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Molecular biology --- Analytical biochemistry --- Molecular cloning --- Polymerase chain reaction --- Clonage moléculaire --- Réaction en chaîne de la polymérase --- Laboratory manuals --- Manuels de laboratoire --- Chain reaction, Polymerase --- PCR (Biochemistry) --- Polymerization --- DNA polymerases --- Cloning, Molecular --- DNA cloning --- Gene cloning --- Cloning --- Genetic engineering --- Molecular genetics --- Clone cells --- Clonage moléculaire --- Réaction en chaîne de la polymérase
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PCR has been successfully utilized in every facet of basic, cli- cal, and applied studies of the life sciences, and the impact that PCR has had on life science research is already staggering. C- comitant with the essentially universal use of PCR has been the creative and explosive development of a wide range of PCR-based techniques and applications. These increasingly numerous pro- cols have each had the general effect of facilitating and acceler- ing research. Because PCR technology is relatively easy and inexpensive, PCR applications are well within the reach of every research lab. In this sense, PCR has become the "equalizer" between "small" and "big" labs, since its use makes certain projects, especially those related to molecular cloning, now far more feasible for the small lab with a modest budget. This new volume on PCR Protocols does not attempt the impossible task of representing all PCR-based protocols. Rather, it presents a range of protocols, both analytical and preparative, that provide a solid base of knowledge on the use of PCR in many c- mon research problems. The first six chapters provide some basic information on how to get started. Chapters 7-19 represent primarily analytical uses of PCR, both for simple DNA and RNA detection, as well as for more complex analyses of nucleic acid (e. g. , DNA footprin ting, RNA splice site localization). The remaining chapters represent "synthetic," or preparative, uses of PCR.
Molecular biology --- Polymerase Chain Reaction --- Polymerase chain reaction --- Réaction en chaîne de la polymérase --- methods --- Methodology --- Méthodologie --- Electronic books. -- local. --- Polymerase chain reaction -- Methodology. --- Polymerization -- Methodology. --- Nucleic Acid Amplification Techniques --- Genetic Techniques --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Animal Biochemistry --- Human Anatomy & Physiology --- Health & Biological Sciences --- -57.088 --- 577.2 --- Chain reaction, Polymerase --- PCR (Biochemistry) --- Polymerization --- DNA polymerases --- methods. --- Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- Molecular bases of life. Molecular biology --- Methodology. --- 577.2 Molecular bases of life. Molecular biology --- 57.088 Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- Réaction en chaîne de la polymérase --- Méthodologie --- 57.088 --- Polymerisation --- Polymers --- Polymers and polymerization --- Synthesis of polymers --- Chemical reactions --- Synthesis --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Polymerase chain reaction - Methodology --- Polymerase Chain Reaction - methods
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The advent of PCR, with its power to amplify tiny amounts of DNA, quickly spawned the development of many analytical procedures that are widely used for detection, measurement, and characterization. However, creative investigators soon discovered the power of PCR for synthetic or preparative uses. This volume focuses on such preparative PCR protocols, which can be used in the cloning and modification of DNA. PCR Cloning Protocols: From Molecular Cloning to Genetic Engineer ing is divided into seven parts, each containing a collection of chapters address ing a general approach or goal. Part I presents basic PCR protocols, emphasizing optimizing conditions for (he amplification of DNA fragments of several kilobases in length. Part 11 offers several procedures for cloning PCR prod ucts, depending on whether a specific restriction site can be used in the clon ing vector, the PCR product is to be gel purified before cloning, or the fragmeni needs to be inserted in one or both orientations. Part III includes several pro tocols involved in the mutagenesis of DNA, either site-directed or not, as well as several approaches to recombinant PCR, either for mutagenesis or building a custom gene, as well as one chapter describing a specific use of in vitro selection. Part IV addresses the frequent need to amplify and clone segments of DNA that are to the right, left, or scattered within a stretch of DNA (either vector, chromosomal, or cDNA) of known sequence.
Life sciences. --- Biochemistry. --- Life Sciences. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Biosciences --- Sciences, Life --- Science --- Composition
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Endocrine and Reproductive Physiology, a volume in the Mosby Physiology Monograph Series, explains the fundamentals of endocrine and reproductive physiology in a clear and concise manner. This medical textbook gives you a basic understanding of how endocrine and metabolic physiology affects other body systems in health and disease, including the clinical dimensions of reproductive endocrinology. Bridge the gap between normal function and disease with pathophysiology content throughout the book. Easily master the material in your systems-based curricu
Glandular physiology --- Fertiliteit --- Endocrinologie --- Fysiologie --- Endocrine Glands --- Reproductive Physiological Phenomena. --- physiology. --- Vruchtbaarheid --- Endocrine glands --- Reproduction. --- Physiology. --- Amphimixis --- Generation --- Pangenesis --- Procreation --- Biology --- Life (Biology) --- Physiology --- Sex (Biology) --- Embryology --- Generative organs --- Theriogenology --- Ductless glands --- Gland of internal secretion --- Glands, Ductless --- Glands --- Chromaffin cells --- Hormones --- Reproduction --- physiology --- Reproductive Physiological Phenomena --- Endocrine Glands - physiology
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Endocrine and Reproductive Physiology, a volume in the Mosby Physiology Monograph Series, explains the fundamentals of endocrine and reproductive physiology in a clear and concise manner. This medical textbook gives you a basic understanding of how endocrine and metabolic physiology affects other body systems in health and disease, including the clinical dimensions of reproductive endocrinology. Bridge the gap between normal function and disease with pathophysiology content throughout the book. Easily master the material in your systems-based curriculum with learning objectives, Clinical Concept boxes, chapter summaries, and self-study questions. Understand complex concepts by examining almost 200 clear, 2-color diagrams. Apply what you've learned to real-life clinical situations using featured clinical commentaries.
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Outside back cover : "Gain a foundational understanding of how endocrine and metabolic physiology affects other body systems in health and disease, including the clinical dimensions of reproductive endocrinology. Endocrine and Reproductive Physiology, a volume in the Mosby Physiology Series, explains the fundamentals of this complex subject in a clear and concise manner, while helping you bridge the gap between normal function and disease with pathophysiology content throughout the book."
Endocrine Glands --- Reproductive Physiological Phenomena --- Endocrine System Diseases --- physiology --- Endocrine glands --- Reproduction --- Physiology. --- Reproductive Physiological Phenomena. --- Glandular physiology --- Endocrine Glands - physiology --- Endocrine System Diseases - examination questions
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