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This detailed book collects original protocols aimed at encouraging and stimulating the scientific community to design and produce models for the laboratory that mimic cell guidance conditions as they occur in vivo. The protocols collected describe powerful strategies to exploit chemical cues involved in cell differentiation processes. Special emphasis is given to the use of methods for purification and characterization of exosomes and other secreted vesicles, as well as micro and non-coding RNAs, that have been demonstrated to control the tuning of the in vivo micro and macro environment in order to ensure the optimal soluble environment in vitro. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Next Generation Culture Platforms for Reliable In Vitro Models: Methods and Protocols serves as an ideal guide for researchers working toward developing these vital testing models for preclinical studies.

Cultiu de teixits --- Cultiu de teixits in vitro --- Teixits (Histologia) --- Enginyeria de teixits --- Transformació cel·lular --- Cell differentiation. --- Regenerative medicine. --- Cell Differentiation. --- Regenerative Medicine and Tissue Engineering. --- Medicine --- Regeneration (Biology) --- Cell fate specification --- Cell specification --- Cells --- Differentiation of cells --- Fate specification of cells --- Specification of cells --- Morphogenesis --- Differentiation --- Fate specification --- Specification --- Diferenciació cel·lular --- Citodiferenciació --- Diferenciació de les cèl·lules --- Histogènesi --- Morfogènesi --- Genètica del desenvolupament

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Cardiovascular diseases are still the leading cause of death in developed countries. Revascularization procedures such as coronary artery and peripheral bypass grafts, as well as access surgery represent a 2$ billion market yearly for the US alone. Despite intense research over many decades, no clinically suitable, shelf-ready, synthetic, vascular, small-caliber graft exists. There is therefore still a quest for such a clinical vascular prosthesis for surgical revascularization procedures and access surgery. Many approaches have been tried and are currently under investigation with promising results. These range from acellular and cell-based, stable or bio-degradable, synthetic scaffolds to biological or decellularized grafts, not forgetting self-assembly technologies for in vitro or in vivo VTE. All these approaches can be further enhanced by functionalization, e.g. with growth factors and drug elution. This updatable book aims to cover all the relevant aspects of Vascular Tissue Engineering (VTE) and novel alternatives to develop vascular grafts for clinical applications. The chapters in this book cover different aspects of manufacturing scaffolds with various polymers, mechanical characteristics, degradation rates, decellularization techniques, cell sheet assembly, 3-D printing and autologous mandril-based VTE. All the necessary in vitro tests such as biocompatibility and thrombogenicity are reviewed. Pre-clinical assessment of in vivo experimental models include patency, compliance, intimal hyperplasia, inflammatory reaction, cellular ingrowth and remodeling. Finally, early clinical trials will be periodically updated regarding results, regulatory aspects and post-marketing quality assessment. Furthermore, the reader should get an insight into various approaches, technologies and methods to better understand the complexity of blood surface and cell interactions in VTE. Translational research has yielded early human applications clearly showing the enormous need of research in the field to provide better solutions for our patients and this continuously updated book will hopefully become a reference in the field for life sciences.

Regenerative medicine. --- Tissue engineering. --- Biomedical engineering. --- Surgery. --- Biomaterials. --- Regenerative Medicine/Tissue Engineering. --- Biomedical Engineering and Bioengineering. --- Surgery, Primitive --- Medicine --- Clinical engineering --- Medical engineering --- Bioengineering --- Biophysics --- Engineering --- Biomedical engineering --- Regenerative medicine --- Tissue culture --- Regeneration (Biology) --- Biocompatible materials --- Biomaterials --- Medical materials --- Materials --- Biocompatibility --- Prosthesis --- Vascular grafts. --- Blood-vessels --- Transplantation of organs, tissues, etc. --- Grafts --- Transplantation --- Bioartificial materials --- Hemocompatible materials --- Biomaterials (Biomedical materials) --- Biomedical materials. --- Enginyeria de teixits --- Medicina regenerativa --- Medicina --- Regeneració (Biologia) --- Enginyeria tisular --- Reconstrucció d'òrgans --- Reconstrucció de teixits --- Cultiu de teixits --- Enginyeria biomèdica

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Biomedical materials. --- Regenerative medicine. --- Tissue engineering. --- Biomedical engineering --- Regenerative medicine --- Tissue culture --- Medicine --- Regeneration (Biology) --- Bioartificial materials --- Biocompatible materials --- Biomaterials (Biomedical materials) --- Hemocompatible materials --- Medical materials --- Materials --- Biocompatibility --- Prosthesis --- Materials biomèdics --- Medicina regenerativa --- Enginyeria de teixits --- Enginyeria tisular --- Reconstrucció d'òrgans --- Reconstrucció de teixits --- Cultiu de teixits --- Enginyeria biomèdica --- Medicina --- Regeneració (Biologia) --- Biomaterials --- Materials biocompatibles --- Materials mèdics --- Materials en medicina --- Biocompatibilitat --- Col·loides en medicina --- Materials dentals --- Polímers en medicina --- Pròtesis

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Tissue engineering --- Tissue culture --- Génie tissulaire --- Tissus (Histologie) --- Periodicals --- Périodiques --- Culture --- Tissue Engineering. --- Tissue engineering. --- Engineering, Tissue --- stem cells --- progenitor cells --- Histology. --- Biological Engineering. --- Tissues --- Tissues. --- Biomedical engineering --- Regenerative medicine --- Histology --- Organs (Anatomy) --- Organ Culture Techniques --- Tissue Expansion --- Regenerative Medicine --- Guided Tissue Regeneration --- Cell- and Tissue-Based Therapy --- General biochemistry --- Medicina regenerativa. --- Enginyeria de teixits --- Enginyeria biomèdica. --- Enginyeria clínica --- Enginyeria mèdica --- Bioenginyeria --- Biofísica --- Enginyeria --- Medicina --- Electrònica mèdica --- Materials biomèdics --- Aparells i instruments mèdics --- Enginyeria tisular --- Reconstrucció d'òrgans --- Reconstrucció de teixits --- Cultiu de teixits --- Enginyeria biomèdica --- Medicina regenerativa --- Regeneració (Biologia) --- Microphysiological Systems

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This book presents cutting-edge papers and perspectives on the transport of oxygen to tissues by scientists in a multitude of disciplines such as biochemistry, engineering, mathematics, medicine, physics, physiology, veterinary and complementary medicine. The book is composed of the following 6 parts: Brain Oxygenation and Function, Tumor Oxygenation and Metabolism, Muscle Oxygenation and Sports Medicine, Cell Metabolism and Tissue Oxygenation, Methodology of O2 Measurements, and Special Topics. The articles in this book have been presented at the 42nd annual meeting of the International Society on Oxygen Transport to Tissue (ISOTT 2019) held in Albuquerque, New Mexico, USA, from July 28 to July 31, 2019. Academics, clinical and industry researchers, engineers, as well as graduate students who are interested in oxygen transport to tissue will find this book a great reference and a useful learning resource. The chapters "Near-Infrared Spectroscopy Measured Cerebral Blood Flow from Spontaneous Oxygenation Changes in Neonatal Brain Injury", "Developing a Model to Simulate the Effect of Hypothermia on Cerebral Blood Flow and Metabolism" and "Broadband NIRS Cerebral Evaluation of the Hemodynamic and Oxidative State of Cytochrome-c-Oxidase Responses to +Gz Acceleration in Healthy Volunteers" are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Medicine. --- Biomedicine, general. --- Health Workforce --- Oxygen --- Tissue respiration --- Physiological transport --- Physiological transport. --- Delivery of oxygen (Physiology) --- Oxygen delivery (Physiology) --- Oxygen transport (Physiology) --- Chalcogens --- Nonmetals --- Photosynthetic oxygen evolution --- Oxigen en l'organisme --- Teixits (Histologia) --- Fisiologia humana --- Biologia humana --- Ciències de la salut --- Fisiologia --- Mecànica humana --- Reproducció humana --- Cos humà --- Teixits (Biologia) --- Teixits animals --- Teixits humans --- Teixits orgànics --- Cèl·lules i teixits vegetals --- Cultiu de teixits --- Epiteli --- Músculs --- Periodonci --- Teixit nerviós --- Teixit connectiu --- Cèl·lules animals --- Histologia --- Empelts de teixits --- Òrgans (Anatomia) --- Composició del cos humà --- Oximetria --- Anoxèmia --- Medicine --- Biology --- Biomedical Research. --- Research. --- Biological research --- Biomedical research --- Oxygen,