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PCR (Polymerase Chain Reaction) technology has become an indispensable component of routine veterinary diagnostics. However, a number of pitfalls and limiting factors affect its sensitivity and specificity of detection. It is imperative that veterinary PCR diagnosticians include such considerations in their work. Extensive experience with PCR technology in both research and diagnostic applications enables researchers to pinpoint these practical limitations, and therefore instruct the user in approaches that avoid these common errors.This E-book discusses the basic concepts, chemistries, and in
Polymerase chain reaction --- Polymerase chain reaction. --- DNA probes. --- Microorganisms --- Deoxyribonucleic acid probes --- Gene probes --- Genomic probes --- Probes, DNA --- Deoxyribonucleotides --- Nucleic acid probes --- Chain reaction, Polymerase --- PCR (Biochemistry) --- Polymerization --- DNA polymerases --- Molecular diagnosis --- Diagnostic use.
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