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Molecular Biology Techniques: A Classroom Laboratory Manual, Fourth Edition is a must-have collection of methods and procedures on how to create a single, continuous, comprehensive project that teaches students basic molecular techniques. It is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology--or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students will gain hands-on experience on subcloning a gene into an expression vector straight through to the purification of the recombinant protein.

Genetics --- Molecular biology --- Genetic Techniques --- Molecular Biology --- CRISPR-Cas Systems --- Technique. --- methods --- Genetic Techniques. --- Biologie moléculaire --- methods. --- Méthodologie. --- Molecular biology.

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Molecular biology --- Genetics --- medische technologie --- CRISPR-Cas Systems. --- CRISPR-Associated Proteins. --- Genetic Techniques. --- RNA --- ARN --- Analysis --- Laboratory manuals --- Analyse --- Manuels de laboratoire --- Genetica --- DNA --- Ribonucleic acid --- Ribose nucleic acid --- Nucleic acids --- Ribose --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Genetic Counseling --- Genetic Phenomena --- methods --- CASCADE Proteins, CRISPR-Associated --- CRISPR-Associated Complex for Antiviral Defense Proteins --- CRISPR-Associated Protein Complex Proteins --- Repeat-Associated Mysterious Proteins --- CASCADE Proteins, CRISPR Associated --- CRISPR Associated Complex for Antiviral Defense Proteins --- CRISPR Associated Protein Complex Proteins --- CRISPR Associated Proteins --- CRISPR-Associated CASCADE Proteins --- Proteins, CRISPR-Associated --- Proteins, Repeat-Associated Mysterious --- Repeat Associated Mysterious Proteins --- Clustered Regularly Interspaced Short Palindromic Repeats --- CRISPR Cas Systems --- CRISPR-Cas System --- System, CRISPR-Cas --- Systems, CRISPR-Cas --- Gene Editing --- CRISPR-Associated Protein 9 --- CRISPR-Cas Systems --- CRISPR-Associated Proteins --- Genetic Techniques --- RNA, Guide, CRISPR-Cas Systems --- Subgenomic RNA

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Gene and Genome Editing will publish methodological and translational research covering the spectrum of gene editing techniques and applications, in any living organism.

Medical genetics --- Genomics --- Medical genetics. --- Genomics. --- Genetics, Medical --- Gene Editing --- Génétique médicale --- Génomique --- Génétique médicale. --- Génomique. --- Clinical genetics --- Diseases --- Heredity of disease --- Human genetics --- Medical sciences --- Pathology --- Genetic disorders --- Genome research --- Genomes --- Molecular genetics --- Genome Editing --- Base Editing --- Editing, Base --- Editing, Gene --- Editing, Genome --- CRISPR-Associated Protein 9 --- Mutagenesis, Site-Directed --- CRISPR-Cas Systems --- Comparative Genomics --- Comparative Genomic --- Genomic, Comparative --- Genomics, Comparative --- Human Genome Project --- Genome --- Medical Genetics --- Anthropology, Physical --- Chromosome Disorders --- Sex Chromosome Disorders --- Genetic Diseases, Inborn --- Molecular Medicine --- Genetic aspects --- Research

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Histology. Cytology --- CRISPR (Genetics) --- Genetics --- Clustered Regularly Interspaced Short Palindromic Repeats. --- Gene Editing. --- Genetic Research. --- Research --- Moral and ethical aspects --- Moral and ethical aspects. --- Research. --- Research, Genetic --- Genome Editing --- Base Editing --- Editing, Base --- Editing, Gene --- Editing, Genome --- CRISPR-Associated Protein 9 --- Mutagenesis, Site-Directed --- CRISPR-Cas Systems --- CRISPR Arrays --- CRISPR Clusters --- CRISPR Elements --- CRISPR Loci --- CRISPR Locus --- CRISPR Sequences --- CRISPR Spacer Sequences --- CRISPR Spacers --- CRISPR-Cas Loci --- CRISPRs --- Clustered Regularly Interspaced Short Palindromic Repeat --- Array, CRISPR --- Arrays, CRISPR --- CRISPR --- CRISPR Array --- CRISPR Cas Loci --- CRISPR Cluster --- CRISPR Element --- CRISPR Sequence --- CRISPR Spacer --- CRISPR Spacer Sequence --- CRISPR-Cas Locus --- Cluster, CRISPR --- Clusters, CRISPR --- Element, CRISPR --- Elements, CRISPR --- Loci, CRISPR --- Loci, CRISPR-Cas --- Locus, CRISPR --- Locus, CRISPR-Cas --- Sequence, CRISPR --- Sequence, CRISPR Spacer --- Sequences, CRISPR --- Sequences, CRISPR Spacer --- Spacer Sequence, CRISPR --- Spacer Sequences, CRISPR --- Spacer, CRISPR --- Spacers, CRISPR --- CRISPR-Associated Proteins --- Genetic research --- Biology --- Embryology --- Mendel's law --- Adaptation (Biology) --- Breeding --- Chromosomes --- Heredity --- Mutation (Biology) --- Variation (Biology) --- Clustered Regularly Interspaced Short Palindromic Repeats (Genetics) --- Immunogenetics --- Nucleotide sequence

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Viruses are microscopic agents that exist worldwide and are present in humans, animals, plants, and other living organisms in which they can cause devastating diseases. However, the advances of biotechnology and next-generation sequencing technologies have accelerated novel virus discovery, identification, sequencing, and manipulation, showing that they present unique characteristics that place them as valuable tools for a wide variety of biotechnological applications. Many applications of viruses have been used for agricultural purposes, namely concerning plant breeding and plant protection. Nevertheless, it is interesting to mention that plants have also many advantages to be used in vaccine production, such as the low cost and low risks they entail, showing once more the versatility of the use of viruses in biotechnology. Although it will obviously never be ignored that viruses are responsible for devastating diseases, it is clear that the more they are studied, the more possibilities they offer to us. They are now on the front line of the most revolutionizing techniques in several fields, providing advances that would not be possible without their existence. In this book there are presented studies that demonstrate the work developed using viruses in biotechnology. These studies were brought by experts that focus on the development and applications of many viruses in several fields, such as agriculture, the pharmaceutical industry, and medicine.

Bacteriophage --- Salmonella --- biocontrol --- comparative genomics --- phage diversity --- grapevine --- apple latent spherical virus vector --- virus-induced flowering --- reduced generation time --- breeding of grapevine --- virus elimination --- Newcastle disease virus --- reverse genetics --- vaccines --- infectious diseases --- cancer --- porcine epidemic diarrhea virus --- VLP --- chemokines --- pig --- vaccine --- SARS-CoV-2 --- COVID-19 --- phages --- CRISPR --- viruses --- prevention --- diagnosis --- treatment --- adeno-associated virus (AAV) vector --- jaagsiekte sheep retrovirus (JSRV) --- LTR --- enhancer --- transduction --- viral vaccines --- cancers --- COVID-19 vaccines --- self-replicating RNA vectors --- DNA-based vaccines --- RNA-based vaccines --- plant virus --- viroid --- viral vector --- virus-induced gene silencing (VIGS) --- CRISPR/Cas9 --- genome editing --- carotenoid biosynthesis --- circular RNA --- infectious bursal disease virus --- immunization --- recombinant Lactococcus lactis --- variant strain --- baculovirus --- insect cells --- bacmid --- Tn7 --- genome stability --- protein expression --- chikungunya virus --- VLPs --- bioreactor --- CRISPR/Cas systems --- viral vectors --- gene editing --- plant genome engineering --- viral resistance --- adeno-associated virus --- AAV --- cancer gene therapy --- prophage --- hydrothermal vent --- Hypnocyclicus thermotrophus --- lytic cassette --- Escherichia coli --- heterologous expression --- codon optimization --- codon harmonization --- expression vectors --- aspect ratio --- VNPs --- TMV --- PVX --- CPMV --- geminivirus --- theranostics --- CRISPR-cas9 --- clodronate --- macrophage --- gene therapy --- gene expression --- nanotechnology