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Cysteine proteinases --- Cysteine proteinases --- Cysteine --- Endopeptidases --- Protease Inhibitors --- Congresses --- Inhibitors --- Congresses --- antagonists & inhibitors

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In Calpain Methods and Protocols, John S. Elce and a seasoned team of principal investigators present a set of proven and easily followed protocols for studying calpain. The methods include in vitro techniques for the detection, expression, purification, and assay of µ- and m-calpain, supplemented with a wide range of system and tissue models for studying both the physiological functions and the effects of inhibitors on calpain. The systems used include neural tissue, kidney, liver, the eye, and membrane fusion in muscle and erythrocytes, each in connection with hypoxia or other injury. Among the analytical techniques employed are casein zymography, immunofluorescence, and calpain activity assays. The authors also examine specific substrates that have been proposed for the calpains. Highly practical and readily repeatable, Calpain Methods and Protocols offers investigators involved in basic and clinically oriented calpain research a gold-standard collection of powerful experimental tools for discovering the nature and function of calpains.

Calpain --- Cysteine proteinases. --- Proteinase --- Calcium-dependent cysteine proteinase --- Cysteine proteinases --- Neurosciences. --- Neural sciences --- Neurological sciences --- Neuroscience --- Medical sciences --- Nervous system

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Cysteine proteases expressed by pathogenic organisms play key roles in virulence including host entry, feeding and suppression of host immune responses. This book gives comprehensive coverage to all aspects of pathogen cysteine proteases and brings together numerous scientific advances which have been made over many years. Thus, the biochemistry, molecular biology and structure‑function relationships of these important pathogen enzymes are covered in detail. Written by leading researchers from Europe, Australia and North America, this book is essential reading for students and professionals interested in human medicine and infectious disease research.

Cysteine proteinases -- Pathophysiology. --- Microbial enzymes. --- Pathogenic microorganisms. --- Cysteine proteinases --- Microbial enzymes --- Pathogenic microorganisms --- Natural Science Disciplines --- Organisms --- Peptide Hydrolases --- Hydrolases --- Disciplines and Occupations --- Enzymes --- Enzymes and Coenzymes --- Chemicals and Drugs --- Chemistry --- Bacteria --- Eukaryota --- Viruses --- Cysteine Proteases --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- Pathophysiology --- Pathophysiology. --- Disease-causing microorganisms --- Micro-organisms, Pathogenic --- Pathogens --- Medicine. --- Molecular biology. --- Biomedicine. --- Biomedicine general. --- Molecular Medicine. --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biophysics --- Biomolecules --- Systems biology --- Clinical sciences --- Medical profession --- Human biology --- Life sciences --- Medical sciences --- Pathology --- Physicians --- Microorganisms --- Medical microbiology --- Virulence (Microbiology) --- Microbiological chemistry --- Proteinase --- Health Workforce --- Biomedicine, general.

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Enzymology --- Oncology. Neoplasms --- Endopeptidases --- Neoplasm Invasiveness --- Metastasis --- Proteinase --- Cancer invasiveness --- Métastases --- Cancer --- Caractère envahissant --- Lymphatic Metastasis. --- Cysteine Proteases. --- Endopeptidases. --- Peptide Peptidohydrolases --- Cysteine Protease --- Cysteine Proteinase --- Cysteine Proteinases --- Protease, Cysteine --- Proteases, Cysteine --- Proteinase, Cysteine --- Proteinases, Cysteine --- Lymphatic Metastases --- Metastases, Lymphatic --- Metastasis, Lymphatic --- Sentinel Lymph Node --- Sentinel Lymph Node Biopsy --- diagnosis. --- Conferences - Meetings --- Métastases --- Caractère envahissant --- Cysteine Proteases --- Lymphatic Metastasis --- Proteolytic enzymes --- Dissemination of cancer --- Invasiveness (Oncology) --- Neoplasm invasiveness --- Spread of cancer --- Tumor dissemination --- Tumor invasiveness --- Tumor progression --- Tumor spread --- Cancer cells --- Congresses --- diagnosis --- Growth --- Lymph Node Metastasis --- Lymph Node Metastases --- Metastasis, Lymph Node --- Endopeptidase