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This detailed book presents a technical overview and practical methodology of a variety of antibody array formats and technologies. As advantages and disadvantages of antibody array types are explored, the volume also delves into practical applications of antibody arrays pertaining to investigations of specific research topics and biological processes as well as guidance on the methods of processing, analysis, and storage of array data. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Antibody Arrays: Methods and Protocols aims to empower the reader with the information required to select the most appropriate array for their research application, with the technical knowledge to use and process the array, and with the knowledge to perform analysis that realizes the maximum benefit from the data generated.

Immunoglobulins. --- Protein microarrays. --- Antibodies. --- Protein Array Analysis. --- Microarray Analysis. --- Microarrays, Protein --- Peptide biochips --- Peptide microarrays --- Protein biochips --- Biochips --- Immobilized proteins --- Antibodies --- Immune globulins --- Immune serum globulin --- Blood proteins --- Globulins --- Plasma cells --- Antibody diversity --- Antigens --- Bacterial immunoglobulin-binding proteins --- Microarray Analytical Devices --- Microarray Microchips --- Nanoarray Analytical Devices --- Analysis, Microarray --- Analytical Device, Microarray --- Analytical Device, Nanoarray --- Analytical Devices, Microarray --- Analytical Devices, Nanoarray --- Device, Microarray Analytical --- Device, Nanoarray Analytical --- Devices, Microarray Analytical --- Devices, Nanoarray Analytical --- Microarray Analytical Device --- Microarray Microchip --- Microchip, Microarray --- Microchips, Microarray --- Nanoarray Analytical Device --- Protein Array Assay --- Protein Arrays --- Protein Biochips --- Protein Microarray Analysis --- Protein Microarray Assay --- Protein Profiling Microarrays --- ProteinChip --- Protein Chips --- Protein Microarrays --- Protein Microchips --- Protein Profiling Chips --- Analyses, Protein Array --- Analyses, Protein Microarray --- Analysis, Protein Array --- Analysis, Protein Microarray --- Array Analyses, Protein --- Array Analysis, Protein --- Array Assay, Protein --- Array Assays, Protein --- Array, Protein --- Arrays, Protein --- Assay, Protein Array --- Assay, Protein Microarray --- Assays, Protein Array --- Assays, Protein Microarray --- Biochip, Protein --- Biochips, Protein --- Chip, Protein --- Chip, Protein Profiling --- Chips, Protein --- Chips, Protein Profiling --- Microarray Analyses, Protein --- Microarray Analysis, Protein --- Microarray Assay, Protein --- Microarray Assays, Protein --- Microarray, Protein --- Microarray, Protein Profiling --- Microarrays, Protein Profiling --- Microchip, Protein --- Microchips, Protein --- Profiling Chip, Protein --- Profiling Chips, Protein --- Profiling Microarray, Protein --- Profiling Microarrays, Protein --- Protein Array --- Protein Array Analyses --- Protein Array Assays --- Protein Biochip --- Protein Chip --- Protein Microarray --- Protein Microarray Analyses --- Protein Microarray Assays --- Protein Microchip --- Protein Profiling Chip --- Protein Profiling Microarray --- ProteinChips --- Protein Interaction Mapping --- Biology—Technique. --- Chemistry. --- Biological Techniques. --- Physical sciences

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 Recent improvements in the efficiency, quality, and cost of genome-wide sequencing have prompted biologists and biomedical researchers to move away from microarray-based technology to ultra high-throughput, massively parallel genomic sequencing (Next Generation Sequencing, NGS) technology. In Next Generation Microarray Bioinformatics: Methods and Protocols, expert researchers in the field provide techniques to bring together current computational and statistical methods to analyze and interpreting both microarray and NGS data. These methods and techniques include resources for microarray bioinformatics, microarray data analysis, microarray bioinformatics in systems biology, next generation sequencing data analysis, and emerging applications of microarray and next generation sequencing. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.   Authoritative and practical, Next Generation Microarray Bioinformatics: Methods and Protocols seeks to aid scientists in the further study of this crucially important research into the human DNA.

Bioinformatics. --- DNA microarrays. --- Computational Biology. --- Microarray Analysis. --- DNA microarrays --- Bioinformatics --- Nucleic Acid Hybridization --- Sequence Analysis --- Molecular Probe Techniques --- Biology --- Microchip Analytical Procedures --- Genetic Techniques --- Investigative Techniques --- Chemistry Techniques, Analytical --- Biological Science Disciplines --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Natural Science Disciplines --- Disciplines and Occupations --- Microarray Analysis --- Oligonucleotide Array Sequence Analysis --- Computational Biology --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- Bio-Informatics --- Biology, Computational --- Computational Molecular Biology --- Molecular Biology, Computational --- Bio Informatics --- Bio-Informatic --- Bioinformatic --- Biologies, Computational Molecular --- Biology, Computational Molecular --- Computational Molecular Biologies --- Molecular Biologies, Computational --- DNA Arrays --- DNA Chips --- DNA Microchips --- Gene Chips --- Oligodeoxyribonucleotide Array Sequence Analysis --- Oligonucleotide Microarrays --- Sequence Analysis, Oligonucleotide Array --- cDNA Arrays --- DNA Microarrays --- Oligonucleotide Arrays --- cDNA Microarrays --- Array, DNA --- Array, Oligonucleotide --- Array, cDNA --- Arrays, DNA --- Arrays, Oligonucleotide --- Arrays, cDNA --- Chip, DNA --- Chip, Gene --- Chips, DNA --- Chips, Gene --- DNA Array --- DNA Chip --- DNA Microarray --- DNA Microchip --- Gene Chip --- Microarray, DNA --- Microarray, Oligonucleotide --- Microarray, cDNA --- Microarrays, DNA --- Microarrays, Oligonucleotide --- Microarrays, cDNA --- Microchip, DNA --- Microchips, DNA --- Oligonucleotide Array --- Oligonucleotide Microarray --- cDNA Array --- cDNA Microarray --- Microarray Analytical Devices --- Microarray Microchips --- Nanoarray Analytical Devices --- Analysis, Microarray --- Analytical Device, Microarray --- Analytical Device, Nanoarray --- Analytical Devices, Microarray --- Analytical Devices, Nanoarray --- Device, Microarray Analytical --- Device, Nanoarray Analytical --- Devices, Microarray Analytical --- Devices, Nanoarray Analytical --- Microarray Analytical Device --- Microarray Microchip --- Microchip, Microarray --- Microchips, Microarray --- Nanoarray Analytical Device --- Natural Sciences --- Physical Sciences --- Discipline, Natural Science --- Disciplines, Natural Science --- Natural Science --- Natural Science Discipline --- Physical Science --- Science, Natural --- Science, Physical --- Sciences, Natural --- Sciences, Physical --- Biologic Sciences --- Biological Science --- Science, Biological --- Sciences, Biological --- Biological Sciences --- Life Sciences --- Biologic Science --- Biological Science Discipline --- Discipline, Biological Science --- Disciplines, Biological Science --- Life Science --- Science Discipline, Biological --- Science Disciplines, Biological --- Science, Biologic --- Science, Life --- Sciences, Biologic --- Sciences, Life --- Analytical Chemistry Methods --- Analytical Chemistry Techniques --- Analytical Chemistry Method --- Analytical Chemistry Technique --- Chemistry Method, Analytical --- Chemistry Methods, Analytical --- Chemistry Technique, Analytical --- Method, Analytical Chemistry --- Methods, Analytical Chemistry --- Technique, Analytical Chemistry --- Techniques, Analytical Chemistry --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Genetics --- Microchip Analysis --- Analyses, Microchip --- Analysis, Microchip --- Analytical Procedure, Microchip --- Analytical Procedures, Microchip --- Microchip Analytical Procedure --- Procedure, Microchip Analytical --- Procedures, Microchip Analytical --- Molecular Probe Technic --- Molecular Probe Technics --- Molecular Probe Technique --- Technic, Molecular Probe --- Technics, Molecular Probe --- Technique, Molecular Probe --- Techniques, Molecular Probe --- Probe Technic, Molecular --- Probe Technics, Molecular --- Probe Technique, Molecular --- Probe Techniques, Molecular --- DNA biochips --- Analysis, Sequence --- Determination, Sequence --- Determinations, Sequence --- Sequence Determinations --- Sequence Determination --- Analyses, Sequence --- Sequence Analyses --- Genomic Hybridization --- Acid Hybridization, Nucleic --- Acid Hybridizations, Nucleic --- Genomic Hybridizations --- Hybridization, Genomic --- Hybridization, Nucleic Acid --- Hybridizations, Genomic --- Hybridizations, Nucleic Acid --- Nucleic Acid Hybridizations --- Bio-informatics --- Biological informatics --- methods --- Life sciences. --- Life Sciences. --- Information science --- Computational biology --- Systems biology --- Biosciences --- Science --- Data processing

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Presenting an area of research that intersects with and integrates diverse disciplines, including genomics, epigenetics, proteomics, and cellular biology, among others, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols collects contributions from expert researchers in order to provide practical guidelines to this complex study. Compiled in order to provide researchers with up-to-date methodological information pertaining to the utilization of genomic, transcriptomic, and proteomic data in diagnosis, prognosis, and tailored therapy, the ultimate aim of this volume is to decipher diseases at a molecular level. Divided into multiple convenient chapters, this detailed book covers various techniques to construct and utilize tissue arrays, it also provides detailed protocols in immunohistochemistry, immunofluorescence, fluorescent and chromogenic in situ hybridization, and ultimately introduces protocols for FFPET microdissection and nucleic acids extraction for their utilization in advanced techniques such as microarray CGH, DNA methylation and pyrosequencing. The volume also discusses FFPET research from an ethical standpoint and concludes with a chapter on novel tissue fixative. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and accessible, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols serves as a practical guide for scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this vital field of research.

Human histology. Human cytology --- Chemical laboratory practice --- Tissues --- Tissue culture --- Diagnostic immunohistochemistry --- Tissue Embedding --- Genetic Techniques --- Microarray Analysis --- Culture Techniques --- Publication Formats --- Investigative Techniques --- Clinical Laboratory Techniques --- Histocytological Preparation Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Publication Characteristics --- Microchip Analytical Procedures --- Cytological Techniques --- Chemistry Techniques, Analytical --- Histological Techniques --- Gene Expression Profiling --- Laboratory Manuals --- Paraffin Embedding --- Tissue Array Analysis --- Methods --- Tissue Culture Techniques --- Human Anatomy & Physiology --- Biology --- Health & Biological Sciences --- Physiology --- Biophysics --- Analysis --- Embedding, Paraffin --- Gene Expression Monitoring --- Transcriptome Analysis --- Gene Expression Pattern Analysis --- Transcript Expression Analysis --- Transcriptome Profiling --- mRNA Differential Display --- Analyses, Transcript Expression --- Analyses, Transcriptome --- Analysis, Transcript Expression --- Analysis, Transcriptome --- Differential Display, mRNA --- Differential Displays, mRNA --- Display, mRNA Differential --- Displays, mRNA Differential --- Gene Expression Monitorings --- Gene Expression Profilings --- Monitoring, Gene Expression --- Monitorings, Gene Expression --- Profiling, Gene Expression --- Profiling, Transcriptome --- Profilings, Gene Expression --- Profilings, Transcriptome --- Transcript Expression Analyses --- Transcriptome Analyses --- Transcriptome Profilings --- mRNA Differential Displays --- Histologic Technic --- Histologic Technics --- Histologic Technique --- Histologic Techniques --- Histological Technics --- Technic, Histologic --- Technics, Histologic --- Technique, Histologic --- Techniques, Histologic --- Histological Technic --- Histological Technique --- Technic, Histological --- Technics, Histological --- Technique, Histological --- Techniques, Histological --- Histology --- Analytical Chemistry Methods --- Analytical Chemistry Techniques --- Analytical Chemistry Method --- Analytical Chemistry Technique --- Chemistry Method, Analytical --- Chemistry Methods, Analytical --- Chemistry Technique, Analytical --- Method, Analytical Chemistry --- Methods, Analytical Chemistry --- Technique, Analytical Chemistry --- Techniques, Analytical Chemistry --- Cytologic Technics --- Cytological Technic --- Cytological Technics --- Cytological Technique --- Technic, Cytological --- Technics, Cytological --- Technique, Cytological --- Techniques, Cytological --- Cytologic Technic --- Technic, Cytologic --- Technics, Cytologic --- Cell Biology --- Microchip Analysis --- Analyses, Microchip --- Analysis, Microchip --- Analytical Procedure, Microchip --- Analytical Procedures, Microchip --- Microchip Analytical Procedure --- Procedure, Microchip Analytical --- Procedures, Microchip Analytical --- Cytohistologic Preparation Technics --- Cytohistologic Preparation Technique --- Cytohistologic Preparation Techniques --- Cytohistological Preparation Technic --- Cytohistological Preparation Technics --- Cytohistological Preparation Technique --- Histocytologic Preparation Technic --- Histocytologic Preparation Technics --- Histocytologic Preparation Technique --- Histocytologic Preparation Techniques --- Histocytological Preparation Technic --- Histocytological Preparation Technics --- Histocytological Preparation Technique --- Preparation Technic, Cytohistologic --- Preparation Technic, Cytohistological --- Preparation Technic, Histocytologic --- Preparation Technic, Histocytological --- Preparation Technics, Cytohistologic --- Preparation Technics, Cytohistological --- Preparation Technics, Histocytologic --- Preparation Technics, Histocytological --- Preparation Technique, Cytohistologic --- Preparation Technique, Cytohistological --- Preparation Technique, Histocytologic --- Preparation Technique, Histocytological --- Preparation Techniques, Cytohistologic --- Preparation Techniques, Cytohistological --- Preparation Techniques, Histocytologic --- Preparation Techniques, Histocytological --- Technic, Cytohistologic Preparation --- Technic, Cytohistological Preparation --- Technic, Histocytologic Preparation --- Technic, Histocytological Preparation --- Technics, Cytohistologic Preparation --- Technics, Cytohistological Preparation --- Technics, Histocytologic Preparation --- Technics, Histocytological Preparation --- Technique, Cytohistologic Preparation --- Technique, Cytohistological Preparation --- Technique, Histocytologic Preparation --- Technique, Histocytological Preparation --- Techniques, Cytohistologic Preparation --- Techniques, Cytohistological Preparation --- Techniques, Histocytologic Preparation --- Techniques, Histocytological Preparation --- Cytohistological Preparation Techniques --- Cytohistologic Preparation Technic --- Diagnoses and Laboratory Examinations --- Diagnosis, Laboratory --- Laboratory Diagnosis --- Laboratory Examinations and Diagnoses --- Laboratory Techniques, Clinical --- Clinical Laboratory Technique --- Diagnoses, Laboratory --- Laboratory Diagnoses --- Laboratory Technique, Clinical --- Technique, Clinical Laboratory --- Techniques, Clinical Laboratory --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Culture Technique --- Technique, Culture --- Techniques, Culture --- Microarray Analytical Devices --- Microarray Microchips --- Nanoarray Analytical Devices --- Analysis, Microarray --- Analytical Device, Microarray --- Analytical Device, Nanoarray --- Analytical Devices, Microarray --- Analytical Devices, Nanoarray --- Device, Microarray Analytical --- Device, Nanoarray Analytical --- Devices, Microarray Analytical --- Devices, Nanoarray Analytical --- Microarray Analytical Device --- Microarray Microchip --- Microchip, Microarray --- Microchips, Microarray --- Nanoarray Analytical Device --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Genetics --- Diagnostic immunohistology --- Culture Technique, Tissue --- Culture Techniques, Tissue --- Tissue Culture Technique --- Methodological Studies --- Methodological Study --- Procedures --- Studies, Methodological --- Study, Methodological --- Method --- Procedure --- methods --- Cultures and culture media --- Cell Array Analysis --- Cell Microarray Analysis --- Tissue Microarray Analysis --- Analyses, Cell Array --- Analyses, Cell Microarray --- Analyses, Tissue Array --- Analyses, Tissue Microarray --- Analysis, Cell Array --- Analysis, Cell Microarray --- Analysis, Tissue Array --- Analysis, Tissue Microarray --- Array Analyses, Cell --- Array Analyses, Tissue --- Array Analysis, Cell --- Array Analysis, Tissue --- Cell Array Analyses --- Cell Microarray Analyses --- Microarray Analyses, Cell --- Microarray Analyses, Tissue --- Microarray Analysis, Cell --- Microarray Analysis, Tissue --- Tissue Array Analyses --- Tissue Microarray Analyses --- Embedding, Tissue --- Medicine. --- Pathology. --- Molecular Medicine. --- Disease (Pathology) --- Medical sciences --- Diseases --- Medicine --- Medicine, Preventive --- Health Workforce

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Gene Expression Profiling --- Nervous System Diseases --- Oligonucleotide Array Sequence Analysis --- Nervous System Physiological Phenomena --- Microarray Analysis --- Sequence Analysis --- Molecular Probe Techniques --- Genetic Techniques --- Nucleic Acid Hybridization --- Musculoskeletal and Neural Physiological Phenomena --- Diseases --- Microchip Analytical Procedures --- Investigative Techniques --- Phenomena and Processes --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Chemistry Techniques, Analytical --- Analytical Chemistry Methods --- Analytical Chemistry Techniques --- Analytical Chemistry Method --- Analytical Chemistry Technique --- Chemistry Method, Analytical --- Chemistry Methods, Analytical --- Chemistry Technique, Analytical --- Method, Analytical Chemistry --- Methods, Analytical Chemistry --- Technique, Analytical Chemistry --- Techniques, Analytical Chemistry --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Microchip Analysis --- Analyses, Microchip --- Analysis, Microchip --- Analytical Procedure, Microchip --- Analytical Procedures, Microchip --- Microchip Analytical Procedure --- Procedure, Microchip Analytical --- Procedures, Microchip Analytical --- Micromanipulation --- Nanotechnology --- Lab-On-A-Chip Devices --- Musculoskeletal and Neural Physiological Concepts --- Musculoskeletal and Neural Physiological Phenomenon --- Musculoskeletal and Neural Physiology --- Genomic Hybridization --- Acid Hybridization, Nucleic --- Acid Hybridizations, Nucleic --- Genomic Hybridizations --- Hybridization, Genomic --- Hybridization, Nucleic Acid --- Hybridizations, Genomic --- Hybridizations, Nucleic Acid --- Nucleic Acid Hybridizations --- DNA --- Nucleic Acid Probes --- Oligonucleotide Probes --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Genetics --- Genetic Counseling --- Genetic Phenomena --- Molecular Probe Technic --- Molecular Probe Technics --- Molecular Probe Technique --- Technic, Molecular Probe --- Technics, Molecular Probe --- Technique, Molecular Probe --- Techniques, Molecular Probe --- Probe Technic, Molecular --- Probe Technics, Molecular --- Probe Technique, Molecular --- Probe Techniques, Molecular --- Analysis, Sequence --- Determination, Sequence --- Determinations, Sequence --- Sequence Determinations --- Sequence Determination --- Analyses, Sequence --- Sequence Analyses --- Microarray Analytical Devices --- Microarray Microchips --- Nanoarray Analytical Devices --- Analysis, Microarray --- Analytical Device, Microarray --- Analytical Device, Nanoarray --- Analytical Devices, Microarray --- Analytical Devices, Nanoarray --- Device, Microarray Analytical --- Device, Nanoarray Analytical --- Devices, Microarray Analytical --- Devices, Nanoarray Analytical --- Microarray Analytical Device --- Microarray Microchip --- Microchip, Microarray --- Microchips, Microarray --- Nanoarray Analytical Device --- Nervous System Physiological Concepts --- Nervous System Physiological Phenomenon --- Nervous System Physiological Process --- Physiology, Nervous System --- Nervous System Physiologic Processes --- Nervous System Physiological Processes --- Nervous System Physiology --- System Physiology, Nervous --- Nervous System --- DNA Arrays --- DNA Chips --- DNA Microchips --- Gene Chips --- Oligodeoxyribonucleotide Array Sequence Analysis --- Oligonucleotide Microarrays --- Sequence Analysis, Oligonucleotide Array --- cDNA Arrays --- DNA Microarrays --- Oligonucleotide Arrays --- cDNA Microarrays --- Array, DNA --- Array, Oligonucleotide --- Array, cDNA --- Arrays, DNA --- Arrays, Oligonucleotide --- Arrays, cDNA --- Chip, DNA --- Chip, Gene --- Chips, DNA --- Chips, Gene --- DNA Array --- DNA Chip --- DNA Microarray --- DNA Microchip --- Gene Chip --- Microarray, DNA --- Microarray, Oligonucleotide --- Microarray, cDNA --- Microarrays, DNA --- Microarrays, Oligonucleotide --- Microarrays, cDNA --- Microchip, DNA --- Microchips, DNA --- Oligonucleotide Array --- Oligonucleotide Microarray --- cDNA Array --- cDNA Microarray --- Combinatorial Chemistry Techniques --- Nervous System Disorders --- Neurological Disorders --- Neurologic Disorders --- Disease, Nervous System --- Diseases, Nervous System --- Disorder, Nervous System --- Disorder, Neurologic --- Disorder, Neurological --- Disorders, Nervous System --- Disorders, Neurologic --- Disorders, Neurological --- Nervous System Disease --- Nervous System Disorder --- Neurologic Disorder --- Neurological Disorder --- Neurology --- Gene Expression Monitoring --- Transcriptome Analysis --- Gene Expression Pattern Analysis --- Transcript Expression Analysis --- Transcriptome Profiling --- mRNA Differential Display --- Analyses, Transcript Expression --- Analyses, Transcriptome --- Analysis, Transcript Expression --- Analysis, Transcriptome --- Differential Display, mRNA --- Differential Displays, mRNA --- Display, mRNA Differential --- Displays, mRNA Differential --- Gene Expression Monitorings --- Gene Expression Profilings --- Monitoring, Gene Expression --- Monitorings, Gene Expression --- Profiling, Gene Expression --- Profiling, Transcriptome --- Profilings, Gene Expression --- Profilings, Transcriptome --- Transcript Expression Analyses --- Transcriptome Analyses --- Transcriptome Profilings --- mRNA Differential Displays --- Gene Expression --- Transcriptome --- Subtractive Hybridization Techniques --- methods --- physiology --- Gene Expression Microarray Analysis --- Gene Expression Analysis --- Transcriptomics --- Analyses, Gene Expression --- Analysis, Gene Expression --- Expression Analyses, Gene --- Expression Analysis, Gene --- Gene Expression Analyses