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Embryonic stem (ES) cells have significant potential in basic studies designed to better understand how different cells and tissues in the body are formed, as well as for generating unlimited numbers of cells for transplantation, drug delivery, and drug testing. In Embryonic Stem Cells: Methods and Protocols, Kursad Turksen and a panel of international experts describe their most productive methods for using ES cells as in vitro developmental models for many cell and tissue types. Set out in step-by-step detail by the investigators who developed them, these protocols range widely from ES cell isolation, maintenance, and modulation of gene expression, to cutting-edge techniques that use cDNA arrays in gene expression analysis and phage display libraries. There are also advanced techniques for the generation of antibodies against very rare antigens and for the identification and characterization of proteins and protein interactions. Additional studies of the ES cell cycle and apoptosis, as well as protocols for the use of ES cells to generate diverse cell and tissue types, complete this collection of readily reproducible methods. Many of the techniques have already been shown to have tremendous utility with ES cells and their differentiated progeny. Authoritative and state-of-the-art, this unique first collection of protocols for the study of ES cells, Embryonic Stem Cells: Methods and Protocols, will prove an invaluable resource not only for those generally interested in cell and developmental biology, but also for those actively using, or planning to use, ES cells to study fate choices and specific lineages.

Human histology. Human cytology --- Analytical biochemistry --- Embryonic stem cells --- Cellules souches embryonnaires --- Laboratory manuals --- Manuels de laboratoire --- Embryology, Experimental. --- Experimental embryology --- Embryonal stem cells --- Stem cells --- Embryology --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists

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Embryonic stem cells --- Biotechnology --- Cellules souches embryonnaires --- Biotechnologie --- Research --- Law and legislation --- Patents. --- Moral and ethical aspects --- Recherche --- Droit --- Brevets d'invention --- Aspect moral --- Embryonal stem cells --- Stem cells --- Patents --- Moral and ethical aspects. --- Government policy --- European Parliament. --- Council of the European Union. --- Moral and ethical aspects"">Moral and ethical aspects --- Research&delete& --- Patents&delete&

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Gene targeting --- Cell Line --- Genetic Engineering --- Genetic Vectors --- Mice, Transgenic --- Mutation --- Stem Cells --- Embryonic stem cells --- Genetic engineering --- Genetic vectors --- Transgenic mice --- Cellules souches embryonnaires --- Génie génétique --- Vecteurs de clonage --- Souris transgéniques --- laboratory manuals --- genetics --- laboratory manuals.

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Stem cell science has the potential to impact human reproductive medicine significantly - cutting edge technologies allow the production and regeneration of viable gametes from human stem cells offering potential to preciously infertile patients. Written by leading experts in the field Stem Cells in Reproductive Medicine brings together chapters on the genetics and epigenetics of both the male and female gametes as well as advice on the production and regeneration of gene cells in men and women, trophoblasts and endometrium from human embryonic and adult stem cells. Although focussing mainly on the practical elements of the use of stem cells in reproductive medicine, the book also contains a section on new developments in stem cell research. The book is essential reading for reproductive medicine clinicians, gynecologists and embryologists who want to keep abreast of practical developments in this rapidly developing field.

Adult Stem Cells. --- Embryonic Stem Cells. --- Genitalia --- Reproductive Medicine --- Reproductive Techniques, Assisted. --- Embryonic stem cells. --- Reproductive health. --- Human reproductive technology. --- Cellules souches embryonnaires --- Santé de la reproduction --- Procréation médicalement assistée --- cytology. --- methods. --- Assisted conception --- Assisted human reproduction --- Assisted human reproductive technology --- Conception --- Human assisted reproduction --- Human assisted reproductive technology --- Human reproduction --- Medical technology --- Reproductive technology --- Human reproductive health --- Human reproductive medicine --- Reproductive medicine --- Health --- Embryonal stem cells --- Stem cells --- Technological innovations --- Health aspects

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Quel chemin suivre pour un questionnement adéquat, éclairé dans sa dimension médicale, morale et juridique et conforme à la conscience de l’un et de l’autre acteur de cette décision à prendre ? Si vous vous posez une question qui concerne le début ou la fin de la vie, des techniques de pointe en transplantation, en génétique ou en recherche médicale, vous trouverez chez cet auteur , qui a confronté ses vues avec ses collègues de plusieurs disciplines de la Commission d’éthique de l’UCL, des pistes de réflexion sérieuses et respectueuses de la conscience d’un chacun.

Surrogate mothers --- Human reproductive technology --- Medicine --- Medical ethics --- Bioethics --- Medical sciences --- Euthanasia --- Hospice care --- Patients --- Embryonic stem cells --- Mères porteuses --- Procréation médicalement assistée --- Médecine --- Ethique médicale --- Bioéthique --- Sciences de la santé --- Euthanasie --- Soins en phase terminale --- Cellules souches embryonnaires --- Legal status, laws, etc. --- Law and legislation --- Research --- Moral and ethical aspects --- Droit --- Recherche --- Aspect moral --- Ethics, Medical --- bio-ethiek (medische, biomedische ethiek, bio-ethische aspecten) --- recht (wetgeving, rechtspraak, rechtsbeginselen, juridische aspecten, aansprakelijkheid) --- patiëntenrechten (rechten van de patiënt) --- reproductief recht (recht om zich voort te planten, recht op een kind) --- commissie voor medische ethiek (ethisch comité, CME) --- bioéthique (éthique médicale, biomédicale, aspects bioéthiques) --- droit (aspects juridiques, législation, jurisprudence, principes de droit, responsabilité) --- droits du patient (droits des malades) --- droit reproductif (droit de procréer, droit à la procréation, droits de la reproduction, droit à l'enfant) --- comité d'éthique médicale (comité d'éthique, CEM) --- Mères porteuses --- Procréation médicalement assistée --- Médecine --- Ethique médicale --- Bioéthique --- Sciences de la santé --- Belgium --- Droit médical --- Transplantation (chirurgie) --- Génétique --- Consentement éclairé (droit médical) --- Personnel médical --- Belgique --- Responsabilité professionnelle