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Etude de la fructose-2,6-bisphosphatase de cellules d'hépatome de rat
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Nous avons étudié le mécanisme d’action, les propriétés cinétiques et physiques de la FBPase-2 des cellules HTC. Il fallait la purifier partiellement afin de la débarrasser des contaminants gênants et notamment de la PFK-2 pour obtenir une enzyme monofonctionnelle.
Dans un premier temps, la méthode de dosage de l’activité enzymatique de la FBPase-2 a été rendue la plus sensible possible pour permettre le dosage de l’activité enzymatique de la FBPase-2 d’HTC dans les premières étapes de la purification.
Dans cette optique, deux méthodes de dosage ont été envisagées : le dosage par méthode fluorimétrique et le dosage par méthode radioactive.
Le dosage estimé suffisamment sensible, la purification partielle a pu être entamée d’après le schéma suivant :
- centrifugation de l’homogénat cellulaire
- précipitation au polyéthylène glycol (PEG)
- chromatographie sur l’échangeur d’ions Mono Q. L’enzyme est éluée avec un gradient de chlorure de potassium.


Book
Biochemical Adaptation
Authors: ---
ISBN: 0691612633 1400855411 0691083444 0691083436 9781400855414 9780691083438 9780691083445 Year: 2014 Publisher: Princeton, NJ

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This book discusses biochemical adaptation to environments from freezing polar oceans to boiling hot springs, and under hydrostatic pressures up to 1,000 times that at sea level.Originally published in 1984.The Princeton Legacy Library uses the latest print-on-demand technology to again make available previously out-of-print books from the distinguished backlist of Princeton University Press. These editions preserve the original texts of these important books while presenting them in durable paperback and hardcover editions. The goal of the Princeton Legacy Library is to vastly increase access to the rich scholarly heritage found in the thousands of books published by Princeton University Press since its founding in 1905.

Keywords

Adaptation (Physiology) --- Biochemistry. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Compensation (Physiology) --- Plasticity (Physiology) --- Composition --- Biology --- Chemistry --- Medical sciences --- Ecophysiology --- Biochemistry --- 57.017.32 --- 575.826 --- 575.826 Adaptation --- Adaptation --- Biologische wetenschappen in het algemeen. Biologie--?.017.32 --- 6-bisphosphatase. --- ATPase. --- Acid–base homeostasis. --- Adenosine monophosphate. --- Alanine. --- Alcohol dehydrogenase. --- Amino acid. --- Aminooxyacetic acid. --- Anabolism. --- Anaerobic glycolysis. --- Antifreeze. --- Arginine. --- Basal rate. --- Beta oxidation. --- Bohr effect. --- Carbohydrate. --- Carnitine. --- Catabolism. --- Catalase. --- Catalysis. --- Cellular respiration. --- Cofactor (biochemistry). --- Competitive inhibition. --- Cooperativity. --- Deep sea. --- Dehydrogenase. --- Detergent. --- Dissociation constant. --- Enzyme Repression. --- Enzyme inhibitor. --- Enzyme. --- Facultative anaerobic organism. --- Fatty acid. --- Fermentation. --- Flavin adenine dinucleotide. --- Fructose 1. --- Futile cycle. --- Glucagon. --- Gluconeogenesis. --- Glucose-6-phosphate dehydrogenase. --- Glucose. --- Glyceraldehyde 3-phosphate dehydrogenase. --- Glycerol. --- Glycogen phosphorylase. --- Glycogen. --- Glycogenolysis. --- Glycolysis. --- Hemoglobin. --- Hibernation. --- High-energy phosphate. --- Hill equation (biochemistry). --- Histidine. --- Hofmeister series. --- Hormone-sensitive lipase. --- Insulin. --- Isozyme. --- Ketosis. --- Lactic acid. --- Lipid. --- Lipolysis. --- Lysine. --- Mammalian diving reflex. --- Metabolic intermediate. --- Metabolism. --- Michaelis–Menten kinetics. --- Mitochondrial matrix. --- Mitochondrion. --- Molecular mimicry. --- Muscle. --- Nicotinamide adenine dinucleotide. --- Obligate anaerobe. --- Obligate. --- Organism. --- Ornithine. --- Osmolyte. --- Oxidative deamination. --- Peroxidase. --- Phosphagen. --- Phosphofructokinase. --- Phospholipid. --- Phosphorylase kinase. --- Proline. --- Proofreading (biology). --- Protein turnover. --- Protein. --- Proteolysis. --- Pyruvate carboxylase. --- Pyruvic acid. --- Redox. --- Regulatory enzyme. --- Root effect. --- Substrate-level phosphorylation. --- Thermoregulation. --- Thermus aquaticus. --- Thermus thermophilus. --- Triglyceride. --- Tryptophan. --- Turnover number. --- Urea cycle. --- Urea.


Book
Plant Therapeutics
Authors: --- ---
ISBN: 3036559558 3036559566 Year: 2022 Publisher: Basel MDPI - Multidisciplinary Digital Publishing Institute

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This Special Issue provides recent advances in the use of plants for therapeutic purposes. This Special Issue collected the plants, including leaf, fruit, and others. This Special Issue's targets were crude plant extract and active principle purified from the plant. This Special Issue prompted researchers to provide each plant discovery. We are pleased to include in this Special Issue the screened Mexican plants and the halophytic plants growing in central Saudi Arabia, and so on. As a result, we have sparked scientists' interest in studying the plant for therapeutic purposes. This field necessitates network pharmacology analysis and machine-aided learning. Many disorders resistant to modern medication are looking for active principles isolated from the plants all over the world.

Keywords

Medicine --- Pharmacology --- safflower --- hydroxysafflor yellow A --- safflower yellow A --- flavonoid --- platelet aggregation --- vescalagin --- methylglyoxal --- inflammation --- antioxidant --- insulin secretion --- traditional medicine --- hypoglycemic plants --- Eryngium longifolium --- Alsophila firma --- glucose 6-phosphatase --- fructose 1,6-bisphosphatase --- Lycium shawii --- Anabasis articulata --- Rumex vesicarius --- Zilla spinosa --- anticancer --- antimicrobial --- biogenetic interrelationship --- flavonoid contents --- trace elements --- human tumor cell lines --- cytotoxic effect --- plant extracts --- Acalypha monostachya --- Lindera obtusiloba --- apolipoprotein E-deficient mice --- atherosclerosis --- endothelium --- black bean soybean sauce --- by-product --- functional supplements --- Moringa oleifera --- anti-aging --- fibrosis --- kidney disease --- Gan-Mai-Da-Zao --- depression --- brain-derived neurotrophic factor --- serotonin transporter --- unpredictable chronic mild stress --- Acalypha arvensis --- ellagitannin --- corilagin --- Staphylococcus aureus --- flavonoids --- antibacterial --- Alzheimer’s disease --- amyloid β --- immunomagnetic reduction --- Drosophila melanogaster --- water extract of fermented rice bran --- non-alcoholic fatty liver disease --- high-fat diet --- aged rat --- Salvia haematodes --- rapid solid–liquid dynamic extraction (RSLDE) --- extractor Naviglio® --- Soxhlet apparatus --- medium pressure liquid chromatography (MPLC) --- antioxidant potential --- Inga jinicuil --- phytochemical profile --- HPLC-PDA --- GC-MS --- anti-inflammatory --- fast dissolving films --- yeast β-glucan --- bilberry juice --- diabetes --- herbal medicine --- bioactive derivative --- triple negative breast cancer --- Wnt/β-catenin --- anti-cancer --- n/a --- Alzheimer's disease --- rapid solid-liquid dynamic extraction (RSLDE)

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