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Epigenetics and DNA Damage, a new volume in the Translational Epigenetics series, offers a thorough grounding in the relationship between DNA Damage, epigenetic modifications, and chromatin regulation. Early chapters address the basic science of DNA damage and its association with various epigenetic mechanisms, including DNA methylation, post-translational histone modifications, histone variants, chromatin remodeling, miRNAs, and lncRNAs. This is followed by a close discussion of DNA damage and epigenetics in metabolism, aging, cellular differentiation, immune function, stem cell biology, and cancer, tying recent research to translational application in disease understanding. Later chapters examine possible epigenetic therapies combining DNA damage induction and epigenetic alteration, as well as instructive chapters on how to analyze DNA damage and epigenetic alterations in new research.

Epigenetics. --- DNA damage. --- Damage, DNA --- Biochemical genetics --- Mutation (Biology) --- Genetics --- DNA Damage --- Chromatin Assembly and Disassembly --- Epigenesis, Genetic --- genetics --- genetics (DNLM)D042002Q000235 --- Epigenesis, Genetic. --- genetics. --- genetics (DNLM)D042002Q000235.

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Recent advances in epigenetic research as well as the development of exciting new technologies have helped greatly in unraveling the many mysteries of nontraditional genetic processes. In Epigenetics Protocols, hands-on researchers describe state-of-the-art methods for epigenetic analysis, including recent breakthrough techniques that have great potential in the rapidly expanding field of non-Mendelian genetics. The authors provide techniques for the analysis of chromatin remodeling, such as histone acetylation and methylation. In addition, methods in newly developed and especially promising areas of epigenetics, such as telomere position effects, quantitative epigenetics, and ADP ribosylation are covered. There is also an updated analysis of techniques involving DNA methylation and its role in the modification, as well as the maintenance, of chromatin structure. Of special interest are potentially revolutionary techniques. These include methods for determining changes in native chromatin, methods of microarray analysis applied to epigenetics, and methylation-sensitive single-strand conformation techniques. The methods are suitable not only for studying fundamental biological processes, but also for investigating possible therapeutic interventions and such diseases as cancer. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and easy to use, Epigenetics Protocols offers investigators readily reproducible techniques that will further promote progress in this critically important field.

Human genetics. --- Human Genetics. --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology --- Chromatin Assembly and Disassembly --- Chromosome Structures --- DNA Methylation. --- Epigenesis, Genetic. --- Histone Code --- Histones --- genetics. --- metabolism. --- Epigenetic Process --- Epigenetics Processes --- Epigenetic Processes --- Genetic Epigenesis --- Process, Epigenetic --- Processes, Epigenetic --- Processes, Epigenetics --- Epigenome --- DNA Methylation --- DNA Methylations --- Methylation, DNA --- Methylations, DNA --- Methyl CpG Binding Domain --- Genomic Imprinting --- Gene Silencing --- Epigenesis, Genetic

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This book is designed for advanced students and researchers in cell biology, biochemistry, molecular biology, medicine in general, and cancer in particular. It provides the latest data on the transcriptome of the mammary gland in order to establish the molecular and cellular biology of differentiation leading to cancer prevention. The authors have based their work on the epidemiological evidence that early first full term pregnancy is a protective factor in humans against breast cancer and using this knowledge have developed in vivo and in vitro experimental systems that have demonstrated mechanistically how the differentiation takes place.  The transcriptoma analysis of the female breast shows that an early first full term pregnancy reprograms the organ by imprinting a genomic signature that differs according to reproductive history.  This reprogramming takes place at the chromatin level by changing the transcriptional process. The modification of the transcriptional control is due to the expression of non-coding RNA sequences and post-transcriptional control driven by the spliceosome. The plasticity of the genome of the human breast makes possible this reprogramming that is not only induced by the physiological process of pregnancy but by the use of hormones mimicking pregnancy. The role of stem cells and their reprogramming during differentiation are presented as a new paradigm in breast cancer prevention.

Antineoplastic agents -- Side effects. --- Breast -- Cancer -- Psychological aspects. --- Breast -- Cancer -- Radiotherapy. --- Breast -- Cancer -- Treatment -- Complications. --- Breast --- Medicine --- Oncology --- Toxicology --- Breast Diseases --- Gene Expression Regulation --- DNA Packaging --- RNA --- Neoplasms by Site --- Biology --- Neoplasms --- Cell Physiological Processes --- Nucleic Acids --- Genetic Processes --- Skin Diseases --- Biological Science Disciplines --- Genetic Phenomena --- Nucleic Acids, Nucleotides, and Nucleosides --- Cell Physiological Phenomena --- Natural Science Disciplines --- Diseases --- Skin and Connective Tissue Diseases --- Phenomena and Processes --- Chemicals and Drugs --- Disciplines and Occupations --- Breast Neoplasms --- Chromatin Assembly and Disassembly --- RNA, Untranslated --- Genetics --- Health & Biological Sciences --- Cancer --- Prevention. --- Medicine. --- Cancer research. --- Pharmacology. --- Biomedicine. --- Cancer Research. --- Pharmacology/Toxicology.

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This volume includes timely reviews of several aspects of chromatin biology written by scientists at the forefront of this rapidly moving field. Topics covered include the structure and function of protein modules within chromatin-remodeling proteins, newly characterized histone modifications (methylation, ubiquitylation) and their functional consequences, transcription and histone dynamics, roles of chromatin remodeling factors in DNA replication and repair, and current models of nucleosome-remodeling mechanisms.

Chromatin Assembly and Disassembly. --- Cell Physiology. --- Histones. --- Chromatin. --- Chromatine --- Life sciences. --- Biochemistry. --- Cytology. --- Life Sciences. --- Cell Biology. --- Biochemistry, general. --- Chromatin --- Histones --- Chromatin Assembly and Disassembly --- Cell Physiological Phenomena --- DNA Packaging --- Gene Expression Regulation --- Nuclear Proteins --- Nucleoproteins --- Chromosome Structures --- Phenomena and Processes --- Proteins --- Genetic Structures --- Genetic Processes --- Cell Physiological Processes --- Chromosomes --- Amino Acids, Peptides, and Proteins --- Intranuclear Space --- Genetic Phenomena --- Chemicals and Drugs --- Cell Nucleus Structures --- Cell Nucleus --- Intracellular Space --- Cellular Structures --- Cells --- Anatomy --- Cytology --- Biology --- Health & Biological Sciences --- Biosciences --- Sciences, Life --- Anatomies --- Cell --- Cell Components --- Cell Component --- Cellular Structure --- Component, Cell --- Components, Cell --- Structure, Cellular --- Structures, Cellular --- Cell Nuclei --- Nuclei, Cell --- Nucleus, Cell --- Cell Nucleus Structure --- Structure, Cell Nucleus --- Structures, Cell Nucleus --- Genetic Concepts --- Genetic Phenomenon --- Genetic Process --- Concept, Genetic --- Concepts, Genetic --- Genetic Concept --- Phenomena, Genetic --- Phenomenon, Genetic --- Process, Genetic --- Processes, Genetic --- Chromosome --- Cell Physiological Phenomenon --- Cell Physiological Process --- Physiology, Cell --- Cell Physiology --- Phenomena, Cell Physiological --- Phenomenon, Cell Physiological --- Physiological Process, Cell --- Physiological Processes, Cell --- Process, Cell Physiological --- Processes, Cell Physiological --- Genetic Structure --- Structure, Genetic --- Structures, Genetic --- Gene Products, Protein --- Gene Proteins --- Protein Gene Products --- Proteins, Gene --- Chromosome Structure --- Structure, Chromosome --- Structures, Chromosome --- Nucleoprotein --- Nuclear Protein --- Nucleolar Proteins --- Protein, Nuclear --- Proteins, Nuclear --- Proteins, Nucleolar --- Expression Regulation, Gene --- Regulation, Gene Action --- Regulation, Gene Expression --- Gene Action Regulation --- Regulation of Gene Expression --- Packaging, DNA --- Chromatin Assembly --- Chromatin Disassembly --- Chromatin Modeling --- Chromatin Remodeling --- Chromatin Disassemblies --- Disassembly, Chromatin --- Remodeling, Chromatin --- Chromatins --- Histone --- Histone H1 --- Histone H1(s) --- Histone H2a --- Histone H2b --- Histone H3 --- Histone H3.3 --- Histone H4 --- Histone H5 --- Histone H7 --- Cell biology --- Cellular biology --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- physiology --- Composition --- Subcellular Space --- Intracellular Spaces --- Space, Intracellular --- Space, Subcellular --- Spaces, Intracellular --- Spaces, Subcellular --- Subcellular Spaces --- Subnuclear Space --- Intranuclear Spaces --- Space, Intranuclear --- Space, Subnuclear --- Spaces, Intranuclear --- Spaces, Subnuclear --- Subnuclear Spaces --- Cell biology. --- Cytologists --- Chemistry --- Medical sciences