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In this work, different frameworks designed for aiding in the automatic generation and simulation of resource allocation models of cellular metabolism are explored. Their main characteristics and underlying mathematics are outlined, and a comparison between them is performed. To perform this comparison the same minimal metabolic model was implemented in all frameworks, and the results of the model simulation in each one are presented. Details of the implementation and the necessary changes to couple the minimal model used with the different modeling approaches implemented in the frameworks are also presented. Finally, general guidelines for framework selection are produced, and condensed into a decision tree.
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