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Episomes --- Plasmids --- Congresses --- -Plasmids --- -Paragenes --- Cytoplasmic inheritance --- Genetic vectors --- Mobile genetic elements --- Extrachromosomal DNA --- Bacterial genetics --- Insertion elements, DNA --- Molecular genetics --- -Congresses --- Congresses. --- Paragenes --- Episomes - Congresses --- Plasmids - Congresses
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Plasmids --- Paragenes --- Cytoplasmic inheritance --- Genetic vectors --- Mobile genetic elements --- Extrachromosomal DNA --- Plasmids.
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Conjugation (Biology) --- Plasmids. --- Plasmids --- Paragenes --- Cytoplasmic inheritance --- Genetic vectors --- Mobile genetic elements --- Extrachromosomal DNA --- Reproduction --- Conjugation (Biology).
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Plasmids --- PLASMIDS --- Plasmids. --- Paragenes --- Cytoplasmic inheritance --- Genetic vectors --- Mobile genetic elements --- Extrachromosomal DNA --- Episome --- Plasmid --- Episomes --- DNA Transposable Elements --- Virophages
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This book captures in a single volume the wealth of information on the plasmid structure, function, and biology of all organisms that have been examined to date. Plasmids exhibit wide variations in size, modes of replication and transmission, host ranges, and the genes they carry and have provided us with a great understanding of basic life principles at the molecular level. Written by experts in the field, this book is a valuable source of up-to-date information, delivering the latest impacts on studies in the areas of plasmid types, genomes, purification analysis, and expression of recombinant proteins in bacteria. Plasmid utilization in the synthesis of plasmid-based vaccines, plasmids as genetic tools, and their applications in ecology and the evolutionary process are also covered. This book is a single source of valuable information for instructors and students in advanced undergraduate and graduate courses on plasmids. It will also appeal to researchers seeking to find new relationships between biological processes that are linked by plasmids to the environment.
Plasmids. --- Paragenes --- Cytoplasmic inheritance --- Genetic vectors --- Mobile genetic elements --- Extrachromosomal DNA --- Life Sciences --- Molecular Genetics --- Microbiology --- Genetics and Molecular Biology --- Biochemistry
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Plasmids --- -Gram negative bacteria --- Bacterial genetics --- Gram-negative bacteria --- Bacteria --- Bacteriology --- Microbial genetics --- Paragenes --- Cytoplasmic inheritance --- Genetic vectors --- Mobile genetic elements --- Extrachromosomal DNA --- Genetics --- Bacterial genetics. --- Gram-negative bacteria. --- Genetics. --- Plasmid genetics
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Bacterial diseases --- -Drug resistance in microorganisms --- -Plasmids --- -Paragenes --- Cytoplasmic inheritance --- Genetic vectors --- Mobile genetic elements --- Extrachromosomal DNA --- Antibiotic resistance in microorganisms --- Antibiotics resistance in microorganisms --- Bacterial resistance to antibiotics --- Drug resistance in micro-organisms --- Microbial drug resistance --- Resistance to drugs in microorganisms --- Microorganisms --- Bacterial infections --- Communicable diseases --- Medical bacteriology --- Pathogenic bacteria --- Congresses --- Effect of drugs on --- Drug resistance in microorganisms --- Plasmids --- Congresses. --- -Congresses --- Paragenes --- Drug resistance
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The yeast two-hybrid system is one of the most widely used and productive techniques available for investigating the macromolecular interactions that affect virtually all biological processes. In Two-Hybrid Systems: Methods and Protocols, Paul N. MacDonald has assembled a collection of these powerful molecular tools for examining and characterizing protein-protein, protein-DNA, and protein-RNA interactions. The techniques range from the most basic (introducing plasmids into yeasts, interaction assays, and recovering the plasmids from yeast) to the most advanced alternative strategies (involving one-hybrid, split two-hybrid, three-hybrid, membrane recruitment systems, and mammalian systems). Methods are also provided for dealing with the well-known problems of artifacts and false positives and for identifying the interacting partners in important biological systems, including the Smad and nuclear receptor pathways. To ensure ready reproducibility and robust results, each technique is described in step-by-step detail by researchers who employ it regularly. Comprehensive and highly practical, Two-Hybrid Systems: Methods and Protocols not only reveals how the great variety of plasmid vectors and approaches may be optimally deployed, but also quickly empowers novices to establish two-hybrid systems in their laboratories, and experienced researchers to expand their repertoire of techniques.
Protein binding --- Yeast fungi. --- Plasmids. --- Protéines --- Levures (Botanique) --- Plasmides --- Research --- Methodology. --- Fixation --- Recherche --- Méthodologie --- Protéines --- Méthodologie --- Paragenes --- Yeasts --- Binding, Protein --- Cytoplasmic inheritance --- Genetic vectors --- Mobile genetic elements --- Extrachromosomal DNA --- Fungi --- Basidiomycetes --- Blastomycetes --- Endomycetales --- Biochemistry --- Allosteric proteins --- Radioligand assay --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists
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Translocation (Genetics) --- Plasmids --- periodicals --- Extrachromosomal Inheritance. --- Plasmids. --- Plasmides --- Cytoplasmic Inheritance --- Extranuclear Inheritance --- Inheritance, Cytoplasmic --- Inheritance, Extrachromosomal --- Inheritance, Extranuclear --- Paragenes --- DNA, Chloroplast --- Cytoplasmic inheritance --- Genetic vectors --- Mobile genetic elements --- Extrachromosomal DNA --- Extrachromosomal Inheritance --- periodicals. --- Episome --- Plasmid --- Episomes --- Virophages --- DNA Transposable Elements --- Translocation, Genetic. --- Chromosomal Translocation --- Translocation, Chromosomal --- Chromosomal Translocations --- Genetic Translocation --- Genetic Translocations --- Translocations, Chromosomal --- Translocations, Genetic --- Gene Rearrangement --- Translocation, Genetic --- Periodical.
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Manipulation of recombinant DNA, which is almost exclusively performed using the host E. coli, constitutes one of the fundamental methodologies of molecular biotechnology. In E. coli Plasmid Vectors, experienced bench researchers describe their proven techniques for the manipulation of recombinant plasmids utilizing this popular bacterial host. The authors describe readily reproducible methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Also presented are methods for common downstream applications, such as mutagenesis, expression of recombinant proteins and RNA transcripts, and uses of reporter genes. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, tips on troubleshooting and avoiding known pitfalls and, where needed, a discussion of the interpretation and use of the results. Comprehensive and highly practical, E. coli Plasmid Vectors offers those new to the field a basic guide to the use of plasmid vectors in the cloning host E. coli, and those more experienced researchers a broad-ranging, proven array of successful techniques.
Plasmids --- Escherichia coli --- Genetic vectors --- Cloning vectors --- Expression vectors (Genetics) --- rDNA vectors --- Recombinant DNA vectors --- Vectors, Genetic --- Gene expression --- Molecular cloning --- Recombinant DNA --- E. coli (Bacterium) --- Escherichia --- Paragenes --- Cytoplasmic inheritance --- Mobile genetic elements --- Extrachromosomal DNA --- Biochemistry. --- Cytology. --- Biochemistry, general. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Composition
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