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Bacterial genomes.

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Over 500 prokaryotic genomes have been sequenced to date, and thousands more have been planned for the next few years. While these genomic sequence data provide unprecedented opportunities for biologists to study the world of prokaryotes, they also raise extremely challenging issues such as how to decode the rich information encoded in these genomes. This comprehensive volume includes a collection of cohesively written chapters on prokaryotic genomes, their organization and evolution, the information they encode, and the computational approaches needed to derive such information. A comparative

Bacterial genomes --- Computational biology --- Microbial genetics --- Prokaryotes --- Monera --- Procaryotae --- Procaryotes --- Prokaryonta --- Prokaryotae --- Prokaryotic protists --- Microorganisms --- Genetics --- Microbiology --- Biology --- Bioinformatics --- Bacterial genome --- Microbial genomes --- Data processing

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This landmark volume details progress in the fast-changing world of bacterial genomics and it includes contributions from a team of over forty world-renowned authors.

Bacteria --- Pathogens --- Nucleotide sequence --- genomes --- identification. --- identification --- microbial ecology --- Immunological techniques --- Bacterial genetics. --- Bacterial genomes. --- Pathogenic bacteria. --- Genomics. --- Genome research --- Genomes --- Molecular genetics --- Bacteria, Pathogenic --- Disease germs --- Pathogenic microorganisms --- Bacterial diseases --- Medical bacteriology --- Bacterial genome --- Microbial genomes --- Bacteriology --- Microbial genetics --- Research --- Genetics

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Microbial Gene Essentiality: Protocols and Bioinformatics contains a comprehensive collection of experimental and computational strategies and techniques for microbial genome-scale essentiality studies, developed and presented by the leading groups in the field. In addition to wet-lab protocols, the book describes (i) statistical methods essential for planning successful large-scale essentiality screens, as well for data evaluation and analysis; (ii) in-silico prediction of gene essentiality using genome-scale reconstructed metabolic models; and (iii) data integration and comparative analysis in the context of genomic databases. This volume provides researchers with a first-stop guide for choosing the most appropriate strategy for planned essentiality studies. Experimental and computational aspects are equally important in genome-scale gene essentiality analysis, as in all other genomic technologies, and Microbial Gene Essentiality: Protocols and Bioinformatics reflects both of these aspects. All protocols follow the successful Methods in Molecular Biology™ series format, each offering an introduction outlining the principles behind the techniques, step-by-step instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding pitfalls, and are intended for both novice and expert scientists.

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