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Capillary Electrophoresis: Methods and Protocols presents a selection of current capillary electrophoresis methods used to separate representative types of molecules and particles and in combination with different detection techniques. The volume is intended for beginners in the field and provides overviews of each technique and a starting point for the exploration of the defined literature on the different application topics. As a beginning text, each technique is explained in simple terms, assuming only minimal previous knowledge of the methods. A Notes section accompanies each chapter and addresses problems that may occur and how to overcome them. In Part I, Analyte-Oriented, contributors present methods and protocols based on sample type and size for inorganic and organic small ions, anionic, cationic, and neutral pollutants and pharmaceuticals, sugars, amino acids, nucleotides and DNA, and synthetic copolymers up to microorganisms. In Part II, Methods-Oriented, authors present the more methodological descriptions of particular and new CE techniques including CZE, MEKC/MECC, CEC, affinity CE, coating and online concentration techniques, single-cell analysis, and multidimensional techniques. Comprehensive and timely, Capillary Electrophoresis: Methods and Protocols is ideal for researchers looking for a beginning text on capillary electrophoresis methods.
SCIENCE --- Life Sciences / Biochemistry --- Capillary electrophoresis --- Electrophoresis, Capillary --- Methods --- Electrophoresis --- Investigative Techniques --- Electrochemical Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Chemistry Techniques, Analytical --- Animal Biochemistry --- Human Anatomy & Physiology --- Health & Biological Sciences --- Capillary electrophoresis. --- Capillary gel electrophoresis --- Capillary zone electrophoresis --- Life sciences. --- Human genetics. --- Cell biology. --- Life Sciences. --- Cell Biology. --- Human Genetics. --- Gel electrophoresis --- Zone electrophoresis --- Cytology. --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists
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This new edition presents principle methods in capillary electrophoresis (CE) separation involving CZE, MEKC, MECC, NACE, and corresponding hyphenated techniques to organic mass spectrometry and ICP-MS. Recent developments in the techniques of single cell analysis, as well as derivation, enantioseparation or the use of ionic liquids, and the use of CZE for the separation of living cells are also highlighted. This book discusses various application methods for the analysis of small ions, organic acids, amino acids, and (poly)saccharides to peptides that are shown with pollutants and biomarkers in food and health. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and thorough, Capillary Electrophoresis: Methods and Protocols, Second Edition covers a wide field of interests and will be especially great for beginners and students because of its combined focus on mini-reviews and application notes that will help them quickly get an overview of the field.
Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition
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Fourier transform spectroscopy. --- Mass spectrometry --- Methodology. --- Mass spectra --- Mass spectrograph --- Mass spectroscopy --- Mass spectrum analysis --- Mass (Physics) --- Nuclear spectroscopy --- Spectrum analysis --- Fourier transform interferometry --- Interference spectrometry --- Multiplex spectrometry --- Fourier transform optics
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Fourier transform spectroscopy. --- Mass spectrometry --- Methodology.
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