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This volume provides easily accessible and comprehensive collection of methods, techniques, and strategies to investigate the molecular and cellular biology of peroxisomes in different organisms. Chapters detail valuable instructions, guidelines and protocols for molecular cell biologists, biochemists and biomedical researchers with an interest in peroxisome biology. Chapters in Peroxisomes: Methods and Protocols illustrate the isolation of peroxisomes, investigation of properties of membrane proteins, protocols to investigate and manipulate peroxisomes in cellular systems, detection of peroxisomes, including immunofluorescence, cytochemistry, cryo-immuno electron microscopy, and live cell imaging approaches. Authoritative and practical, Peroxisomes: Methods and Protocols aims to be useful for those already working on peroxisomes as well as for those who would like to start working on this fascinating organelle.
Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists
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Das Buch ist als eine kompakte Einführung in die Physikalische Chemie für Nicht-Chemiker in Bachelor-Studiengängen geschrieben worden. Dies können vor allem angehende Biotechnologen, aber auch Chemieingenieure, Bioinformatiker oder andere Biowissenschaftler bzw. -ingenieure sein. Zudem sollte es auch für Chemiker als Einstiegslektüre gut zu nutzen sein. Die wichtigsten Grundlagen und die dazu nötige Mathematik werden dargestellt, inklusive einiger für das Verständnis wichtiger Herleitungen. Darüber hinaus kommen im Text und in eigenen Kapiteln viele Beispiele und Anwendungen in den Hauptfokus. Es wird gezeigt, wie die Physikalische Chemie zum Verständnis beiträgt und praktische Lösungen liefert. Dazu wird auch ein wenig der historische Bezug gebildet; im Vordergrund steht aber die notwendige Basis, um dieses vielfältige und anspruchsvolle Fach erfolgreich zu verstehen, insbesondere in den Biowissenschaften und der Chemie. Ein Kapitel beschäftigt sich mit Informationen zu physikalisch-chemischen Praktika, die oft an solche Lehrveranstaltungen gekoppelt sind. Abgerundet wird das Buch durch Verständnisfragen, Übungen und Dateien, die Formeln, Tabellen und Abbildungen zum Leben erwecken. Der Autor Michael Schrader ist seit über 10 Jahren Professor für Physikalische Chemie und Instrumentelle Analytik an der Hochschule Weihenstephan-Triesdorf. Davor war er 10 Jahre in der Biotechnologie tätig und hat an der Universität Hannover in Physikalischer Chemie promoviert.
Physical chemistry. --- Chemistry. --- Electrochemistry. --- Physics. --- Life sciences. --- Physical Chemistry. --- Chemistry/Food Science, general. --- Physics, general. --- Life Sciences, general.
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This volume describes protocols for basic state-of-the-art approaches in the field of peptidomics. Most of these approaches are independent of the instruments used for analysis and can easily be adapted for equipment that is available in a typical proteomics facility. Chapters detail many of the basic techniques used to detect and identify peptides, methods for the relative quantitation of peptides between samples using isotopic labels or label-free approaches, and biological species as well as sample types. Written in the highly successful format of the Methods in Molecular Biology series, each chapter includes an introduction to the topic, a list of the necessary materials and reagents, reproducible step-by-step laboratory protocols, and tips on troubleshooting common problems and avoiding pitfalls. Authoritative and practical, Peptidomics:Methods and Strategies provides useful guidance for studies in the rapidly growing field of peptidomics. .
Biochemistry. --- Protein Science. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition
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This new edited volume in the Springer Subcellular Biochemistry Series presents a comprehensive, state-of-the-art overview of the proteomics of peroxisomes derived from mammalian, Drosophila, fungal, and plant origin, andcontains contributions from leading experts in the field. The development of sensitive proteomics and mass spectrometry technologies, combined with bioinformatics approaches now allow the identification of low-abundance and transient peroxisomal proteins and permits to identify the complete proteome of peroxisomes, with the consequent increase of our knowledge of the metabolic and regulatory networks of these important cellular organelles. The book lines-up with these developments and is organized in four sections including: (i) mass spectrometry-based organelle proteomics; (ii) prediction of peroxisomal proteomes; (iii) analysis of peroxisome proteome interaction networks; and (iv) peroxisomes in relation to other subcellular compartments. The editor Luis A. del Río is Professor ad honorem of the Spanish National Research Council (CSIC) in the Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry and Cell & Molecular Biology of Plants, at the Estación Experimental del Zaidín, Granada, Spain. Del Río’s research group focuses on the metabolism of reactive oxygen species (ROS), reactive nitrogen species (RNS) and antioxidants in plant peroxisomes, and the ROS- and RNS-dependent role of peroxisomes in plant cell signalling. The editor Michael Schrader is Professor of Cell Biology & Cytopathology in the Department of Biosciences at the University of Exeter, UK. Using mammalian peroxisomes as model organelles, Prof. Schrader and his team aim to unravel the molecular machinery and signalling pathways that mediate and regulate the formation, dynamics and abundance of these medically relevant cellular compartments.
Proteomics. --- Cytology. --- Biochemistry. --- Human genetics. --- Cell physiology. --- Cell Biology. --- Animal Biochemistry. --- Plant Biochemistry. --- Human Genetics. --- Cell Physiology. --- Cell function --- Cytology --- Physiology --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Cell biology --- Cellular biology --- Cells --- Cytologists --- Molecular biology --- Proteins --- Composition --- Cell biology. --- Plant biochemistry. --- Phytochemistry --- Plant biochemistry --- Plant chemistry --- Biochemistry --- Botany --- Phytochemicals --- Plant biochemical genetics
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This new edited volume in the Springer Subcellular Biochemistry Series presents a comprehensive, state-of-the-art overview of the proteomics of peroxisomes derived from mammalian, Drosophila, fungal, and plant origin, andcontains contributions from leading experts in the field. The development of sensitive proteomics and mass spectrometry technologies, combined with bioinformatics approaches now allow the identification of low-abundance and transient peroxisomal proteins and permits to identify the complete proteome of peroxisomes, with the consequent increase of our knowledge of the metabolic and regulatory networks of these important cellular organelles. The book lines-up with these developments and is organized in four sections including: (i) mass spectrometry-based organelle proteomics; (ii) prediction of peroxisomal proteomes; (iii) analysis of peroxisome proteome interaction networks; and (iv) peroxisomes in relation to other subcellular compartments. The editor Luis A. del Río is Professor ad honorem of the Spanish National Research Council (CSIC) in the Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry and Cell & Molecular Biology of Plants, at the Estación Experimental del Zaidín, Granada, Spain. Del Río’s research group focuses on the metabolism of reactive oxygen species (ROS), reactive nitrogen species (RNS) and antioxidants in plant peroxisomes, and the ROS- and RNS-dependent role of peroxisomes in plant cell signalling. The editor Michael Schrader is Professor of Cell Biology & Cytopathology in the Department of Biosciences at the University of Exeter, UK. Using mammalian peroxisomes as model organelles, Prof. Schrader and his team aim to unravel the molecular machinery and signalling pathways that mediate and regulate the formation, dynamics and abundance of these medically relevant cellular compartments.
Genetics --- Histology. Cytology --- General biochemistry --- Animal biochemistry --- Human genetics --- Biochemical engineering --- medische genetica --- proteomics --- bio-informatica --- biochemie --- genetica --- biotechnologie --- cytologie --- histologie --- planten --- moleculaire biologie --- proteïnen
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