Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

The ability to transfer and maintain DNA both within and between species is an essential skill in biotechnology and medicine. In Bacterial Artificial Chromosomes, expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1: Library Construction, Physical Mapping, and Sequencing presents readily reproducible techniques for library construction, physical mapping, and sequencing. The laboratory protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies. An accompanying volume, Volume 2: Functional Studies, provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. Comprehensive and cutting-edge, the two volumes of Bacterial Artificial Chromosomes provide a superlative collection of highly productive protocols that will prove useful to many bioscientists, including genome sequencers, geneticists, molecular biologists, and biochemists studying the structure and function of specific genomes.

Bacterial artificial chromosomes -- Laboratory manuals. --- Bacterial chromosomes. --- Electronic books. -- local. --- Biology --- Cytology --- Health & Biological Sciences --- Bacterial artificial chromosomes --- Gene Library --- Genetic Techniques --- Chromosomes, Artificial --- Investigative Techniques --- Chromosomes --- Genetic Structures --- Chromosome Mapping --- Chromosomes, Artificial, Bacterial --- Cloning, Molecular --- Genomic Library --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Cellular Structures --- Genetic Phenomena --- Phenomena and Processes --- Cells --- Anatomy --- BACs (Chromosomes) --- Artificial chromosomes --- Bacterial chromosomes --- Bacteriology. --- Microbiology. --- Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Microbial biology --- Microorganisms --- Microbiology --- Composition --- Chromosome mapping --- Chromosomes, artificial, bacterial --- Cloning, molecular --- Genetic structures --- Genomic library

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Yeast artificial chromosomes (YACs) have their origins in the molecular genetic analysis of the yeast Saccharomyces cerevisiae. The construction of self-maintaining genetic elements from isolated frag­ ments of the yeast genome defined DNA sequences necessary for chro­ mosome function has provided telomeres, centromeres, and autonomous replicating sequences. In 1987 a reversal of the strategy put these short functional DNA sequences to work in cloning vectors, producing "yeast" chromosomes largely composed of foreign DNA. Initially the insert size of clones averaged several hundred kilobasepairs, a remarkable achieve­ ment. Rapid progress with cloning technology has since enabled the construction of YAC libraries with average insert sizes of around 1 Mb, with many clones exceeding that size, and YACs remain the largest capacity microbiological cloning system available. They effectively bridge the size gap between bacterial cloning (plasmids, cosmids, PI, and bacterial artificial chromosomes) and what could be considered mammalian cloning systems (somatic cell hybrids and irradiati- fusion gene transfer hybrids). YACs also brought with them a conceptual revolution in the man­ agement of clone libraries. The large carrying capacity of YACs, with subsequent reduction in the total number required, meant that it was conceivable to store clones individually rather than as pools that require constant re-plating. Each clone in the library has a unique address and, with successive screenings, information accumulates about individual clones.

Chromosomes, Artificial, Yeast --- Genetic Vectors --- Genetic Techniques --- Molecular cloning --- Genetic vectors --- Yeast fungi --- Saccharomyces cerevisiae --- Vecteurs de clonage --- Methodology --- Genetic engineering --- Genetic vectors. --- Saccharomyces cerevisiae. --- Methodology. --- Genetic engineering. --- Saccharomyces --- Yeast genetic engineering --- Microbial genetic engineering --- Cloning vectors --- Expression vectors (Genetics) --- rDNA vectors --- Recombinant DNA vectors --- Vectors, Genetic --- Gene expression --- Recombinant DNA --- Cloning, Molecular --- DNA cloning --- Gene cloning --- Cloning --- Molecular genetics --- Clone cells --- Human genetics. --- Human Genetics. --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology