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SILAC : Methods and Protocols
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ISBN: 1071628623 1071628631 Year: 2023 Publisher: New York, NY : Springer US : Imprint: Humana,

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Abstract

This detailed volume explores the most recent methodologies and applications of stable isotope labeling by amino acids in cell culture (SILAC). With the development of new technologies in the field of mass spectrometry and bioinformatics, SILAC-based methods have become the first tool of choice in quantitative proteomics either on its own or in combination with other more recently developed approaches, which is elucidated in the chapters of this book. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, SILAC: Methods and Protocols serves as an ideal guide for researchers working in the area of functional proteomics and other aspects of protein science.


Book
Subcellular components: preparation and fractionation
Author:
ISBN: 0839105819 0408703601 9780839105817 9780408703604 Year: 1972 Publisher: London : Butterworths,

Chromosome techniques : theory and practice.
Authors: ---
ISBN: 0839106289 0408703075 9780839106289 9780408703079 Year: 1972 Publisher: London Butterworths

Cytochalasins, biochemical and cell biological aspects
Author:
ISBN: 072040651X 9780720406511 Year: 1978 Volume: 46 Publisher: Amsterdam North-Holland


Book
Fetal Stem Cells in Regenerative Medicine : Principles and Translational Strategies
Authors: ---
ISBN: 1493934813 149393483X Year: 2016 Publisher: New York, NY : Springer New York : Imprint: Springer,

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This book explores the regenerative properties of fetal stem cells, from feto-maternal cell traffic through perinatal stem cells, with a discussion of key topics including stem cell banking, drug screening, in utero stem cell transplantation and ethical considerations. The expertly authored chapters also delve into embryonic, amniotic membrane, and umbilical cord blood stem cells; fetal development models; fetal cell reprogramming; culture methods; disease models; perinatal gene therapy, and more. These chapters are grouped into four sections, each discussing a separate prenatal stem cell population and providing fascinating historical contexts for our knowledge of these systems. Featuring a foreword written by the renowned Dr. Joseph Vacanti of the Harvard Stem Cell Institute, Fetal Stem Cells in Regenerative Medicine: Principles and Translational Strategies is a welcome and timely contribution to the Stem Cell Biology and Regenerative Medicine series. It is essential reading for scientists and researchers, clinicians and residents, and advanced students involved in stem cells, regenerative medicine, tissue engineering, and related disciplines such as embryology.


Book
Molecular Genetic Approaches to Maize Improvement
Authors: ---
ISBN: 3540689192 9786611876210 1281876216 3540689222 Year: 2009 Publisher: Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer,

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During the past decade, there has been tremendous progress in maize biotechnology. This volume provides an overview of our current knowledge of maize molecular genetics, how it is being used to improve the crop, and future possibilities for crop enhancement. Several chapters deal with genetically engineered traits that are currently, or soon will be, in commercial production. Technical approaches for introducing novel genes into the maize genome, the regeneration of plants from transformed cells, and the creation of transgenic lines for field production are covered. Further, the authors describe how molecular genetic techniques are being used to identify genes and characterize their function, and how these procedures are utilized to develop elite maize germplasm. Moreover, molecular biology and physiological studies of corn as a basis for the improvement of its nutritional and food-making properties are included. Finally, the growing use of corn as biomass for energy production is discussed.


Book
Cell and Tissue Reaction Engineering
Authors: --- --- --- ---
ISBN: 3540681752 3642018742 3540681825 Year: 2009 Publisher: Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer,

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Abstract

The completion of the Human Genome Project and the rapid progress in cell biology and biochemical engineering, are major forces driving the steady increase of approved biotech products, especially biopharmaceuticals, in the market. Today mammalian cell products (“products from cells”), primarily monoclonals, cytokines, recombinant glycoproteins, and, increasingly, vaccines, dominate the biopharmaceutical industry. Moreover, a small number of products consisting of in vitro cultivated cells (“cells as product”) for regenerative medicine have also been introduced in the market. Their efficient production requires comprehensive knowledge of biological as well as biochemical mammalian cell culture fundamentals (e.g., cell characteristics and metabolism, cell line establishment, culture medium optimization) and related engineering principles (e.g., bioreactor design, process scale-up and optimization). In addition, new developments focusing on cell line development, animal-free culture media, disposables and the implications of changing processes (multi-purpose- facilities) have to be taken into account. While a number of excellent books treating the basic methods and applications of mammalian cell culture technology have been published, only little attention has been afforded to their engineering aspects. The aim of this book is to make a contribution to closing this gap; it particularly focuses on the interactions between biological and biochemical and engineering principles in processes derived from cell cultures. It is not intended to give a comprehensive overview of the literature. This has been done extensively elsewhere.

Keywords

Cell culture -- Industrial applications. --- Cell Culture Techniques. --- Cells. --- Cytological Techniques. --- Genetic Techniques. --- Mammals -- Cytology -- Technique. --- Medical informatics. --- Molecular Biology. --- Tissue engineering. --- Tissue engineering --- Culture Techniques --- Clinical Laboratory Techniques --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Tissue Engineering --- Biology --- Health & Biological Sciences --- Cytology --- Biomedical Engineering --- Life sciences. --- Pharmaceutical technology. --- Biotechnology. --- Biochemical engineering. --- Cell biology. --- Cell culture. --- Biomedical engineering. --- Life Sciences. --- Cell Culture. --- Biochemical Engineering. --- Biomedical Engineering. --- Pharmaceutical Sciences/Technology. --- Cell Biology. --- Biomedical engineering --- Regenerative medicine --- Tissue culture --- Cytology. --- Biomedical Engineering and Bioengineering. --- Cell biology --- Cellular biology --- Cells --- Cytologists --- Pharmaceutical laboratory techniques --- Pharmaceutical laboratory technology --- Technology, Pharmaceutical --- Technology --- Clinical engineering --- Medical engineering --- Bioengineering --- Biophysics --- Engineering --- Medicine --- Chemical engineering --- Genetic engineering --- Bio-process engineering --- Bioprocess engineering --- Biochemistry --- Biotechnology --- Cultures (Biology) --- Technique --- Pharmaceutical chemistry. --- Chemical Bioengineering. --- Pharmaceutics. --- Technique. --- Chemistry, Medical and pharmaceutical --- Chemistry, Pharmaceutical --- Drug chemistry --- Drugs --- Medical chemistry --- Medicinal chemistry --- Pharmacochemistry --- Chemistry


Book
Hepatobiliary and Pancreatic Carcinogenesis in the Hamster
Authors: --- ---
ISBN: 443187772X 9786611986926 1281986925 4431877738 Year: 2009 Publisher: Tokyo : Springer Japan : Imprint: Springer,

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Abstract

Malignant neoplasms occurring in the biliary tract and pancreas remain a therap- tic challenge. The mechanism of carcinogenesis as well as the growth and spread of these tumors is still poorly understood, making the development of rational tre- ment strategies difficult. In order to improve the clinical results achieved by sur- cal or other medical treatment of such malignant tumors, the establishment of an experimental animal model is critical. For this purpose, attempts were made to induce carcinoma experimentally in the biliary tree and finally an animal model using the hamster was established in 1994 at our laboratory. Because the tumor in this model mimicked the characteristics of human tumors, a series of experimental investigations were conducted to clarify the pathological characteristics of biliary carcinoma, the genetic alterations during biliary carcinogenesis, and the relationship between biliary inflammation and c- cinogenesis. The chemopreventive effects on the occurrence of biliary carcinoma were also successfully examined. In addition, in vitro studies led to the establi- ment of transplantable biliary cancer cell lines and biliary epithelial cell lines by utilizing the hamster model. This monograph represents the collective efforts in hepato-biliary and pancreatic disease research over the past 20 years. I hope that this monograph will be a source of useful knowledge for basic researchers as well as for clinicians involved in the care of patients with hepato-biliary and pancreatic neoplasms. Takashi Kanematsu, M.D., Ph.D.

Keywords

Animal Physiology. --- Cancer -- Animal models. --- Cytology -- Technique. --- Biliary Tract Neoplasms --- Pancreatic Neoplasms --- Cricetinae --- Liver Neoplasms --- Models, Animal --- Carcinoma --- Pancreatic Diseases --- Digestive System Neoplasms --- Muridae --- Endocrine Gland Neoplasms --- Neoplasms, Glandular and Epithelial --- Investigative Techniques --- Liver Diseases --- Biliary Tract Diseases --- Endocrine System Diseases --- Digestive System Diseases --- Neoplasms by Histologic Type --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Neoplasms by Site --- Rodentia --- Diseases --- Neoplasms --- Mammals --- Vertebrates --- Chordata --- Animals --- Eukaryota --- Organisms --- Oncology --- Gastroenterology --- Medicine --- Health & Biological Sciences --- Liver --- Biliary tract --- Pancreas --- Animal models. --- Biliary tree --- Medicine. --- Cancer research. --- Hepatology. --- Surgical oncology. --- Animal anatomy. --- Animal physiology. --- Biology --- Biomedicine. --- Cancer Research. --- Biological Techniques. --- Animal Anatomy / Morphology / Histology. --- Surgical Oncology. --- Technique. --- Digestive organs --- Endocrine glands --- Exocrine glands --- Bile --- Abdomen --- Oncology. --- Cytology --- Morphology (Animals). --- Clinical medicine. --- Research --- Methodology. --- Cancer --- Oncologic surgery --- Oncological surgery --- Surgical oncology --- Medicine, Clinical --- Animal physiology --- Anatomy --- Animal morphology --- Body form in animals --- Zoology --- Morphology --- Cell biology --- Cellular biology --- Cells --- Cytologists --- Tumors --- Excision --- Treatment --- Physiology --- Biology—Technique. --- Animal anatomy --- Cancer research

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