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RNA interference (RNAi) is a biological process in which small nucleotide sequences destroy specific mRNA molecules and, therefore, interrupt genetic expression. RNAi has rapidly developed into a widely used technique in biotechnology, experimental biology, genomics and medicine. Frontiers in RNAi is a collection of research articles covering the applications of RNAi principles and technologies and has been compiled by members of the RNAi Global Initiative, a collection of academic and industrial groups with extensive experience in genome-wide RNAi screening. Chapters included in the first volume touch on some of the recent advances in RNAi technology (i.e., applications to 3D cell culture, drug repurposing, and bioproduction) and provide guidance on how to address the continued challenges associated with off-target effects, database structuring, and pooled shRNA screens.
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This volume discusses the interactions between viruses and their host cells, and explores the roles of host and viral genes and non-coding RNAs in the virus replication cycle. During infection, viruses express a variety of genes, encoding proteins and RNAs that serve to subjugate the cell – by redirecting cellular processes to support viral replication and, at the same time, by mitigating the cellular response to infection. In this book, experts discuss these interactions in depth, and elaborate on our current understanding of virus-cell interactions for a diverse range of viruses, including positive and negative sense RNA viruses, DNA viruses, and a vector-borne virus. The roles of non-coding RNAs are also discussed. While each class of viruses has distinct replication requirements, this volume reveals unique features and commonalities in viral replication cycles. Accordingly, it represents a valuable source of information for researchers and clinicians alike.
Viral genetics --- Virus genetics --- Viruses --- Microbial genetics --- Mathematical models. --- Genetics --- Medical virology. --- Emerging infectious diseases. --- Virology. --- Infectious Diseases. --- Emerging infections --- New infectious diseases --- Re-emerging infectious diseases --- Reemerging infectious diseases --- Communicable diseases --- Medical microbiology --- Virology --- Virus diseases --- RNA, Untranslated --- Virus Replication --- Host Microbial Interactions --- Virus Diseases --- genetics --- Bacteria Host Interactions --- Bacterial-Host Interactions --- Bacterium-Host Interactions --- Host Bacteria Interactions --- Host Microbe Interactions --- Host Microbiota Interactions --- Host Virus Interactions --- Host-Fungal Interactions --- Host-Microbial Interface --- Microbe Host Interactions --- Microbial Host Interactions --- Microbiota Host Interactions --- Viral-Host Interactions --- Virus Host Interactions --- Bacteria Host Interaction --- Bacterial Host Interactions --- Bacterial-Host Interaction --- Bacterium Host Interactions --- Bacterium-Host Interaction --- Host Bacteria Interaction --- Host Fungal Interactions --- Host Microbe Interaction --- Host Microbial Interaction --- Host Microbial Interface --- Host Microbiota Interaction --- Host Virus Interaction --- Host-Fungal Interaction --- Host-Microbial Interfaces --- Microbe Host Interaction --- Microbial Host Interaction --- Microbiota Host Interaction --- Viral Host Interactions --- Viral-Host Interaction --- Virus Host Interaction --- Noncoding RNA --- RNA, Non-Coding --- RNA, Non-Peptide-Coding --- RNA, Non-Protein-Coding --- RNA, Noncoding --- RNA, Nontranslated --- npcRNA --- Non-Coding RNA --- Non-Peptide-Coding RNA --- Non-Protein-Coding RNA --- Nontranslated RNA --- RNA, Non Coding --- RNA, Non Peptide Coding --- RNA, Non Protein Coding --- Untranslated RNA --- Infectious diseases. --- Microbiology
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This volume summarizes current techniques; ranging from protocols for virus growth, isolation, quantification and generation of recombinant RSV virus to procedures for the efficient characterization of the host immune response to RSV infection. These techniques are used in numerous laboratories around the world and are the building blocks that support the majority of RSV virus research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Humana Respiratory Syncytial Virus: Methods and Protocols aims to ensure successful results in the further study of this vital field.
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This volume discusses the interactions between viruses and their host cells, and explores the roles of host and viral genes and non-coding RNAs in the virus replication cycle. During infection, viruses express a variety of genes, encoding proteins and RNAs that serve to subjugate the cell – by redirecting cellular processes to support viral replication and, at the same time, by mitigating the cellular response to infection. In this book, experts discuss these interactions in depth, and elaborate on our current understanding of virus-cell interactions for a diverse range of viruses, including positive and negative sense RNA viruses, DNA viruses, and a vector-borne virus. The roles of non-coding RNAs are also discussed. While each class of viruses has distinct replication requirements, this volume reveals unique features and commonalities in viral replication cycles. Accordingly, it represents a valuable source of information for researchers and clinicians alike.
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