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Mesenchymal Stem Cells have seen an unprecedented level of interest in the last decade, primarily due to their relative ease of isolation, the large numbers of cells present in the adult, and the ability to propagate these cells in culture. In Mesenchymal Stem Cell Assays and Applications, expert researchers from across the globe explore the latest techniques to propagate, characterize, and engineer this special cell type. Chapters outline a set of protocols and assays used by leading investigators in the field, providing standards that can be applied by all researchers to the population of cells used in their experiments. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Ground-breaking and current, Mesenchymal Stem Cell Assays and Applications is a necessary handbook for all researchers working with this ambiguous population of cells.

Cytology. --- Stem cells. --- Cell Biology. --- Stem Cells. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Colony-forming units (Cells) --- Mother cells --- Progenitor cells --- Mesenchymal Stem Cells --- Cell Culture Techniques. --- Cells, Cultured. --- Cytological Techniques. --- Multipotent Stem Cells --- Stromal Cells. --- cytology. --- Cell, Stromal --- Cells, Stromal --- Stromal Cell --- Cytologic Technics --- Cytological Technic --- Cytological Technics --- Cytological Technique --- Technic, Cytological --- Technics, Cytological --- Technique, Cytological --- Techniques, Cytological --- Cytologic Technic --- Technic, Cytologic --- Technics, Cytologic --- Cell Biology --- Cultured Cells --- Cell, Cultured --- Cultured Cell --- Organ Culture Techniques --- Coculture Techniques --- Cell Culture Techniques --- Culture Techniques --- Human Umbilical Vein Endothelial Cells --- Cell Culture --- Cell Culture Technique --- Cell Cultures --- Culture Technique, Cell --- Culture Techniques, Cell --- Cells, Cultured --- methods --- Cytology

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Anne Kantardjieff and Weichang Zhou Mammalian Cell Cultures for Biologics Manufacturing Scott Estes and Mark Melville Mammalian Cell Line Developments in Speed and Efficiency Susan Abu-Absi, Sen Xu, Hugh Graham, Nimish Dalal, Marcus Boyer and Kedar Dave Cell Culture Process Operations for Recombinant Protein Production Sadettin S. Ozturk Equipment for Large-Scale Mammalian Cell Culture Daniel M. Marasco, Jinxin Gao, Kristi Griffiths, Christopher Froggatt, Tongtong Wang and Gan Wei Development and Characterization of a Cell Culture Manufacturing Process Using Quality by Design (QbD) Principles Michael J. Gramer Product Quality Considerations for Mammalian Cell Culture Process Development and Manufacturing Dayue Chen Safety Assurance for Biologics Manufactured in Mammalian Cell Cultures: A Multitiered Strategy Dawn M. Ecker and Thomas C. Ransohoff Mammalian Cell Culture Capacity for Biopharmaceutical Manufacturing Karthik P. Jayapal and Chetan T. Goudar Transcriptomics as a Tool for Assessing the Scalability of Mammalian Cell Perfusion Systems Enda Moran and Patrick Gammell Lifecycle Management for Recombinant Protein Production Using Mammalian Cell Culture Technology.

Chemistry. --- Pharmaceutical technology. --- Biotechnology. --- Cell culture. --- Cell Culture. --- Pharmaceutical Sciences/Technology. --- Biologicals --- Cell culture --- Vertebrates --- Proteins --- Complex Mixtures --- Culture Techniques --- Amino Acids, Peptides, and Proteins --- Chemicals and Drugs --- Chordata --- Clinical Laboratory Techniques --- Investigative Techniques --- Animals --- Eukaryota --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Organisms --- Cell Culture Techniques --- Biological Products --- Mammals --- Recombinant Proteins --- Mechanical Engineering --- Engineering & Applied Sciences --- Bioengineering --- Proteins, Biosynthetic --- Proteins, Recombinant DNA --- Biosynthetic Proteins --- DNA Recombinant Proteins --- DNA Proteins, Recombinant --- Proteins, DNA Recombinant --- Proteins, Recombinant --- Recombinant DNA Proteins --- Recombinant Proteins, DNA --- Mammalia --- Mammal --- Biologic Products --- Products, Biological --- Biologics --- Natural Products --- Products, Biologic --- Products, Natural --- Cell Culture --- Cell Culture Technique --- Cell Cultures --- Culture Technique, Cell --- Culture Techniques, Cell --- Eucarya --- Eukarya --- Eukaryotes --- Eukaryotas --- Eukaryote --- Animalia --- Animal --- Metazoa --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Diagnoses and Laboratory Examinations --- Diagnosis, Laboratory --- Laboratory Diagnosis --- Laboratory Examinations and Diagnoses --- Laboratory Techniques, Clinical --- Clinical Laboratory Technique --- Diagnoses, Laboratory --- Laboratory Diagnoses --- Laboratory Technique, Clinical --- Technique, Clinical Laboratory --- Techniques, Clinical Laboratory --- Chordates --- Chordate --- Culture Technique --- Technique, Culture --- Techniques, Culture --- Complex Extracts --- Crude Extracts --- Extracts, Complex --- Extracts, Crude --- Mixtures, Complex --- Gene Products, Protein --- Gene Proteins --- Protein Gene Products --- Proteins, Gene --- Vertebrate --- Pharmaceutical laboratory techniques --- Pharmaceutical laboratory technology --- Technology, Pharmaceutical --- Biologicals. --- Cultures (Biology) --- Cytology --- Technique --- Biological products --- Medical supplies --- Technology --- Chemical engineering --- Genetic engineering --- Chemistry --- Pharmaceutical technology --- Biotechnology

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This book represents an essential reference manual for all of the well-characterized leukemia-lymphoma cell lines currently available. It provides the most important facts, using the succinct and user-friendly format that has made the FactsBooks so popular with scientists and clinical researchers. Introductory chapters provide background and perspective for culturing malignant hematopoietic (blood forming) cell lines. These chapters are followed by over 400 comprehensive individual entries. Each cell line entry highlights essential clinical, immunological, genetic, and functional featur

Cancer cells. --- Cell Line. --- Cell lines. --- Hematologic Neoplasms. --- Hematopoietic stem cells. --- Human cell culture. --- Leukemia. --- Leukemia, Experimental. --- Leukemia, Experimental--Handbooks, manuals, etc. Lymphomas--Handbooks, manuals, etc. Cancer cells--Handbooks, manuals, etc. Human cell culture--Handbooks, manuals, etc. Hematopoietic stem cells--Handb. --- Lymphoma. --- Lymphomas. --- Research. --- Tumor Cells, Cultured. --- Neoplasms by Histologic Type --- Lymphoproliferative Disorders --- Cells, Cultured --- Culture Techniques --- Neoplasms --- Lymphatic Diseases --- Cells --- Clinical Laboratory Techniques --- Immunoproliferative Disorders --- Anatomy --- Diseases --- Investigative Techniques --- Immune System Diseases --- Hemic and Lymphatic Diseases --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Cell Culture Techniques --- Leukemia --- Lymphoma --- Tumor Cells, Cultured --- Medicine --- Health & Biological Sciences --- Hematologic Diseases --- Blood and Lymphatic System Disorders --- Diseases of Immune System --- Immune Diseases --- Immunological Diseases --- Immune Disorders --- Immune System Disorders --- Immunologic Diseases --- Disease, Immune --- Disease, Immune System --- Disease, Immunologic --- Disease, Immunological --- Disorder, Immune System --- Immune Disease --- Immune Disorder --- Immune System Disease --- Immune System Disorder --- Immunologic Disease --- Immunological Disease --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Anatomies --- Disorder, Immunoproliferative --- Disorders, Immunoproliferative --- Immunoproliferative Disorder --- Clinical Laboratory Diagnoses --- Clinical Laboratory Testings --- Clinical Laboratory Tests --- Diagnoses and Laboratory Examinations --- Diagnosis, Laboratory --- Laboratory Diagnosis --- Laboratory Examinations and Diagnoses --- Laboratory Techniques, Clinical --- Clinical Laboratory Technique --- Clinical Laboratory Test --- Clinical Laboratory Testing --- Diagnose, Clinical Laboratory --- Laboratory Diagnoses --- Laboratory Technique, Clinical --- Laboratory Test, Clinical --- Laboratory Testing, Clinical --- Technique, Clinical Laboratory --- Test, Clinical Laboratory --- Testing, Clinical Laboratory --- Laboratories --- Medical Laboratory Science --- Clinical Laboratory Services --- Cell --- Cell Biology --- Lymphatism --- Status Lymphaticus --- Disease, Lymphatic --- Diseases, Lymphatic --- Lymphatic Disease --- Benign Neoplasms --- Malignancy --- Malignant Neoplasms --- Neoplasia --- Neoplasm --- Neoplasms, Benign --- Cancer --- Tumors --- Benign Neoplasm --- Cancers --- Malignancies --- Malignant Neoplasm --- Neoplasias --- Neoplasm, Benign --- Neoplasm, Malignant --- Neoplasms, Malignant --- Tumor --- Medical Oncology --- Culture Technique --- Technique, Culture --- Techniques, Culture --- Diffusion Chambers, Culture --- Cultured Cells --- Cell, Cultured --- Cultured Cell --- Organ Culture Techniques --- Coculture Techniques --- Human Umbilical Vein Endothelial Cells --- Duncan Disease --- Epstein-Barr Virus Infection, Familial Fatal --- Epstein-Barr Virus-Induced Lymphoproliferative Disease In Males --- Familial Fatal Epstein-Barr Infection --- Immunodeficiency 5 --- Immunodeficiency, X-Linked Progressive Combined Variable --- Lymphoproliferative Disease, X-Linked --- Lymphoproliferative Syndrome, X-Linked, 1 --- Purtilo Syndrome --- X-Linked Lymphoproliferative Disease --- X-Linked Lymphoproliferative Disorder --- Duncan's Syndrome --- X-Linked Lymphoproliferative Syndrome --- Disease, Duncan --- Disease, X-Linked Lymphoproliferative --- Diseases, X-Linked Lymphoproliferative --- Disorder, Lymphoproliferative --- Disorder, X-Linked Lymphoproliferative --- Disorders, Lymphoproliferative --- Disorders, X-Linked Lymphoproliferative --- Epstein Barr Virus Induced Lymphoproliferative Disease In Males --- Epstein Barr Virus Infection, Familial Fatal --- Familial Fatal Epstein Barr Infection --- Immunodeficiency 5s --- Immunodeficiency, X Linked Progressive Combined Variable --- Lymphoproliferative Disease, X Linked --- Lymphoproliferative Diseases, X-Linked --- Lymphoproliferative Disorder --- Lymphoproliferative Disorder, X-Linked --- Lymphoproliferative Disorders, X-Linked --- Lymphoproliferative Syndrome, X-Linked --- Lymphoproliferative Syndromes, X-Linked --- Purtilo Syndromes --- Syndrome, Purtilo --- Syndrome, X-Linked Lymphoproliferative --- Syndromes, Purtilo --- Syndromes, X-Linked Lymphoproliferative --- X Linked Lymphoproliferative Disease --- X Linked Lymphoproliferative Disorder --- X Linked Lymphoproliferative Syndrome --- X-Linked Lymphoproliferative Diseases --- X-Linked Lymphoproliferative Disorders --- X-Linked Lymphoproliferative Syndromes --- Amyloidosis --- Histological Type of Neoplasm --- Histological Types of Neoplasms --- Neoplasms by Histological Type --- Neoplasm Histological Type --- Neoplasm Histological Types --- Neoplasms Histological Type --- Neoplasms Histological Types --- Cultured Neoplastic Cells --- Cultured Tumor Cells --- Neoplastic Cells, Cultured --- Cell, Cultured Neoplastic --- Cell, Cultured Tumor --- Cells, Cultured Neoplastic --- Cells, Cultured Tumor --- Cultured Neoplastic Cell --- Cultured Tumor Cell --- Neoplastic Cell, Cultured --- Tumor Cell, Cultured --- Germinoblastoma --- Lymphoma, Malignant --- Reticulolymphosarcoma --- Sarcoma, Germinoblastic --- Germinoblastic Sarcoma --- Germinoblastic Sarcomas --- Germinoblastomas --- Lymphomas --- Lymphomas, Malignant --- Malignant Lymphoma --- Malignant Lymphomas --- Reticulolymphosarcomas --- Sarcomas, Germinoblastic --- Hematologic Neoplasms --- Leucocythaemia --- Leucocythemia --- Leucocythaemias --- Leucocythemias --- Leukemias --- Myeloproliferative Disorders --- Cell Culture --- Cell Culture Technique --- Cell Cultures --- Culture Technique, Cell --- Culture Techniques, Cell