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This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers recent research and methods development for changing the DNA sequence within the genomes of cells and organisms. Focusing on enzymes that generate double-strand breaks in DNA, the chapters describe use of molecular tools to introduce or delete genetic information at specific sites in the genomes of animal, plant and bacterial cells.Continues the legacy of this premier serial with quality chapters authored by leaders in the fieldCovers r
Esterases --- Computational Biology --- DNA Restriction-Modification Enzymes --- Endodeoxyribonucleases --- Genetic Techniques --- Genetics --- Deoxyribonucleases --- Enzymes --- Hydrolases --- Biology --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Biological Science Disciplines --- Enzymes and Coenzymes --- Natural Science Disciplines --- Chemicals and Drugs --- Disciplines and Occupations --- DNA Restriction Enzymes --- Genomics --- Endonucleases --- Genetic Engineering --- Health & Biological Sciences --- Genomics. --- Genome research --- Genomes --- Research --- Molecular genetics
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This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. Methods to assess mitochondrial function is of great interest to neuroscientists studying chronic forms of neurodegeneration, including Parkinson's, Alzheimer's, ALS, Huntington's and other triplet repeat diseases, but also to those working on acute conditions such as stroke and traumatic brain injury. This volume covers research methods on how to assess the life cycle of mitochondria including trafficking, fusion, fission, and degradation. Multiple persp
Mitochondria. --- Phosphoprotein phosphatases. --- Protein kinases. --- Subcellular Fractions --- Nervous System --- Cells --- Organelles --- Metabolic Diseases --- DNA, Circular --- Cytoplasmic Structures --- DNA --- Cellular Structures --- Anatomy --- Nutritional and Metabolic Diseases --- Diseases --- Nucleic Acids --- Cytoplasm --- Intracellular Space --- Nucleic Acids, Nucleotides, and Nucleosides --- Chemicals and Drugs --- Mitochondria --- Mitochondrial Diseases --- DNA, Mitochondrial --- Neurons --- Protein kinase --- Protein phosphotransferases --- Phosphoprotein phosphohydrolases --- Protein phosphatases --- Protein phosphoesterases --- Protein phosphohydrolases --- Chondriosomes --- Phosphotransferases --- Phosphatases --- Cell organelles --- Protoplasm
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"Since their discovery and subsequent development into laboratory tools, CRISPR Cas systems have revolutionized the science of gene editing and their possible applications continue to expand, from basic research to potentially groundbreaking medical and commercial uses. Led by a distinguished team of editors, CRISPR Biology and Applications explores the subject matter needed to delve into this fascinating area. This comprehensive text presents the diversity of CRISPR Cas systems, the underlying biology of these systems, and CRISPR based technologies and applications".
CRISPR (génétique). --- Génétique microbienne. --- Protéines de liaison à l'ARN. --- Microbial genetics. --- CRISPR-Cas Systems. --- Genetics, Microbial. --- RNA-Binding Proteins.
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