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This detailed volume explores the most recent methodologies and applications of stable isotope labeling by amino acids in cell culture (SILAC). With the development of new technologies in the field of mass spectrometry and bioinformatics, SILAC-based methods have become the first tool of choice in quantitative proteomics either on its own or in combination with other more recently developed approaches, which is elucidated in the chapters of this book. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, SILAC: Methods and Protocols serves as an ideal guide for researchers working in the area of functional proteomics and other aspects of protein science.
Proteins. --- Cytology—Technique. --- Cytological Techniques. --- Proteids --- Biomolecules --- Polypeptides --- Proteomics
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Biological techniques --- Histology. Cytology --- Cell fractionation --- Cytology --- Subcellular fractionation --- Congresses --- Technique --- Subcellular fractionation. --- Congresses. --- Monograph --- Cell fractionation - Congresses --- Cytology - Technique - Congresses
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Biological techniques --- Histology. Cytology --- Chromosomes --- Cytological Technics --- Cytology --- Cytologie --- Technique --- CYTOLOGICAL TECHNIQUES --- CHROMOSOMES --- chromosomes --- Code génétique --- genetic code --- Chromosomes. --- Cytological Techniques. --- 575'3 --- 576.316 --- 57.086 --- Cytologic Technics --- Cytological Technic --- Cytological Technique --- Technic, Cytological --- Technics, Cytological --- Technique, Cytological --- Techniques, Cytological --- Cytologic Technic --- Technic, Cytologic --- Technics, Cytologic --- Cell Biology --- Chromosome --- methods --- Cytology - Technique --- Basic Sciences. Genetics --- Cytogenetics --- Cytology - Technique. --- Cytogenetics.
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Biological techniques --- Histology. Cytology --- Chromosomes --- Cytology --- Technique --- Chromosomes. --- -Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Chromosome theory --- Cell nuclei --- Crossing over (Genetics) --- Cytotaxonomy --- Genetics --- Karyokinesis --- Linkage (Genetics) --- Chromosome --- ZV anatomy & fysiology --- Offprints --- Technique. --- -Technique --- Cytology - Technique
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Cytochalasins --- Cytology --- Physiological effect --- Technique --- -Cytology --- -Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Antibiotics --- Heterocyclic compounds --- Mycotoxins --- Physiological effects --- Cytochalasins. --- Physiological effect. --- Technique. --- -Physiological effects --- Cytochalasins - Physiological effect --- Cytology - Technique
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Histology. Cytology --- Microelectrodes --- Electrodes, Ion selective --- Cytology --- Cytological Techniques --- Technique --- Cytological Techniques. --- -Electrodes, Ion selective --- Miniature electrodes --- Electrodes --- Ion selective electrodes --- Ion sensitive electrodes --- Specific ion electrodes --- Electrochemical analysis --- Ion-permeable membranes --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Cytologic Technics --- Cytological Technic --- Cytological Technics --- Cytological Technique --- Technic, Cytological --- Technics, Cytological --- Technique, Cytological --- Techniques, Cytological --- Cytologic Technic --- Technic, Cytologic --- Technics, Cytologic --- Cell Biology --- methods --- Electrodes, Ion selective. --- Microelectrodes. --- Technique. --- Electrodes. --- Cytology - Technique
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This book explores the regenerative properties of fetal stem cells, from feto-maternal cell traffic through perinatal stem cells, with a discussion of key topics including stem cell banking, drug screening, in utero stem cell transplantation and ethical considerations. The expertly authored chapters also delve into embryonic, amniotic membrane, and umbilical cord blood stem cells; fetal development models; fetal cell reprogramming; culture methods; disease models; perinatal gene therapy, and more. These chapters are grouped into four sections, each discussing a separate prenatal stem cell population and providing fascinating historical contexts for our knowledge of these systems. Featuring a foreword written by the renowned Dr. Joseph Vacanti of the Harvard Stem Cell Institute, Fetal Stem Cells in Regenerative Medicine: Principles and Translational Strategies is a welcome and timely contribution to the Stem Cell Biology and Regenerative Medicine series. It is essential reading for scientists and researchers, clinicians and residents, and advanced students involved in stem cells, regenerative medicine, tissue engineering, and related disciplines such as embryology.
Life sciences. --- Stem cells. --- Cell culture. --- Regenerative medicine. --- Tissue engineering. --- Embryology. --- Life Sciences. --- Stem Cells. --- Regenerative Medicine/Tissue Engineering. --- Cell Culture. --- Stem cells --- Therapeutic use. --- Colony-forming units (Cells) --- Mother cells --- Progenitor cells --- Medicine --- Regeneration (Biology) --- Cells --- Animal embryology --- Animals --- Development, Embryological --- Development, Embryonic --- Development, Zygotic --- Embryogenesis --- Embryogeny --- Embryological development --- Embryonic development --- Zoology --- Zygote development --- Zygotes --- Zygotic development --- Zygotic embryogenesis --- Developmental biology --- Morphology (Animals) --- Embryos --- Reproduction --- Cultures (Biology) --- Cytology --- Embryology --- Development --- Technique --- Biomedical engineering --- Regenerative medicine --- Tissue culture --- Cytology—Technique. --- Stem Cell Biology. --- Regenerative Medicine and Tissue Engineering. --- Cytological Techniques.
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During the past decade, there has been tremendous progress in maize biotechnology. This volume provides an overview of our current knowledge of maize molecular genetics, how it is being used to improve the crop, and future possibilities for crop enhancement. Several chapters deal with genetically engineered traits that are currently, or soon will be, in commercial production. Technical approaches for introducing novel genes into the maize genome, the regeneration of plants from transformed cells, and the creation of transgenic lines for field production are covered. Further, the authors describe how molecular genetic techniques are being used to identify genes and characterize their function, and how these procedures are utilized to develop elite maize germplasm. Moreover, molecular biology and physiological studies of corn as a basis for the improvement of its nutritional and food-making properties are included. Finally, the growing use of corn as biomass for energy production is discussed.
Corn. --- Crop improvement. --- Agriculture --- Earth & Environmental Sciences --- Agriculture - General --- Plant Sciences --- Corn --- Molecular genetics. --- Crops --- Improvement, Crop --- Improvement --- Life sciences. --- Genetic engineering. --- Food --- Agriculture. --- Cell culture. --- Plant genetics. --- Plant breeding. --- Life Sciences. --- Plant Breeding/Biotechnology. --- Plant Genetics & Genomics. --- Genetic Engineering. --- Cell Culture. --- Food Science. --- Biotechnology. --- Genetics --- Molecular aspects --- Food science. --- Plant Genetics and Genomics. --- Farming --- Husbandry --- Industrial arts --- Life sciences --- Food supply --- Land use, Rural --- Science --- Cultures (Biology) --- Cytology --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Plants --- Breeding --- Technique --- Food—Biotechnology. --- Plant biotechnology. --- Cytology—Technique. --- Plant Biotechnology. --- Plant Genetics. --- Chemical Bioengineering. --- Cytological Techniques. --- Food technology --- Chemical engineering --- Genetic engineering --- Crop biotechnology --- Agricultural biotechnology
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The completion of the Human Genome Project and the rapid progress in cell biology and biochemical engineering, are major forces driving the steady increase of approved biotech products, especially biopharmaceuticals, in the market. Today mammalian cell products (“products from cells”), primarily monoclonals, cytokines, recombinant glycoproteins, and, increasingly, vaccines, dominate the biopharmaceutical industry. Moreover, a small number of products consisting of in vitro cultivated cells (“cells as product”) for regenerative medicine have also been introduced in the market. Their efficient production requires comprehensive knowledge of biological as well as biochemical mammalian cell culture fundamentals (e.g., cell characteristics and metabolism, cell line establishment, culture medium optimization) and related engineering principles (e.g., bioreactor design, process scale-up and optimization). In addition, new developments focusing on cell line development, animal-free culture media, disposables and the implications of changing processes (multi-purpose- facilities) have to be taken into account. While a number of excellent books treating the basic methods and applications of mammalian cell culture technology have been published, only little attention has been afforded to their engineering aspects. The aim of this book is to make a contribution to closing this gap; it particularly focuses on the interactions between biological and biochemical and engineering principles in processes derived from cell cultures. It is not intended to give a comprehensive overview of the literature. This has been done extensively elsewhere.
Cell culture -- Industrial applications. --- Cell Culture Techniques. --- Cells. --- Cytological Techniques. --- Genetic Techniques. --- Mammals -- Cytology -- Technique. --- Medical informatics. --- Molecular Biology. --- Tissue engineering. --- Tissue engineering --- Culture Techniques --- Clinical Laboratory Techniques --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Tissue Engineering --- Biology --- Health & Biological Sciences --- Cytology --- Biomedical Engineering --- Life sciences. --- Pharmaceutical technology. --- Biotechnology. --- Biochemical engineering. --- Cell biology. --- Cell culture. --- Biomedical engineering. --- Life Sciences. --- Cell Culture. --- Biochemical Engineering. --- Biomedical Engineering. --- Pharmaceutical Sciences/Technology. --- Cell Biology. --- Biomedical engineering --- Regenerative medicine --- Tissue culture --- Cytology. --- Biomedical Engineering and Bioengineering. --- Cell biology --- Cellular biology --- Cells --- Cytologists --- Pharmaceutical laboratory techniques --- Pharmaceutical laboratory technology --- Technology, Pharmaceutical --- Technology --- Clinical engineering --- Medical engineering --- Bioengineering --- Biophysics --- Engineering --- Medicine --- Chemical engineering --- Genetic engineering --- Bio-process engineering --- Bioprocess engineering --- Biochemistry --- Biotechnology --- Cultures (Biology) --- Technique --- Pharmaceutical chemistry. --- Chemical Bioengineering. --- Pharmaceutics. --- Technique. --- Chemistry, Medical and pharmaceutical --- Chemistry, Pharmaceutical --- Drug chemistry --- Drugs --- Medical chemistry --- Medicinal chemistry --- Pharmacochemistry --- Chemistry
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Malignant neoplasms occurring in the biliary tract and pancreas remain a therap- tic challenge. The mechanism of carcinogenesis as well as the growth and spread of these tumors is still poorly understood, making the development of rational tre- ment strategies difficult. In order to improve the clinical results achieved by sur- cal or other medical treatment of such malignant tumors, the establishment of an experimental animal model is critical. For this purpose, attempts were made to induce carcinoma experimentally in the biliary tree and finally an animal model using the hamster was established in 1994 at our laboratory. Because the tumor in this model mimicked the characteristics of human tumors, a series of experimental investigations were conducted to clarify the pathological characteristics of biliary carcinoma, the genetic alterations during biliary carcinogenesis, and the relationship between biliary inflammation and c- cinogenesis. The chemopreventive effects on the occurrence of biliary carcinoma were also successfully examined. In addition, in vitro studies led to the establi- ment of transplantable biliary cancer cell lines and biliary epithelial cell lines by utilizing the hamster model. This monograph represents the collective efforts in hepato-biliary and pancreatic disease research over the past 20 years. I hope that this monograph will be a source of useful knowledge for basic researchers as well as for clinicians involved in the care of patients with hepato-biliary and pancreatic neoplasms. Takashi Kanematsu, M.D., Ph.D.
Animal Physiology. --- Cancer -- Animal models. --- Cytology -- Technique. --- Biliary Tract Neoplasms --- Pancreatic Neoplasms --- Cricetinae --- Liver Neoplasms --- Models, Animal --- Carcinoma --- Pancreatic Diseases --- Digestive System Neoplasms --- Muridae --- Endocrine Gland Neoplasms --- Neoplasms, Glandular and Epithelial --- Investigative Techniques --- Liver Diseases --- Biliary Tract Diseases --- Endocrine System Diseases --- Digestive System Diseases --- Neoplasms by Histologic Type --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Neoplasms by Site --- Rodentia --- Diseases --- Neoplasms --- Mammals --- Vertebrates --- Chordata --- Animals --- Eukaryota --- Organisms --- Oncology --- Gastroenterology --- Medicine --- Health & Biological Sciences --- Liver --- Biliary tract --- Pancreas --- Animal models. --- Biliary tree --- Medicine. --- Cancer research. --- Hepatology. --- Surgical oncology. --- Animal anatomy. --- Animal physiology. --- Biology --- Biomedicine. --- Cancer Research. --- Biological Techniques. --- Animal Anatomy / Morphology / Histology. --- Surgical Oncology. --- Technique. --- Digestive organs --- Endocrine glands --- Exocrine glands --- Bile --- Abdomen --- Oncology. --- Cytology --- Morphology (Animals). --- Clinical medicine. --- Research --- Methodology. --- Cancer --- Oncologic surgery --- Oncological surgery --- Surgical oncology --- Medicine, Clinical --- Animal physiology --- Anatomy --- Animal morphology --- Body form in animals --- Zoology --- Morphology --- Cell biology --- Cellular biology --- Cells --- Cytologists --- Tumors --- Excision --- Treatment --- Physiology --- Biology—Technique. --- Animal anatomy --- Cancer research
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