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During the 20th century Xenopus has flourished as an accessible experimental model system for studying vertebrate biology. The second edition of  Xenopus Protocols:Post-Genomic Approaches expands upon the previous edition with novel approaches inspired by Xenopus tropicalis and the sequencing of its genome. Xenopus tropicalis genomics and tractable genetics combined with the unique experimental advantages of Xenopus laevis and their distinctive phylogenetic position make them unique model systems for vertebrate embryonic development in the 21st century. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Xenopus Protocols: Post-Genomic Approaches, Second Edition provides a toolkit that will enable every laboratory to maximize the power of this extraordinary experimental system in the post-genomic age.  .

Xenopus --- Investigative Techniques --- Pipidae --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Anura --- Amphibians --- Vertebrates --- Chordata --- Animals --- Eukaryota --- Organisms --- Genetic Techniques --- Zoology --- Health & Biological Sciences --- Embryology --- Laboratory manuals --- Embriologie --- Manuels de laboratoire --- Bombina --- Frogs and Toads --- Salientia --- Toad, Fire-Bellied --- Toads and Frogs --- Anuras --- Fire-Bellied Toad --- Fire-Bellied Toads --- Salientias --- Toad, Fire Bellied --- Toads, Fire-Bellied --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Genetics --- Eucarya --- Eukarya --- Eukaryotes --- Eukaryotas --- Eukaryote --- Animalia --- Animal --- Metazoa --- Chordates --- Chordate --- Vertebrate --- Amphibia --- Amphibian --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- African clawed frog --- Dactylethra --- Silurana --- methods --- Medicine. --- Human genetics. --- Animal genetics. --- Animal models in research. --- Biomedicine. --- Human Genetics. --- Animal Genetics and Genomics. --- Animal Models. --- Animal experimentation --- Biological models --- Laboratory animals --- Research --- Heredity, Human --- Human biology --- Physical anthropology --- Clinical sciences --- Medical profession --- Life sciences --- Medical sciences --- Pathology --- Physicians --- Laboratory animals. --- Animals, Experimental --- Animals, Laboratory --- Animals in research --- Experimental animals --- Lab animals --- Animal culture --- Laboratory organisms --- Working animals

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Organogenesis of the kidney has been intensely studied for over a century. In recent years advances in molecular techniques have not only made great inroads into exploring the genetic regulation of this complex process but also began to unravel the molecular basis of many forms of congenital kidney disease. This book is a comprehensive study on these findings and the only book available with such in depth coverage of the kidney. Key Features * Hundreds of color figures depicting key events in all aspects of kidney development * Full coverage of the genetic and cellular basis of kidney development * Analysis of the genetic basis of the major congenital kidney diseases.

Nefrology