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The RTgill-W1 cell line assay describes a 24-well plate format fish cell line acute toxicity test using the permanent cell line from rainbow trout (Oncorhynchus mykiss) gill, RTgill-W1. After 24 h of exposure to the test chemical, cell viability is assessed based on three fluorescent cell viability indicator dyes, measured on the same set of cells. Resazurin enters the cells in its non-fluorescent form and is converted to the fluorescent product, resorufin, by mitochondrial, microsomal or cytoplasmic oxidoreductases. A reduction in the fluorescence of resorufin indicates a decline in cellular metabolic activity, including disruption of mitochondrial membranes. The data are expressed as the percent cell viability of unexposed control values versus the test chemical concentration. The resulting concentration-response curves serve to determine the effective concentrations causing 50% loss in cell viability, i.e. the EC50 value. The test is designed to (i) predict fish acute toxicity in product testing; (ii) range-finding and pre-screening before conducting a full fish acute or other fish-based toxicity test; (iii) generation of toxicity information to be used for hazard assessment in combination with other lines of evidences (e.g., Quantitative Structure Activity Relationships (QSAR), weight of evidence (WoE)) within Integrated Testing Strategy (ITS)/Integrated Approach to Testing and Assessment (IATA).

Cell lines.

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Continuous cell lines --- Congresses

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International Review of Cytology presents current advances and comprehensive reviews in cell biology, both plant and animal. Articles address structure and control of gene expression, nucleocytoplasmic interactions, control of cell development and differentiation, and cell transformation and growth. Authored by some of the foremost scientists in the field, each volume provides up-to-date information and directions for future research.This volume brings together current information on the localization and roles of RNAs in cell-lineage determination and subsequent patterning in embryo

Pattern formation (Biology) --- Cell lines. --- Cell lines --- Cell determination --- Embryology --- Cell aggregation --- Developmental cytology

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Cell determination --- Cell lines --- Developmental cytology --- Hematopoiesis --- Stem cells

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Histology. Cytology --- Neuropathology --- Nerve tissue --- Cell Line. --- Neurons --- Cultures and culture media. --- physiology. --- Cell lines. --- Physiology.