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The RTgill-W1 cell line assay describes a 24-well plate format fish cell line acute toxicity test using the permanent cell line from rainbow trout (Oncorhynchus mykiss) gill, RTgill-W1. After 24 h of exposure to the test chemical, cell viability is assessed based on three fluorescent cell viability indicator dyes, measured on the same set of cells. Resazurin enters the cells in its non-fluorescent form and is converted to the fluorescent product, resorufin, by mitochondrial, microsomal or cytoplasmic oxidoreductases. A reduction in the fluorescence of resorufin indicates a decline in cellular metabolic activity, including disruption of mitochondrial membranes. The data are expressed as the percent cell viability of unexposed control values versus the test chemical concentration. The resulting concentration-response curves serve to determine the effective concentrations causing 50% loss in cell viability, i.e. the EC50 value. The test is designed to (i) predict fish acute toxicity in product testing; (ii) range-finding and pre-screening before conducting a full fish acute or other fish-based toxicity test; (iii) generation of toxicity information to be used for hazard assessment in combination with other lines of evidences (e.g., Quantitative Structure Activity Relationships (QSAR), weight of evidence (WoE)) within Integrated Testing Strategy (ITS)/Integrated Approach to Testing and Assessment (IATA).
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Cell Lineage and Fate Determination provides a comprehensive view of the mechanisms regulating cell lineage and fate determination in an effort to understand how the fertilized egg is transformed into a complex of specialized tissues. It presents basic information on eight different animal models and recent developmental biological research done in each model. The book provides a focused forum presenting key information for researchers studying various aspects of developmental and cellular biology. Extensive use of tables and black-and-white and color figures helps illustrate each model
Continuous cell lines. --- Cell lines. --- Cell culture --- CCL (Continuous cell lines) --- CCLs (Continuous cell lines) --- Cell lines
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International Review of Cytology presents current advances and comprehensive reviews in cell biology, both plant and animal. Articles address structure and control of gene expression, nucleocytoplasmic interactions, control of cell development and differentiation, and cell transformation and growth. Authored by some of the foremost scientists in the field, each volume provides up-to-date information and directions for future research.This volume brings together current information on the localization and roles of RNAs in cell-lineage determination and subsequent patterning in embryo
Pattern formation (Biology) --- Cell lines. --- Cell lines --- Cell determination --- Embryology --- Cell aggregation --- Developmental cytology
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This special issue of the International Journal of Molecular Sciences contains a collection of articles by colleagues of Antonius (Ton) Gerardus Rolink (19/04/1953-06/08/2017) and honors Ton's life and profound knowledge of and huge contribution to science. Ton participated in an FP7 Marie Curie Initial Training Network called DECIDE, and partners have submitted articles for this Special Issue. Scientists outside this network have also submitted articles. The articles examine various aspects of how the hematopoietic stem-cell gives rise to the different types of blood and immune cells. These include decision-making by the hematopoietic stem cell and the importance of controlling events within cells and the niches the cell resides in. New insights into these processes at the basic scientific level have given rise to an emerging new model for the development of blood cells. In turn, changes to our understanding of this process have led to new and exciting propositions regarding what goes wrong during the early stages of the development of leukemia.
Cell lines. --- Hematopoiesis. --- Blood formation --- Hemopoiesis --- Blood --- Cell culture
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Cell determination --- Cell lines --- Developmental cytology --- Hematopoiesis --- Stem cells
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Cell lines --- Lignées cellulaire --- Catalogs and collections --- Catalogues
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Histology. Cytology --- Neuropathology --- Nerve tissue --- Cell Line. --- Neurons --- Cultures and culture media. --- physiology. --- Cell lines. --- Physiology.
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Cell differentiation --- Cell lines --- Developmental cytology --- Congresses. --- Cell culture --- Congresses --- CELL DIFFERENTIATION --- CELL LINE --- DEVELOPMENTAL BIOLOGY --- CONGRESSES
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