasms --- Protoplasms --- Organelle --- Fraction, Subcellular --- Fractions, Subcellular --- Subcellular Fraction --- Cell Fractionation --- Myopathic Conditions --- Muscle Disorders --- Myopathies --- Muscle Disorder --- Muscular Disease --- Myopathic Condition --- Myopathy --- Mitochondrial Disorders --- Mitochondrial Electron Transport Chain Deficiencies --- Mitochondrial Respiratory Chain Deficiencies --- Electron Transport Chain Deficiencies, Mitochondrial --- Oxidative Phosphorylation Deficiencies --- Respiratory Chain Deficiencies, Mitochondrial --- Deficiencies, Oxidative Phosphorylation --- Deficiencies, Respiratory Chain --- Deficiency, Oxidative Phosphorylation --- Deficiency, Respiratory Chain --- Disease, Mitochondrial --- Disorder, Mitochondrial --- Disorders, Mitochondrial --- Mitochondrial Disease --- Mitochondrial Disorder --- Oxidative Phosphorylation Deficiency --- Phosphorylation Deficiencies, Oxidative --- Phosphorylation Deficiency, Oxidative --- Respiratory Chain Deficiency --- Nervous System Disorders --- Neurological Disorders --- Neurologic Disorders --- Disease, Nervous System --- Diseases, Nervous System --- Disorder, Nervous System --- Disorder, Neurologic --- Disorder, Neurological --- Disorders, Nervous System --- Disorders, Neurologic --- Disorders, Neurological --- Nervous System Disease --- Nervous System Disorder --- Neurologic Disorder --- Neurological Disorder --- Neurology --- Cell --- Cell Biology --- Cell Components --- Cell Component --- Cellular Structure --- Component, Cell --- Components, Cell --- Structure, Cellular --- Structures, Cellular --- Orthopedic Disorders --- Musculoskeletal Disease --- Orthopedic Disorder --- Cramp-Fasciculation Syndrome --- Fasciculation-Cramp Syndrome, Benign --- Foley-Denny-Brown Syndrome --- Oppenheim's Disease --- Amyotonia Congenita --- Oppenheim Disease --- Benign Fasciculation-Cramp Syndrome --- Benign Fasciculation-Cramp Syndromes --- Cramp Fasciculation Syndrome --- Cramp-Fasciculation Syndromes --- Fasciculation Cramp Syndrome, Benign --- Fasciculation-Cramp Syndromes, Benign --- Foley Denny Brown Syndrome --- Neuromuscular Disease --- Oppenheims Disease --- Syndrome, Cramp-Fasciculation --- Syndrome, Foley-Denny-Brown --- Syndromes, Cramp-Fasciculation --- Cytoplasmic Structure --- Structure, Cytoplasmic --- Structures, Cytoplasmic --- Diseases, Metabolic --- Thesaurismosis --- Disease, Metabolic --- Metabolic Disease --- Thesaurismoses