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Book
ISBN: 9811615365 9811615357 Year: 2021 Publisher: Singapore : Springer,
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Proteins --- Protein-protein interactions. --- Surface plasmon resonance. --- Analysis. --- Plasmon resonance, Surface --- Resonance, Surface plasmon --- Sensing, Surface plasmon resonance --- SPR (Surface plasmon resonance) --- Surface plasmon resonance sensing --- Biosensors --- Optical detectors --- Plasmons (Physics) --- Interactions, Protein-protein --- PPIs (Protein-protein interactions) --- Protein interactions --- Interactomes --- Molecular association --- Anàlisi de proteïnes --- Ressonància de plasmons superficials --- Ressonància de plasmònica de superficie --- Ressonància de plasmons de superficie --- Ressonància de plasmó superficial --- Detectors òptics --- Seqüència d'aminoàcids
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ISBN: 9789811615368 9789811615375 9789811615382 9789811615351 Year: 2021 Publisher: Singapore Springer Singapore, Imprint: Springer
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This volume describes methods and protocols for the fragment-based screening of proteins using Surface Plasmon Resonance (SPR). The initial chapter of the book discusses the principle of SPR for the identification of biomolecular interactions, while the subsequent chapters introduce methods for labelling proteins with different tags including, histidine and biotin tags. It also discusses techniques and factors that affect the amine and biotin-streptavidin coupling and methods to optimize the interactions. Next, it describes fragment preparation for screening in SPR and presents methods to calculate equilibrium dissociation constant (KD) and ligand efficiency (LE). It reviews techniques of next-generation injections that improve the efficiency of the characterization process over traditional SPR by determining the kinetics and affinity in a single step. Finally, the book elucidates a comprehensive yet representative description of challenges associated with the molecular interaction of proteins using SPR.
Chemistry --- General biophysics --- History of human medicine --- biofysica --- farmacologie --- proteïnen
Book
ISBN: 9789811615368 9789811615375 9789811615382 9789811615351 Year: 2021 Publisher: Singapore Springer Singapore :Imprint: Springer
Abstract | Keywords | Export | Availability | Bookmark
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- Reference Manager
- EndNote
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Chemistry --- General biophysics --- History of human medicine --- biofysica --- farmacologie --- proteïnen
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