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mRNA processing and metabolism : methods and protocols
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ISBN: 1588292258 1592597505 128036047X 9786610360475 Year: 2004 Volume: 257 Publisher: Totowa, N.J. : Humana Press,

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Abstract

mRNA processing is a key step in gene expression that effects all the proteins within the cell. In mRNA Processing and Metabolism: Methods and Protocols, world-renowned researchers bring together the latest techniques spanning the breadth of mRNA processing and metabolism. Drawing on recent advances in microscopy, whole genome sequencing, microarrays, mass spectrometry, fluorescent detection methodologies, and RNA interference, the authors offer readily reproducible methods for the cotranscriptional processing events that occur while the mRNA is engaged with elongating RNA polymerase II, with splicing and its biochemical analysis and with alternative splicing. Additional methods cover mRNA export, the recovery and analysis of mRNP complexes, cytoplasmic translation, mRNA degradation in vivo and in vitro, and the controversial concept of nuclear translation. A variety of model organisms are used, including yeast, Drosophila, Xenopus, mice, plants, and cultured mammalian cells. Each proven protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Timely and authoritative, mRNA Processing and Metabolism: Methods and Protocols provides a powerful collection of novel techniques that are not only effective, but also immediately applicable to current problems in many areas of biological research.


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Gene transfer and expression : a laboratory manual
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ISBN: 033353543X 0935859896 9780935859898 9780333535431 Year: 1990 Publisher: New York (N.Y.): Stockton press,

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Keywords

Biological techniques --- Molecular biology --- Genetic transformation --- Gene expression --- Gene Expression Regulation --- Transfection --- Laboratory manuals --- Regulation --- Gene Expression Regulation. --- Transfection. --- 575.117 --- 577.216 --- 57.088.6 --- -Genetic transformation --- -#WSCH:WBIO --- Gene transfer --- Transformation (Genetics) --- Genetic recombination --- Microbial genetics --- Nucleic acids --- Genes --- Genetic regulation --- Transfections --- Transformation, Bacterial --- Transformation, Genetic --- Expression Regulation, Gene --- Regulation, Gene Action --- Regulation, Gene Expression --- Gene Action Regulation --- Regulation of Gene Expression --- RNAi Therapeutics --- Gene Regulatory Networks --- Expressivity and penetrance of genes --- Transfer of inheritance information. Transport of messenger RNA in the cell --- Methods and techniques for studying metabolism and biotransformation.Radioisotopes methods and techniques. --- -Laboratory manuals --- Expression --- Laboratory manuals. --- laboratory manuals. --- 57.088.6 Methods and techniques for studying metabolism and biotransformation.Radioisotopes methods and techniques. --- 577.216 Transfer of inheritance information. Transport of messenger RNA in the cell --- 575.117 Expressivity and penetrance of genes --- laboratory manuals --- #WSCH:WBIO --- Regulation&delete& --- Methods and techniques for studying metabolism and biotransformation.Radioisotopes methods and techniques --- Genetic transformation - Laboratory manuals --- Gene expression - Regulation - Laboratory manuals --- Gene Expression Regulation - laboratory manuals --- Transfection - laboratory manuals --- GENE EXPRESSION REGULATION --- TRANSFECTION --- LABORATORY MANUALS

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