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Bacterial Toxins --- Virulence --- Bacteria --- Pathogenicity --- Virulence Factors --- Toxins, Bacterial --- Bacterial Toxin --- Toxin, Bacterial --- pathogenicity

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Shiga toxin-producing Escherichia coli (STEC) and its isolated cytotoxin (Stx), also known as "hamburger E. coli" are continuing sources of significant-and sometime fatal-human and animal infections. In E. coli: Shiga Toxin Methods and Protocols, a multidisciplinary panel of leading experimentalists details the key protocols for the diagnosis and study of STEC and Stx pathogenesis. Described in step-by-step detail, these readily reproducible techniques range from those for the diagnosis and detection of STEC bacteria in patient and animal samples, to those for studying the cellular microbiology of STEC infections, especially host-pathogen interactions and the hemolysin of STEC. There are also protocols for studying the details of Shiga toxin (Stx) biology-from the purification of the toxin to the effects of Stx on various host cell functions-and for exploring STEC-mediated disease in various animal models. Each protocol has been thoroughly tested and optimized to produce robust and successful experimental results, and includes troubleshooting tips, advice on avoiding pitfalls, and alternate methods. Comprehensive and up-to-date, E. coli: Shiga Toxin Methods and Protocols offers bacteriologists and medical microbiologists today's most powerful tools for illuminating the molecular and cellular microbiology of STEC's bacterial pathogenesis, as well as the mechanisms of its toxicity.

Escherichia coli infections --- Verocytotoxins --- Escherichia coli Infections --- Shiga Toxin --- pathology --- analysis --- Shiga-like toxins --- SLT (Bacterial toxin) --- VT (Bacterial toxin) --- Colibacillosis --- Enterotoxins --- Gram-negative bacterial infections --- Microbiology. --- Microbial biology --- Biology --- Microorganisms

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This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact

Science: general issues --- Medical microbiology & virology --- Microbiology (non-medical) --- non-ribosomal peptide --- bacterial toxin --- anticancer activity --- antimicrobial activity --- antibiotic --- bacteriocin --- bacterial enzyme

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Toxine --- Toxins --- Bacteria --- Protéine --- proteins --- Streptococcus --- Choléra --- Cholera --- Clostridium botulinum --- Bacterial Proteins. --- Bacterial Toxins. --- Toxins, Bacterial --- Bacterial Gene Product --- Bacterial Gene Protein --- Bacterial Protein --- Gene Product, Bacterial --- Gene Protein, Bacterial --- Gene Proteins, Bacterial --- Protein, Bacterial --- Proteins, Bacterial --- Bacterial Gene Products --- Bacterial Gene Proteins --- Gene Products, Bacterial --- Cholera. --- Bacterial Toxin --- Toxin, Bacterial --- Bacterial Proteins --- Bacterial Toxins --- Diphterie

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Adp-ribosylation. --- Bacterial toxins --- Industrial microorganisms --- Bacterial toxins. --- Guanine nucleotide regulatory protein. --- Poly adp ribose polymerase. --- Signal transduction. --- Adenosinedifosfaat. --- Biologische membranen. --- Stoftransport. --- Bacterial Toxins --- ADP Ribose Transferases --- Receptor-CD3 Complex, Antigen, T-Cell --- Receptor Cross-Talk --- Feedback, Physiological --- Gasotransmitters --- Toxins, Bacterial --- GTP-Binding Proteins --- Signal Transduction --- Receptor Mediated Signal Transduction --- Signal Transduction Pathways --- Signal Transduction Systems --- Receptor-Mediated Signal Transduction --- Signal Pathways --- Pathway, Signal --- Pathway, Signal Transduction --- Pathways, Signal --- Pathways, Signal Transduction --- Receptor-Mediated Signal Transductions --- Signal Pathway --- Signal Transduction Pathway --- Signal Transduction System --- Signal Transduction, Receptor-Mediated --- Signal Transductions --- Signal Transductions, Receptor-Mediated --- System, Signal Transduction --- Systems, Signal Transduction --- Transduction, Signal --- Transductions, Signal --- Cell Communication --- ADP Ribose Transferase --- ADPRT --- ADPRTs --- ART Transferase --- ART Transferases --- ARTase --- ARTases --- Mono ADP-ribose Transferases --- Mono ADPribose Transferase --- Mono ADPribose Transferases --- Mono(ADP-Ribose) Transferase --- Mono(ADP-Ribosyl)transferase --- Mono(ADPribosyl)transferase --- Mono-ADP-Ribosyltransferase --- MonoADPribosyltransferase --- NAD ADP-Ribosyltransferase --- NAD(+)-L-arginine ADP-D-ribosyltransferase --- NAD-Agmatine ADP-Ribosyltransferase --- NAD-Arginine ADP-Ribosyltransferase --- NADP-ADPRTase --- NADP-Arginine ADP-Ribosyltransferase --- ADP-Ribosyltransferase --- Mono(ADP-Ribose) Transferases --- NAD(P)(+)-Arginine ADP-Ribosyltransferase --- NAD+ ADP-Ribosyltransferase --- ADP Ribosyltransferase --- ADP-Ribosyltransferase, NAD --- ADP-Ribosyltransferase, NAD+ --- ADP-Ribosyltransferase, NAD-Agmatine --- ADP-Ribosyltransferase, NAD-Arginine --- ADP-Ribosyltransferase, NADP-Arginine --- ADP-ribose Transferases, Mono --- ADPribose Transferase, Mono --- ADPribose Transferases, Mono --- Mono ADP Ribosyltransferase --- Mono ADP ribose Transferases --- NAD ADP Ribosyltransferase --- NAD Agmatine ADP Ribosyltransferase --- NAD Arginine ADP Ribosyltransferase --- NAD+ ADP Ribosyltransferase --- NADP ADPRTase --- NADP Arginine ADP Ribosyltransferase --- Ribose Transferase, ADP --- Ribose Transferases, ADP --- Transferase, ADP Ribose --- Transferase, ART --- Transferase, Mono ADPribose --- Transferases, ADP Ribose --- Transferases, ART --- Transferases, Mono ADP-ribose --- Transferases, Mono ADPribose --- ADP-Ribosylation --- physiology --- ADP Ribose Transferases. --- Cellular signal transduction. --- G proteins --- Molecular microbiology --- Physiological effect. --- Genetics --- Congresses. --- Cell Signaling --- Bacterial Toxin --- Toxin, Bacterial --- Fungi. --- Antibiotics --- Lactones --- Streptomyces --- Yeasts