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Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The authors provide readily reproducible techniques for the generation of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer. Of immense benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones.

Cell Nucleus --- Cloning, Organism --- Cell Differentiation --- Gene Transfer Techniques. --- Cell nuclei --- Gene therapy. --- Transgenic animals. --- Thérapie génique --- Animaux transgéniques --- transplantation. --- methods. --- genetics. --- physiology. --- Transplantation. --- Gene therapy --- Transgenic animals --- Gene Transfer Techniques --- Transplantation --- methods --- genetics --- Cell nuclei -- Transplantation. --- Investigative Techniques --- Surgical Procedures, Operative --- Genetic Techniques --- Publication Formats --- Cell Physiological Processes --- Biology --- Biological Science Disciplines --- Reproductive Techniques --- Intracellular Space --- Organelles --- Cell Physiological Phenomena --- Publication Characteristics --- Cytoplasmic Structures --- Natural Science Disciplines --- Cellular Structures --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Disciplines and Occupations --- Cells --- Cytoplasm --- Phenomena and Processes --- Anatomy --- Laboratory Manuals --- Methods --- Physiology --- Genetics --- Medicine --- Health & Biological Sciences --- Cytology --- Pathology --- Therapy, Gene --- Nuclear transfer --- Nuclear transplantation --- Transplantation of cell nuclei --- Life sciences. --- Cell biology. --- Life Sciences. --- Cell Biology. --- Cell biology --- Cellular biology --- Cytologists --- Biosciences --- Sciences, Life --- Science --- Animals --- Transgenic organisms --- Animal genetic engineering --- Genetic engineering --- Therapeutics --- Reproduction --- Cytology. --- Cell nuclei - Transplantation --- Cloning, Organism - methods - laboratory manuals --- Cell Differentiation - genetics - laboratory manuals --- Gene Transfer Techniques - laboratory Manuals