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In Chaperonin Protocols, Christine Schneider has assembled a unique collection of readily reproducible protocols for the study of chaperonins, intracellular proteins critical to many biological processes. Written by experienced investigators who have successfully honed their methods to a fineness, the protocols focus on the purification of chaperonins from different species along with their corresponding cofactors, and on chaperonin activity assays for in vivo as well as in vitro work. Many activity assays are given for GroEL, which can also be applied to mitochrondrial Hsp60. There are also assays for the eukaryotic chaperonin TRiC and handy methods-for example, one for preparing labeled probes-that can be used for various purposes and prove helpful in numerous different procedures. Critically important to a greater understanding of such disorders as cystic fibrosis, Alzheimer's disease, and BSE, Chaperonin Protocols offers both novice and experienced investigators fast access to today's best and most productive chaperonin methods, all explained in step-by-step detail to ensure robust and reproducible results.

Molecular chaperones --- Purification --- Molecular biology. --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biophysics --- Biomolecules --- Systems biology --- Chaperone proteins --- Chaperones, Molecular --- Chaperonins --- Proteins --- Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition

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Durch die Fortentwicklung des Wirtschaftsumfeldes wird die permanenten Anpassungsfähigkeit von Unternehmen zu einer Voraussetzung für ihre langfristige Existenzsicherung. Besonders in Branchen mit weniger starkem Wachstumspotenzial müssen Betriebe Strategien entwickeln, um Wettbewerbsvorteile zu erzielen. Christine R. Schneider untersucht die Frage nach den Erfolgsfaktoren in der Gastronomie aus der Sicht der Industrieökonomietheorie, der ressourcenbasierten Theorie sowie der Entrepreneurship-Theorie mit besonderer Berücksichtigung des menschlichen und sozialen Kapitals des Unternehmers. Ausgehend von einer Perspektive des strategischen Managements und des Unternehmertums entwickelt und testet die Autorin ein theoretisches Modell im Rahmen einer quantitativen Untersuchung von St. Galler Gastronomiebetrieben. Die Autorin identifiziert den Unternehmer, eine Differenzierungsstrategie sowie die Erzielung virtueller Renten als kritische Erfolgsfaktoren in kleinen Dienstleistungsunternehmen.

Leadership. --- Management. --- Business Strategy/Leadership.

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Josephinism

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