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DNA sequencing from experimental methods to bioinformatics
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ISBN: 1859960618 0387915095 9780387915098 9781859960615 Year: 1997 Publisher: Oxford New York Bios Scientific Publishers Springer

Principles of proteomics
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ISBN: 1859962734 Year: 2004 Publisher: New York (N.Y.) : BIOS scientific,

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The Advanced Text series is designed for students taking advanced-level courses, including final year undergraduates and taught MSc. Marine microbiology is a rapidly expanding subject. New tools and an increased interest in ecological factors have caused an upsurge of interest in this field of study. This book aims to convey the fascinating discoveries and great importance of this fast-moving discipline to the student. Proteomics is a major new area of scientific research driven by the growing information gap between genes and their functions. Large-scale DNA sequencing and annotation projects identify thousands of new genes on a daily basis but the functions of these genes remain largely unknown. Proteins are the actual functional molecules of the cell, and technical advances in the high-throughput analysis of proteins now allow these molecules to be studied directly. Principles of Proteomics provides a comprehensive introduction to this relatively new field, for the first time bringing together all the disparate themes of proteomics. The book covers the development of large-scale technologies for protein separation, isolation, detection and quantitation. It describes in detail the role of mass spectrometry in high-throughput protein annotation. It also covers the emerging fields of structural proteomics, interaction proteomics and the recent development of chip-based analysis techniques. The final chapter provides some examples of how proteomics is being applied in the fields of medicine, biotechnology and agriculture.

Computer analysis of sequence data
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ISBN: 0896032469 0896032760 159259512X 1592595111 Year: 1994 Volume: 24, 25 Publisher: Totowa, N.J. : Humana Press,

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Abstract

DNA sequencing has become increasingly efficient over the years, resulting in an enormous increase in the amount of data gen- ated. In recent years, the focus of sequencing has shifted, from being the endpoint of a project, to being a starting point. This is especially true for such major initiatives as the human genome project, where vast tracts of DNA of unknown function are sequenced. This sheer volume of available data makes advanced computer methods ess- tial to analysis, and a familiarity with computers and sequence ana- sis software a vital requirement for the researcher involved with DNA sequencing. Even for nonsequencers, a familiarity with sequence analysis software can be important. For instance, gene sequences already present in the databases can be extremely useful in the design of cloning and genetic manipulation experiments. This two-part work on Analysis of Data is designed to be a practical aid to the researcher who uses computers for the acquisition, storage, or analysis of nucleic acid (and/or p- tein) sequences. Each chapter is written such that a competent sci- tist with basic computer literacy can carry out the procedure successfully at the first attempt by simply following the detailed pr- tical instructions that have been described by the author. A Notes section, which is included at the end of each chapter, provides advice on overcoming the common problems and pitfalls sometimes enco- tered by users of the sequence analysis software. Software packages for both the mainframe and personal computers are described.

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