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This volume explores the diversity in progenitor cell biology methods and uniquely describes techniques for isolating, generating, and characterizing progenitor cells from either tissue or embryonic stem cells. The chapters in this book cover topics such as isolating progenitor cells from sources including adipose tissue, blood, bone marrow, ear, gut, heart, pancreatic islets, and Whirton’s jelly; deriving neural, pancreatic, and urothelial precursors from the embryonic stem cells; and culturing isolated islets in vitro to generate progenitor cells via epithelial-to-mesenchymal transition. This book also delves into digital droplet PCR; flow cytometry and cell sorting; mitochondrial assays; calcium ratiometric signaling; and 2D gel electrophoresis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Progenitor Cells: Methods and Protocols is a valuable resource for scientists and researchers interested in learning more about this evolving and diverse field. .

Stem cells. --- Stem Cells. --- Colony-forming units (Cells) --- Mother cells --- Progenitor cells --- Cells --- Cèl·lules mare --- Manuals de laboratori --- Tècniques de laboratori --- Cèl·lules --- Cèl·lules mare embrionàries

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Developmental biology --- Embryology --- Molecular genetics --- Developmental biology. --- Embryology. --- Molecular genetics. --- Biologia del desenvolupament --- Cèl·lules mare --- Regeneració (Biologia) --- Medicina regenerativa --- Biologia del desenvolupament. --- Cèl·lules mare. --- Medicina regenerativa.

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Regenerative medicine. --- Medicine --- Regeneration (Biology) --- Cèl·lules mare --- Medicina regenerativa --- Enginyeria de teixits

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Orthopedics. --- Orthopaedics --- Orthopedia --- Surgery --- Cèl·lules mare --- Ortopèdia --- Cirurgia --- Aparells ortopèdics --- Cirurgia ortopèdica --- Infermeria ortopèdica --- Manipulació (Terapèutica) --- Ortopèdia geriàtrica --- Ortopèdia veterinària --- Malalties dels ossos --- Cèl·lules --- Cèl·lules mare embrionàries

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Cèl·lules mare --- Cèl·lules --- Cèl·lules mare embrionàries --- Stem cells. --- Stem cells --- Regenerative medicine. --- Transplantation. --- Medicine --- Regeneration (Biology) --- Stem cell transplantation --- Cell transplantation --- Colony-forming units (Cells) --- Mother cells --- Progenitor cells --- Cells