Listing 1 - 7 of 7
Sort by
PCR cloning protocols : from molecular cloning to genetic engineering
Author:
ISBN: 0896033430 0896034436 Year: 1997 Volume: 67 Publisher: Totowa Humana press

PCR protocols: current methods and applications
Author:
ISBN: 0896032442 1592595022 9780896032446 Year: 1993 Volume: 15 Publisher: Totowa, N.J. Humana Press

Loading...
Export citation

Choose an application

Bookmark

Abstract

PCR has been successfully utilized in every facet of basic, cli- cal, and applied studies of the life sciences, and the impact that PCR has had on life science research is already staggering. C- comitant with the essentially universal use of PCR has been the creative and explosive development of a wide range of PCR-based techniques and applications. These increasingly numerous pro- cols have each had the general effect of facilitating and acceler- ing research. Because PCR technology is relatively easy and inexpensive, PCR applications are well within the reach of every research lab. In this sense, PCR has become the "equalizer" between "small" and "big" labs, since its use makes certain projects, especially those related to molecular cloning, now far more feasible for the small lab with a modest budget. This new volume on PCR Protocols does not attempt the impossible task of representing all PCR-based protocols. Rather, it presents a range of protocols, both analytical and preparative, that provide a solid base of knowledge on the use of PCR in many c- mon research problems. The first six chapters provide some basic information on how to get started. Chapters 7-19 represent primarily analytical uses of PCR, both for simple DNA and RNA detection, as well as for more complex analyses of nucleic acid (e. g. , DNA footprin ting, RNA splice site localization). The remaining chapters represent "synthetic," or preparative, uses of PCR.


Book
PCR Cloning Protocols : From Molecular Cloning to Genetic Engineering
Author:
ISBN: 1280836741 9786610836741 1592595537 0896034836 Year: 1997 Publisher: Totowa, NJ : Humana Press : Imprint: Humana Press,

Loading...
Export citation

Choose an application

Bookmark

Abstract

The advent of PCR, with its power to amplify tiny amounts of DNA, quickly spawned the development of many analytical procedures that are widely used for detection, measurement, and characterization. However, creative investigators soon discovered the power of PCR for synthetic or preparative uses. This volume focuses on such preparative PCR protocols, which can be used in the cloning and modification of DNA. PCR Cloning Protocols: From Molecular Cloning to Genetic Engineer­ ing is divided into seven parts, each containing a collection of chapters address­ ing a general approach or goal. Part I presents basic PCR protocols, emphasizing optimizing conditions for (he amplification of DNA fragments of several kilobases in length. Part 11 offers several procedures for cloning PCR prod­ ucts, depending on whether a specific restriction site can be used in the clon­ ing vector, the PCR product is to be gel purified before cloning, or the fragmeni needs to be inserted in one or both orientations. Part III includes several pro­ tocols involved in the mutagenesis of DNA, either site-directed or not, as well as several approaches to recombinant PCR, either for mutagenesis or building a custom gene, as well as one chapter describing a specific use of in vitro selection. Part IV addresses the frequent need to amplify and clone segments of DNA that are to the right, left, or scattered within a stretch of DNA (either vector, chromosomal, or cDNA) of known sequence.


Book
Endocrine and reproductive physiology
Authors: ---
ISBN: 9780323087049 9780323088282 0323088287 0323087043 Year: 2013 Publisher: Elsevier Mosby

Loading...
Export citation

Choose an application

Bookmark

Abstract

Endocrine and Reproductive Physiology, a volume in the Mosby Physiology Monograph Series, explains the fundamentals of endocrine and reproductive physiology in a clear and concise manner. This medical textbook gives you a basic understanding of how endocrine and metabolic physiology affects other body systems in health and disease, including the clinical dimensions of reproductive endocrinology. Bridge the gap between normal function and disease with pathophysiology content throughout the book. Easily master the material in your systems-based curricu


Digital
Endocrine and reproductive physiology
Authors: ---
ISBN: 9780323087049 9780323088282 0323088287 Year: 2013 Publisher: Philadelphia, Pa Elsevier

Loading...
Export citation

Choose an application

Bookmark

Abstract

Endocrine and Reproductive Physiology, a volume in the Mosby Physiology Monograph Series, explains the fundamentals of endocrine and reproductive physiology in a clear and concise manner. This medical textbook gives you a basic understanding of how endocrine and metabolic physiology affects other body systems in health and disease, including the clinical dimensions of reproductive endocrinology. Bridge the gap between normal function and disease with pathophysiology content throughout the book. Easily master the material in your systems-based curriculum with learning objectives, Clinical Concept boxes, chapter summaries, and self-study questions. Understand complex concepts by examining almost 200 clear, 2-color diagrams. Apply what you've learned to real-life clinical situations using featured clinical commentaries.

Endocrine physiology
Authors: ---
ISBN: 9780323036665 Year: 2007 Publisher: Philadelphia, Pa Mosby/Elsevier

Loading...
Export citation

Choose an application

Bookmark

Abstract


Book
Endocrine and reproductive physiology
Authors: --- ---
ISBN: 9780323595735 0323595731 Year: 2019 Publisher: St.Louis, Mo. Elsevier

Loading...
Export citation

Choose an application

Bookmark

Abstract

Outside back cover : "Gain a foundational understanding of how endocrine and metabolic physiology affects other body systems in health and disease, including the clinical dimensions of reproductive endocrinology. Endocrine and Reproductive Physiology, a volume in the Mosby Physiology Series, explains the fundamentals of this complex subject in a clear and concise manner, while helping you bridge the gap between normal function and disease with pathophysiology content throughout the book."

Listing 1 - 7 of 7
Sort by