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Abies alba --- Arundo donax --- Corsica --- Fagus sylvatica --- Gomphocarpus fruticosus --- Helleborus trifolius --- Nerium oleander --- Pinus nigra

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This second volume of the Special Issue includes 13 contributions, from across the world, with very interesting research topics such as: Energy Intensity Reduction; Large-Scale Non-Residential Buildings; Dynamic Control of HVAC with Heat Pumps; Reed Potential as a Regenerative Building Material and Their Performance Characterisation; Experimental Evaluation of Energy-Efficiency in a Holistically Designed Building; A Case Study of Design and Energy Performance Analysis of a Hotel Building in a Hot and Dry Climate; Internal Convective and Radiative Heat Transfer Coefficients for a Vertical Wall in a Residential Building; Passive Façade Performance Evaluation with Shape Memory Alloy (SMA) during the Architectural Design Process; Thermal Performance Improvement of Double-Pane Lightweight Steel Framed (LSF) Walls Using Thermal Break Strips (TBS) and Reflective Foils; Energy Performance of Buildings with Thermochromic Windows in Mediterranean Climates; Energy Performance and Benchmarking for University Classrooms in Hot and Humid Climates; Effect of HVAC’s Management on Indoor Thermo-Hygrometric Comfort and Energy Balance: In Situ Assessments on a Real nearly Zero Energy Building (nZEB); Stochastic-Based Optimisation Approach towards the Integration of Photovoltaic Panels in Multi-Residential Social Housing; Effect of Climate Change and Occupant Behaviour on the Environmental Impact of the Heating/Cooling Systems of a Real Apartment: A Parametric Study through Life Cycle Assessment; Road Thermal Collector for Building Heating in South Europe (Italy): Numerical Modeling and Design of an Experimental Set-Up.

Technology: general issues --- Chemical engineering --- energy efficiency improvement --- HVAC operation --- dynamic optimization --- sustainability --- reed (Arundo donax) --- material characterisation --- natural materials --- vernacular architecture --- holistic --- energy management system --- sustainable --- building performance --- thermal performance --- indoor comfort --- energy performance --- design parameters --- energy simulation --- building envelope --- convection --- radiation --- heat transfer coefficient --- correlation --- climate-adaptive building shells --- sustainable design --- energy efficiency --- shape-memory alloy --- climate change --- experimental assessment --- double-pane --- lightweight steel frame (LSF) --- partition walls --- aerogel thermal break strips --- aluminium reflective foils --- thermochromic coatings --- solar transmittance --- solar reflectance --- Energy Use Intensity --- higher education buildings --- energy consumption --- benchmarking --- hot and humid climates --- EnergyPlus --- nZEB --- HVAC management --- monitoring campaign --- load matching --- computational fluid dynamics --- social housing --- energy demand --- energy refurbishment --- internal gains --- occupation rate --- life cycle cost --- life cycle assessment (LCA) --- ReCiPe indicator --- global warming potential (GWP) indicator --- environmental impact --- heating and cooling systems --- occupant behaviour --- road thermal collector --- borehole thermal storage --- alternative energy system

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The understanding of the physical and genetic structure of plant genomes and the determination of phenotypes via interactions of coding and non-coding regions with the environment has quickly become the key to the future of plant breeding and agriculture. In Transgenic Wheat, Barley and Oats: Production and Characterization Protocols, expert investigators contribute the latest protocols for the transformation, regeneration and selection of these three species, using both biolistics and Agrobacterium tumefaciens, in order to fill the need for techniques and resources in this vital area of research. Covering such topics as in vitro Agrobacterium co-cultivation, the manipulation of gene expression, and GM risk assessment, this versatile collection focuses on the study of temperate small grain cereals while many of its readily reproducible procedures could be easily adapted to accommodate other cereals and plants. The in-depth chapters adhere to the highly successful Methods in Molecular Biology™ series format, featuring brief introductions to the subject, lists of the necessary materials and reagents, step-by-step laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Transgenic Wheat, Barley and Oats: Production and Characterization Protocols is an ideal guide for scientists pursuing this powerful, forward-thinking genetic strategy.

Transgenic plants --- Wheat --- Barley --- Oats --- Avena sativa --- Hordeum --- Laboratory Manuals --- Triticum --- Plants, Genetically Modified --- Genetic Engineering --- Methods --- Genetics --- Cereals --- Animal Feed --- Publication Formats --- Biology --- Poaceae --- Investigative Techniques --- Organisms, Genetically Modified --- Plants --- Genetic Techniques --- Publication Characteristics --- Food --- Biological Science Disciplines --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Organism Forms --- Crops, Agricultural --- Angiosperms --- Eukaryota --- Embryophyta --- Food and Beverages --- Organisms --- Natural Science Disciplines --- Disciplines and Occupations --- Technology, Industry, Agriculture --- Streptophyta --- Viridiplantae --- Plant Sciences --- Agriculture --- Earth & Environmental Sciences --- Natural Sciences --- Physical Sciences --- Discipline, Natural Science --- Disciplines, Natural Science --- Natural Science --- Natural Science Discipline --- Physical Science --- Science, Natural --- Science, Physical --- Sciences, Natural --- Sciences, Physical --- Beverages and Food --- Land Plants --- Land Plant --- Plant, Land --- Plants, Land --- Eucarya --- Eukarya --- Eukaryotes --- Eukaryotas --- Eukaryote --- Angiospermae --- Liliopsida --- Magnoliopsida --- Angiosperm --- Liliopsidas --- Magnoliopsidas --- Agricultural Crops --- Agricultural Crop --- Biologic Sciences --- Biological Science --- Science, Biological --- Sciences, Biological --- Biological Sciences --- Life Sciences --- Biologic Science --- Biological Science Discipline --- Discipline, Biological Science --- Disciplines, Biological Science --- Life Science --- Science Discipline, Biological --- Science Disciplines, Biological --- Science, Biologic --- Science, Life --- Sciences, Biologic --- Sciences, Life --- Nutrients --- Foods --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Plant --- GMO Organisms --- Genetically Engineered Organisms --- Genetically Modified Organisms --- Organisms, Transgenic --- Transgenic Organisms --- Engineered Organism, Genetically --- Engineered Organisms, Genetically --- GMO Organism --- Genetically Engineered Organism --- Genetically Modified Organism --- Modified Organism, Genetically --- Modified Organisms, Genetically --- Organism, GMO --- Organism, Genetically Engineered --- Organism, Genetically Modified --- Organism, Transgenic --- Organisms, GMO --- Organisms, Genetically Engineered --- Transgenic Organism --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Grass --- Imperata cylindrica --- Alopecurus --- Arundo --- Gramineae --- Grasses --- Imperata --- Fodder --- Animal Feeds --- Feed, Animal --- Feeds, Animal --- Fodders --- Cereal Grain --- Cereal --- Cereal Grains --- Edible Grains --- Grain, Cereal --- Grain, Edible --- Grains, Cereal --- Grains, Edible --- Methodological Studies --- Methodological Study --- Procedures --- Studies, Methodological --- Study, Methodological --- Method --- Procedure --- Engineering, Genetic --- Intervention, Genetic --- Genetic Intervention --- Genetic Interventions --- Interventions, Genetic --- GMO Plants --- Genetically Engineered Plants --- Genetically Modified Plants --- Plants, Transgenic --- Transgenic Plants --- Engineered Plant, Genetically --- Engineered Plants, Genetically --- GMO Plant --- Genetically Engineered Plant --- Genetically Modified Plant --- Modified Plant, Genetically --- Modified Plants, Genetically --- Plant, GMO --- Plant, Genetically Engineered --- Plant, Genetically Modified --- Plant, Transgenic --- Plants, GMO --- Plants, Genetically Engineered --- Transgenic Plant --- Durum Wheat --- Triticum aestivum --- Triticum durum --- Triticum spelta --- Triticum turgidum --- Triticum turgidum subsp. durum --- Triticum vulgare --- Durum Wheats --- Wheat, Durum --- Hordeum vulgare --- Avena --- Common oat --- Oat, Common --- GE crops (Genetically engineered crops) --- GE plants (Genetically engineered plants) --- Genetically engineered crops --- Genetically engineered plants --- Genetically modified crops --- Genetically modified plants --- GM crops (Genetically modified crops) --- GM plants (Genetically modified plants) --- Novel crops --- Transgenic crops --- methods --- Green Plants --- Green Plant --- Plant, Green --- Plants, Green --- Cultivated Oat --- Avenas --- Cultivated Oats --- Oat --- Oat, Cultivated --- Oats, Cultivated --- Chemistry. --- Biotechnology. --- Genetic engineering. --- Transgenic organisms. --- Genetic Engineering. --- Food Science. --- Transgenics. --- Food science. --- Science --- Genetically engineered organisms --- Genetically modified organisms --- GEOs (Genetically engineered organisms) --- GMOs (Genetically modified organisms) --- Genetic engineering --- Designed genetic change --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Chemical engineering --- Food technology