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Adult Stem Cells, second edition, takes a critical look at issues concerning the  developmental or differentiation potential for a variety of tissue types and for specific adult stem cell types. Since the first edition appeared a decade ago, our understanding of adult stem cells, and more specifically tissue-specific adult stem cells, has advanced tremendously. And an increased interest in regenerative medicine and potential stem cell applications  has driven a quest for better understanding of stem cell biology. In turn, this has spawned much activity on generation and utilization of more and better reagents to identify and isolate stem cells and stem cell-like subpopulations, and on assays elucidating their developmental or differentiation potential and functional integration with host tissues and organs. In this fully updated new edition, chapters cover topics ranging from signaling pathways maintaining stemness in hematopoietic cells to regeneration after injury and endocrine mechanisms underlying the stem cell theory of aging. Other chapters cover  stem cells by organ or system including pituitary, cardiac, epithelial, teeth, lung, ovary, prostate, liver, and many more.  Importantly, the authors of the chapters have not only summarized their successes, but have also summarized some of the difficulties that each particular field is still facing with respect to maximizing the utility of stem cells in clinical settings. Collectively, they impart both the excitement and challenges facing stem cell utilization for repair and regeneration  making this book essential reading for those involved in stem cell research as well as those involved in clinical assays.

Stem cells --- Cell differentiation --- Industrial applications. --- Molecular aspects. --- Life sciences. --- Biotechnology. --- Cell biology. --- Stem cells. --- Life Sciences. --- Stem Cells. --- Cell Biology. --- Colony-forming units (Cells) --- Mother cells --- Progenitor cells --- Cells --- Cell biology --- Cellular biology --- Biology --- Cytologists --- Chemical engineering --- Genetic engineering --- Biosciences --- Sciences, Life --- Science --- Molecular biology --- Cytology.

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In recent years our ability to understand and manipulate epidermal cells has increased tremendously, opening significant new possibilities in both basic science research and in regenerative medicine, including wound healing and transplantation. In Epidermal Cells: Methods and Protocols, a panel of experienced basic and clinical researchers describe in step-by-step detail the powerful methods they have developed and optimized to analyze and manipulate epidermal cell precursors and mature epidermal cells. These protocols cover different methods and models for culturing epidermal cells, for enriching very early epidermal progenitors, as well as for studying epidermal cell commitment and differentiation both in vitro and in vivo. Topics of special interest include the derivation, characterization, and utility of epidermal stem cells, mature epidermal cells and their characterization, and applications in regenerative medicine. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Epidermal Cells: Methods and Protocols offers experienced and novice investigators alike an invaluable collection of readily reproducible techniques designed to broaden not only our understanding of the biology of epidermal cells, but also their utility in normal tissue homeostasis and regenerative medicine applications.

Epidermis --- Cell Culture Techniques --- Cytological Techniques --- Genetic Engineering --- Keratinocytes --- Organ Culture Techniques --- Physiology --- Research --- Methodology. --- Cytology --- cytology. --- methods. --- Cell Culture --- Organ Culture --- Methodology --- cytology --- methods --- Electronic books. -- local. --- Epidermis -- Cytology -- Research -- Methodology. --- Epidermis -- Physiology -- Research -- Methodology. --- Methods --- Epithelial Cells --- Epithelium --- Culture Techniques --- Investigative Techniques --- Clinical Laboratory Techniques --- Genetic Techniques --- Skin --- Cells --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Tissues --- Integumentary System --- Anatomy --- Human Anatomy & Physiology --- Health & Biological Sciences --- Internal medicine. --- Cytology. --- Internal Medicine. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cytologists --- Medicine, Internal --- Medicine --- Epidermis - Physiology - Research - Methodology --- Epidermis - Cytology - Research - Methodology --- Epidermis - cytology --- Cell Culture - methods --- Cytological Techniques - methods --- Genetic Engineering - methods --- Keratinocytes - cytology --- Organ Culture - methods --- Acqui 2006

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This volume explores laser-assisted bioprinting, focusing on the most recent developments in its use for  tissue engineering. Bringing together authoritative and international perspectives, the text begins with an overview and goes on to cover bioprinting in cell viability and pattern viability, tissue microfabrication to study cell proliferation, microenvironment for controlling stem cell fate, cell differentiation, zigzag cellular tubes, cartilage tissue engineering, osteogenesis, vessel substitutes, skin tissue and much more. Bioprinting is on its way to becoming a dominant technology in tissue-engineering; Bioprinting in Regenerative Medicine, from the bestselling Stem Cell Biology in Regenerative Medicine series, is essential reading for those researching or working in regenerative medicine, tissue engineering, or translational research. Those studying or working with stem cells who are interested in the development of the field will also find the information invaluable.

Regenerative Medicine --- Tissue Engineering --- Culture Techniques --- Medicine --- Health Occupations --- Clinical Laboratory Techniques --- Investigative Techniques --- Disciplines and Occupations --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Regenerative medicine. --- Tissue engineering. --- Stem cells. --- Biomedical engineering. --- Clinical engineering --- Medical engineering --- Colony-forming units (Cells) --- Mother cells --- Progenitor cells --- Life sciences. --- Life Sciences. --- Regenerative Medicine/Tissue Engineering. --- Stem Cells. --- Biomedical Engineering. --- Bioengineering --- Biophysics --- Engineering --- Cells --- Biomedical engineering --- Regenerative medicine --- Tissue culture --- Regeneration (Biology) --- Biomedical Engineering and Bioengineering.

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This latest volume in Springer's Stem Cell Biology and Regenerative Medicine series looks at the state-of-the-science in stem cells, discusses the current challenges, and examines the new directions the field is taking. Dr. Turksen, Editor-in-Chief of the journal Stem Cell Reviews and Reports, has assembled a volume of internationally-known scientists who cover topics that are both research and clinically oriented. The contents range from sources of stem cells through their physiological role in health and disease, therapeutic applications in regenerative medicine and ethics and society. An initial overview and a final summary bookend the contents into a cohesive and invaluable volume.

Stem cells -- Transplantation. --- Stem cells. --- Stem cells --- Investigative Techniques --- Cell Transplantation --- Medicine --- Transplantation --- Health Occupations --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Disciplines and Occupations --- Surgical Procedures, Operative --- Stem Cell Research --- Stem Cell Transplantation --- Regenerative Medicine --- Biology --- Health & Biological Sciences --- Cytology --- Therapeutic use --- Oncology. --- Colony-forming units (Cells) --- Mother cells --- Progenitor cells --- Life sciences. --- Cancer research. --- Reproductive medicine. --- Life Sciences. --- Stem Cells. --- Cancer Research. --- Reproductive Medicine. --- Cells --- Human reproduction --- Human reproductive health --- Human reproductive medicine --- Reproductive medicine --- Health --- Cancer research --- Biosciences --- Sciences, Life --- Science --- Health aspects --- Tumors

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Much research has focused on the basic cellular and molecular biological aspects of stem cells. Much of this research has been fueled by their potential for use in regenerative medicine applications, which has in turn spurred growing numbers of translational and clinical studies. However, more work is needed if the potential is to be realized for improvement of the lives and well-being of patients with numerous diseases and conditions. With a goal to accelerate advances by timely information exchange, this new book series 'Cell Biology and Translational Medicine (CBTMED)' as part of SpringerNature's longstanding and very successful Advances in Experimental Medicine and Biology book series is launched. Emerging areas of regenerative medicine and translational aspects of stem cells will be covered in each volume. Outstanding researchers are recruited to highlight developments and remaining challenges in both the basic research and clinical arenas. This current book is the first volume of a continuing series.

Stem cells. --- Regenerative medicine. --- Gene therapy. --- Genetic engineering. --- Stem Cells. --- Regenerative Medicine/Tissue Engineering. --- Gene Therapy. --- Genetic Engineering. --- Colony-forming units (Cells) --- Mother cells --- Progenitor cells --- Cells --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Therapy, Gene --- Genetic engineering --- Therapeutics --- Tissue engineering. --- Biomedical engineering --- Regenerative medicine --- Tissue culture --- Medicine --- Regeneration (Biology)