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Proteins act as macromolecular machinery that mediate many diverse biological processes - the molecular mechanisms of this machinery has fascinated biologists for decades. Analysis of the kinetic and thermodynamic features of these mechanisms could reveal unprecedented aspects of how the machinery function and will eventually lead to a novel understanding of various biological processes. This dissertation comprehensively demonstrates how two universally conserved guanosine triphosphatases in the signal recognition particle and its membrane receptor maintain the efficiency and fidelity of the co-translational protein targeting process essential to all cells. A series of quantitative experiments reveal that the highly ordered and coordinated conformational states of the machinery are the key to their regulatory function. This dissertation also offers a mechanistic view of another fascinating system in which multistate protein machinery closely control critical biological processes.

Guanosine triphosphatase. --- Protein binding. --- Chemistry --- Human Anatomy & Physiology --- Physical Sciences & Mathematics --- Health & Biological Sciences --- Organic Chemistry --- Animal Biochemistry --- G proteins --- Guanosine triphosphatase --- Biological transport. --- Physiological transport. --- GTP-binding proteins --- GTP regulatory proteins --- Guanine nucleotide-binding proteins --- Guanine nucleotide regulatory proteins --- Membrane transport --- Passive transport, Biological --- Physiological transport --- Transport, Biological --- GTP phosphohydrolase --- GTPase --- Guanosine triphosphate phosphohydrolase --- Guanosinetriphosphatase --- Chemistry. --- Analytical chemistry. --- Bioorganic chemistry. --- Biochemistry. --- Proteomics. --- Bioorganic Chemistry. --- Analytical Chemistry. --- Biochemistry, general. --- Molecular biology --- Proteins --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Medical sciences --- Bio-organic chemistry --- Biological organic chemistry --- Biochemistry --- Chemistry, Organic --- Analysis, Chemical --- Analytical chemistry --- Chemical analysis --- Metallurgical analysis --- Mineralogy, Determinative --- Physical sciences --- Composition --- Diffusion --- Osmosis --- Phosphatases --- Membrane proteins --- Analytical biochemistry. --- Analytic biochemistry --- Chemistry, Analytic --- Bioanalytic chemistry --- Bioanalytical chemistry --- Analytic chemistry

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Sustainable buildings. --- Ecologically sustainable buildings --- Environmentally sustainable buildings --- Green buildings (Green technology) --- Buildings --- Sustainable development

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This book is devoted to the optimum design of the DCT in a hybrid AC/DC microgrid, which takes into account not only the influence of different inductors/capacitors values, but also numerous design goals (i.e., VCG, efficiency, stability and so on). This book examines the DCT's design problem in detail. It begins by reviewing existing DCTs in, the hybrid AC/DC microgrid and their design problems. Following that, this book proposes a family of DCT optimization design approaches to ensure that the designed DCT has good power transmission and voltage regulation ability in the hybrid AC/DC microgrid, even when the actual inductors/capacitors values fluctuate with practical power and temperature. Following that, this book provides a family of multi-objective optimization design methodologies for the DCT to guarantee that it concurrently achieves the requirements of VCG, efficiency, and system stability. This book also covers how to control the DCT in a hybrid AC/DC microgrid optimally and generically.

Electric power production. --- Computational intelligence. --- Electronic circuits. --- Electrical Power Engineering. --- Computational Intelligence. --- Electronic Circuits and Systems. --- Electron-tube circuits --- Electric circuits --- Electron tubes --- Electronics --- Intelligence, Computational --- Artificial intelligence --- Soft computing --- Electric power generation --- Electricity generation --- Power production, Electric --- Electric power systems --- Electrification --- Electric currents, Direct. --- Electric transformers --- Design and construction. --- Transformers, Electric --- Electric currents --- Electric machinery --- Electric motors --- Currents, Direct --- Direct currents

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Analytical chemistry --- Organic chemistry --- Genetics --- General biochemistry --- organische chemie --- proteomics --- analytische chemie --- biochemie --- proteïnen