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Bacterial chromosomes. --- Bacterial genetics. --- DNA. --- DNA --- Bacteria --- Chromosomes --- Recombination, genetic

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Bacteria --- Chromosomes, Bacterial --- Bacterial chromosomes --- Bacterial genetics --- Chromosomes bactériens --- Génétique bactérienne --- genetics --- Bacterial chromosomes. --- Bacterial genetics. --- Chromosomes, Bacterial. --- genetics. --- Genetics. --- Chromosomes bactériens --- Génétique bactérienne --- Bacteria - genetics --- CHROMOSOMES, BACTERIAL --- GENETICS, MICROBIAL --- BACTERIA --- GENETICS

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The ability to transfer and maintain DNA both within and between species is an essential skill in biotechnology and medicine. In Bacterial Artificial Chromosomes, expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1: Library Construction, Physical Mapping, and Sequencing presents readily reproducible techniques for library construction, physical mapping, and sequencing. The laboratory protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies. An accompanying volume, Volume 2: Functional Studies, provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. Comprehensive and cutting-edge, the two volumes of Bacterial Artificial Chromosomes provide a superlative collection of highly productive protocols that will prove useful to many bioscientists, including genome sequencers, geneticists, molecular biologists, and biochemists studying the structure and function of specific genomes.

Bacterial artificial chromosomes -- Laboratory manuals. --- Bacterial chromosomes. --- Electronic books. -- local. --- Biology --- Cytology --- Health & Biological Sciences --- Bacterial artificial chromosomes --- Gene Library --- Genetic Techniques --- Chromosomes, Artificial --- Investigative Techniques --- Chromosomes --- Genetic Structures --- Chromosome Mapping --- Chromosomes, Artificial, Bacterial --- Cloning, Molecular --- Genomic Library --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Cellular Structures --- Genetic Phenomena --- Phenomena and Processes --- Cells --- Anatomy --- BACs (Chromosomes) --- Artificial chromosomes --- Bacterial chromosomes --- Bacteriology. --- Microbiology. --- Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Microbial biology --- Microorganisms --- Microbiology --- Composition --- Chromosome mapping --- Chromosomes, artificial, bacterial --- Cloning, molecular --- Genetic structures --- Genomic library

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Megaplasmids are extrachromosomal genetic elements in the size range of 100 kb and larger. They are found in physiologically and phylogenetically diverse groups of bacteria and archaea. By definition, megaplasmids are not essential for the viability of their hosts under all growth conditions, but paradoxically many megaplasmids carry the genetic information for the defining and characteristic traits of the organism in which they reside. Microbial Megaplasmids reviews our knowledge of the extensively studied representatives, such as the catabolic plasmids of the pseudomonads, the rhizobial Sym plasmids, the Ti plasmids of the genus Agrobacterium and the giant enterobacterial virulence plasmids. It also presents snapshots of more recently discovered megaplasmids. The contribution of megaplasmids to the biology of their hosts is described, highlighting the interactions between megaplasmid and chromosomal genes.

Agriculture. --- Bacterial chromosomes. --- Bacteriology. --- Chromosomes, Bacterial. --- DNA Transposable Elements --genetics. --- DNA, Bacterial. --- Genome, Bacterial. --- Microbial genetics. --- Microbiology. --- Plant diseases. --- Plasmids --genetics. --- Plasmids. --- Microorganisms --- Paragenes --- Genetics --- Life sciences. --- Medical microbiology. --- Microbial genomics. --- Plant pathology. --- Life Sciences. --- Plant Pathology. --- Medical Microbiology. --- Microbial Genetics and Genomics. --- Applied Microbiology. --- Microbiology --- Botany --- Communicable diseases in plants --- Crop diseases --- Crops --- Diseases of plants --- Microbial diseases in plants --- Pathological botany --- Pathology, Vegetable --- Phytopathology --- Plant pathology --- Plants --- Vegetable pathology --- Agricultural pests --- Crop losses --- Diseased plants --- Phytopathogenic microorganisms --- Plant pathologists --- Plant quarantine --- Microbial biology --- Biology --- Genomics --- Microbial genetics --- Farming --- Husbandry --- Industrial arts --- Life sciences --- Food supply --- Land use, Rural --- Biosciences --- Sciences, Life --- Science --- Pathology --- Diseases and pests --- Diseases --- Wounds and injuries --- Cytoplasmic inheritance --- Genetic vectors --- Mobile genetic elements --- Extrachromosomal DNA